Chicken Cecum Immune Response to<i>Salmonella enterica</i>Serovars of Different Levels of Invasiveness

Berndt, Angela; Wilhelm, Anne; Jugert, Christiane; Pieper, Jana; Sachse, Konrad; Methner, Ulrich

doi:10.1128/iai.00695-07

Cited by 195 publications

(142 citation statements)

References 63 publications

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“…Outros estudos avaliaram a interação dessas células em agregados linfoides intestinais após infecção oral com Salmonella em aves jovens (Sasai et al 2000, Berndt et al 2007, Hemert et al 2007. As células T CD8+ desempenham um importante papel no combate das infecções primárias por salmonelas, assim como a resposta por IgA e leucócitos polimorfonucleados (Berndt & Methener 2001).…”

Section: Discussionunclassified

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

et al. 2015

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RESUMO.-Objetivou-se com o presente estudo comparar o efeito de diferentes sorovares de Salmonella na resposta imune local da mucosa do intestino de frangos de corte. Aos sete dias de idade, as aves foram desafiadas com os sorovares S. Enteritidis, S. Typhimurium, S. Senftenberg, S. Mbandaka e S. Minnesota. Foi observado que todos os sorovares testados foram capazes de colonizar o intestino das aves sendo possível o isolamento de Salmonella em suabes de cloaca, 48 h após inoculação. De maneira geral, as aves do grupo controle negativo, que não foram desafiados apresentaram quantidade significativamente menor de células imunológicas na mucosa intestinal do que as aves desafiadas. Porém, verificou--se que os sorovares de Salmonella, utilizados neste estudo, apresentaram diferentes efeitos sobre a dinâmica celular da mucosa do íleo e ceco e afetaram de modo diferente o ganho de peso e ganho médio diário das aves demonstrando distintos graus de patogenicidade. Os sorovares Enteritidis e Typhimurium apresentaram um efeito mais intenso tanto no desempenho quanto na mobilização de células imunoló-gicas na mucosa intestinal de frangos de corte. The study was designed to compare the effect of different Salmonella serovars in immune response across the count of CD8+ cells, CD4+ cells, goblet cells and macrophages in the gut mucosa of broilers. During the experimental inoculation at 7 day-old were used Salmonella enterica subspecies enterica sorovars Enteritidis, Typhimurium, Senftenberg, Mbandaka and Minnesota. It was observed that all serovars tested were capable of contaminating the poultry being possible counts of Salmonella in cloacal swabs, 48 h after inoculation and into the crop and cecum, at 14 and 20 day-old. Serovars tested had different effects on broiler performance assessed at 20 days. In the mucosa of the ileum and cecum of broilers, it was observed that some of the serotypes increased CD8 + cells, CD4 + cells, goblet cells and macrophages compared to the negative control group both at 14 and at 20 day-old. S. Enteritidis and S. Typhimurium are the serovars that showed the more intense effect in live performance and in the immune system of birds showing pathogenic characteristic; generally the broilers of the negative control showed significantly less immune cells on the intestinal mucosa than broilers inoculated experimentally. However, it was found that the Salmonella serovars used in this study had different effects on the cellular dynamics of the mucosa of the ileum and cecum and differently affect weight gain and average daily gain of poultry showing different levels of pathogenicity. Avaliação da resposta imunológica da mucosa intestinal

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Section: Discussionunclassified

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

et al. 2015

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“…Unlike noninvasive strains, invasive Salmonella strains penetrate through the epithelial lining to the lower parts of the lamina propria. Also, invasive strains are commonly isolated from parenhymatous organs (spleen, liver, ovaries) and a small number of bacteria become internalized by macrophages (Berndt et al, 2007). The survival in the acidic environment of the stomach is enabled by the activation of more than 50 acid tolerance response proteins (Bearson et al, 2006).…”

Section: The Pathogenesis Tissue Invasion and Immune Responsesmentioning

confidence: 99%

“…They trigger systemic and local immune response which is in good correlation with their virulence. Experimental work was conducted by Berndt et al, (2007) to measure the immune response in cecum after the infection of White Leghorn day old chickens with serovars Enteritidis, Typhimurium, Hadar and Infantis. At 2, 4 and 7 days post infection (pi) serovars Hadar and Infantis showed diminished invading capabilities for liver, compared to serovars Enteritidis and Typhimurium.…”

Section: The Pathogenesis Tissue Invasion and Immune Responsesmentioning

confidence: 99%

Salmonella spp. in poultry: a constant challenge and new insights

Velhner

Milanov

Kozoderović

2018

J Hellenic Vet Med Soc

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ABSTRACT. The knowledge about virulence mechanisms, resistance to antimicrobial agents and the biofilm formation ability of Salmonella spp. in poultry industry has been expanded over the years. However, in spite of the research efforts and significant investments to improve management systems in poultry industry, it has become evident that none of the methods applied in all stages of food production chain are 100% effective in eliminating Salmonella spp. Different serovars are manifesting different mechanisms of invasiveness which depend on their ability to invade lower zones of the lamina propria, their ability to gain accesses to parenchymatous organs and survive in macrophages. The ubiquitous nature of Salmonella spp. due to their adaptation to animal and plant hosts, as well as their survival in hostile environments and their enhanced capacity to produce biofilms, contribute to a long lasting contamination of the environment, feed and animals. The emergency and spread of antimicrobial resistances in Salmonella spp. raise additional concerns.

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“…The Th2 cytokine IL-4 was reported to enhance the host defence against Salmonella (Stoycheva & Murdjeva, 2005;Berndt et al, 2007). Isolate LB1 or combined Lactobacillus isolates tested in the current study could both attenuate the Salmonella-induced reduction of IL-4 in the ileum and spleen 3 days after infection.…”

Section: Discussionmentioning

confidence: 77%

Modulation of cytokine gene expression by selectedLactobacillusisolates in the ileum, caecal tonsils and spleen ofSalmonella-challenged broilers

Kulkarni³

et al. 2015

Avian Pathology

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Chicken Cecum Immune Response toSalmonella entericaSerovars of Different Levels of Invasiveness

Cited by 195 publications

References 63 publications

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

Salmonella spp. in poultry: a constant challenge and new insights

Modulation of cytokine gene expression by selectedLactobacillusisolates in the ileum, caecal tonsils and spleen ofSalmonella-challenged broilers

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