Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology

Zhao, Keyan; Chen, Rulong; Duan, Wenhui; Meng, Lanhuan; Song, Hongmiao; Wang, Qing; Li, Jiangkuo; Xu, Xiangbin

doi:10.3389/fnut.2022.906227

Cited by 11 publications

(6 citation statements)

References 48 publications

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“…Totally, we identified 122 miRNA‐cleaved targets, including DCL1 , REV , ARF10, GRF4 , and GRAS24 , which were cleaved by miR162, miR165/miR166, miR160, miR396, and miR171, respectively (Figure 6b, Table S6). Among these targets, GRAS24 was previously shown to increase in expression in tomato when its endogenous Sly‐miR171d or Sly‐miR171e was silenced, resulting in improved chilling resistance (Xing et al., 2022; Zhao et al., 2022). Our GMUCT profiling validated the miR171 cleavage at GRAS24 in tomato.…”

Section: Resultsmentioning

confidence: 99%

Diurnal gene oscillations modulated by RNA metabolism in tomato

Zhang

Xue

et al. 2023

The Plant Journal

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SUMMARYDiurnal rhythms are known to regulate the expression of a large number of genes, coordinating plant growth and development with diel changes in light and temperature. However, the impact of RNA metabolism on rhythmic gene oscillations in plant is not yet fully understood. To address this question, we performed transcriptome and degradome profiling on tomato leaves at 6 time points during one 24 h cycle, using RNA‐seq and genome‐wide mapping of uncapped and cleavage transcripts (GMUCT). Time‐series profiling of RNA‐seq revealed 9342 diurnal‐oscillated genes, which were enriched in various metabolic processes. To quantify the general level of RNA degradation for each gene, we utilized the Proportion Uncapped (PU) metric, which represents the GMUCT/RNA‐seq ratio. Oscillated PU analysis revealed that 3885 genes were regulated by rhythmic RNA degradation. The RNA decay of these diurnal genes was highly coordinated with mRNA downregulation during oscillation, highlighting the critical role of internal transcription‐degradation balance in rhythmic gene oscillation. Furthermore, we identified 2190 genes undergoing co‐translational RNA decay (CTRD) with 5′ phosphate read ends enriched at the boundary of ribosomes stalling at translational termination sites. Interestingly, diurnal‐changed mRNAs with large amplitudes tended to be co‐translationally decay, suggesting that CTRD contributed to the rapid turnover of diurnal mRNAs. Finally, we also identified several genes, whose miRNA cleavage efficiency oscillated in a diurnal manner. Taken together, these findings uncovered the vital functions of RNA metabolism, including rhythmic RNA degradation, CTRD, and miRNA cleavage, in modulating the diurnal mRNA oscillations during diel change at post‐transcriptional level in tomato.

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Section: Resultsmentioning

confidence: 99%

Diurnal gene oscillations modulated by RNA metabolism in tomato

Zhang

Xue

et al. 2023

The Plant Journal

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“…In general, microRNAs (miRNAs) are a class of small, nonprotein coding RNA molecules that function as negative regulators of target gene messages (Hinske et al, 2010), and their negative modulations on target genes are essential for enhancing cold tolerance (Zhao et al, 2022). Xing et al investigated the mechanism of action of Sly-miR171d on chilling injury in tomato fruit.…”

Section: Genetic Improvement Of Cold Tolerance In Horticultural Cropsmentioning

confidence: 99%

Editorial: The physiology, molecular biology and biochemistry in ripening and stored fruit

Li,

Cao,

Yang

et al. 2023

Front. Plant Sci.

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Editorial on the Research TopicThe physiology, molecular biology and biochemistry in ripening and stored fruit Quality formation and ripening regulation of horticultural fruitsThe material basis and regulator underlying fruit development and ripening still remain challenges. Teixeira et al. showed that the exocarp of green and mature grape berries is rich in chloroplasts, and they applied proteomic analysis of chloroplasts from the Frontiers in Plant Science frontiersin.org 01

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“…Silencing of Sly-miR171d or Sly-miR171e with STTM was performed according to Yan et al [63]. Primary transgenic plant (T0) was generated by Agrobacterium tumefaciens-mediated transformation according to Zhao et al [22]. Three STTM-miR171d and STTM-miR171e lines were selected from the T1 and T2.…”

Section: Transformation Of Tomato Plantmentioning

confidence: 99%

“…It has been documented that SlGRAS24 is a key transcription factor coordinating the regulation of GA signaling pathways in tomato, and the HAM genes may be regulators of endogenous GA balance in SAM, RAM, and axillary meristem to control the maintenance of meristem and generation of organs [20]. In previous studies, it was found that silencing of miR171d and miR171e enhanced the cold tolerance of tomato fruit by targeting SlGRAS24 to regulate GA signaling [21,22].…”

Section: Introductionmentioning

confidence: 99%

Silencing of miR171d/e in tomato enhances salt tolerance of plant and improves fruit nutritional quality

Huang¹,

Xing²,

Shuhui³

et al. 2023

Preprint

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High salinity in soil affects the normal growth and development of crop. MicroRNA171 (miR171) plays a momentous role in plant resistance to adversity stress. The functions of Sly-miR171d and Sly-miR171e on growth of tomato and fruit nutritional quality under salt stress were studied. The results showed that the seed germination and seedling growth of tomato that silencing of Sly-miR171d/e by short tandem target mimic (STTM) were better than those of wild-type (WT). Silencing of Sly-miR171d/e seeds maintained a high germination rate, while WT tomato seeds barely germinated under 50 mM NaCl stress. Under 100 mM NaCl stress, silencing of Sly-miR171d/e increased the expression of their target gene SlGRAS24 with increasing duration of salt stress, while they enhanced the antioxidant activity, reduced reactive oxygen species (ROS) accumulation, and enhanced the salt tolerance of tomato plant by regulating gibberellin (GA) level. Moreover, the preharvest and postharvest fruit nutritional quality under salt stress was also studied, and the results showed that silencing of Sly-miR171d/e increased soluble solids, lycopene, total carotenoids, Vitamin C, organic acids and phenolic substances in tomato fruit. The present study demonstrated that silencing of Sly-miR171d/e enhanced plant salt tolerance through the GA pathway, and improved fruit nutritional quality under salt stress.

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Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology

Cited by 11 publications

References 48 publications

Diurnal gene oscillations modulated by RNA metabolism in tomato

Diurnal gene oscillations modulated by RNA metabolism in tomato

Editorial: The physiology, molecular biology and biochemistry in ripening and stored fruit

Silencing of miR171d/e in tomato enhances salt tolerance of plant and improves fruit nutritional quality

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