Chimeric pigs following blastocyst injection of transgenic porcine primordial germ cells

The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

“…SSEA-1 is an antigen found on mouse EC cells, ES cells and PGCs. The presence of SSEA-1 antigen in pig EG cells is similar to those findings of Takagi et al [18] and Mueller et al [13], that SSEA-1 is a good marker for pig PGCs and long term cultured PGCs. However, Takagi et al did not find the expression of SSEA-3 and SSEA-4 in pig PGCs and Mueller et al did not analyze both SSEA-3 and -4.…”

Effects of Protease Inhibitors and Antioxidants on In Vitro Survival of Porcine Primordial Germ Cells1

“…Additionally, SSEA-1 could identify PGCs cultured on feeder cells [17]. This and previous studies [21][22][23] found that isolated porcine PGCs, when cultured in vitro using a mixture of growth factors and feeder cells, grew in colonies with ES-like morphology and were AP and SSEA-1 positive (Fig. 2).…”

“…2). Previously, we and others [21][22][23] have demonstrated that PGC-derived cells can differentiate in vitro and contribute to the formation of various organs when introduced into a host blastocyst.…”

Culture of Porcine Embryonic Germ Cells in Serum-Supplemented and Serum-Free Conditions: The Effects of Serum and Growth Factors on Primary and Long-Term Culture

“…In this study PGC from embryos at days 24-26 of pregnancy were used because at this age the genital ridges are sufficiently developed for dissection and PGC numbers are adequate for culture. While it has been proven possible to establish EGC lines from porcine embryos at ages up to 28 days of pregnancy (Mueller et al, 1999), in our hands PGC have shown decreased proliferation potential in culture with increasing embryonic age past day 26 of pregnancy (unpublished observations).…”