Chick embryonic Schwann cells migrate anodally in small electrical fields

McKasson, Marilyn J.; Huang, Ling; Robinson, Kenneth R.

doi:10.1016/j.expneurol.2008.02.015

Cited by 51 publications

(44 citation statements)

References 17 publications

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“…A wide variety of cell types such as karatocytes, epithelial cells, avian glia, and hippocampal neurons are sensitive to exogenous electrical stimulation and exhibit varied responses such as galvanotaxis toward the cathode or neuronal turning toward the anode or cathode. 52,[78][79][80][81][82][83][84][85][86] In this work, primary fibroblasts and an endothelial cell line exhibited no visible changes in morphology during, immediately after, or poststimulation. Unlike electrically stimulated Schwann cells, these prestimulated nonneural support cells did not enhance or inhibit neurite extension (Fig.…”

Section: Koppes Et Almentioning

confidence: 99%

Electrical Stimulation of Schwann Cells Promotes Sustained Increases in Neurite Outgrowth

Koppes

Nordberg²,

Paolillo³

et al. 2013

Tissue Engineering Part A

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Endogenous electric fields are instructive during embryogenesis by acting to direct cell migration, and postnatally, they can promote axonal growth after injury (McCaig 1991, Al-Majed 2000. However, the mechanisms for these changes are not well understood. Application of an appropriate electrical stimulus may increase the rate and success of nerve repair by directly promoting axonal growth. Previously, DC electrical stimulation at 50 mV/mm (1 mA, 8 h duration) was shown to promote neurite outgrowth and a more pronounced effect was observed if both peripheral glia (Schwann cells) and neurons were co-stimulated. If electrical stimulation is delivered to an injury site, both the neurons and all resident non-neuronal cells [e.g., Schwann cells, endothelial cells, fibroblasts] will be treated and this biophysical stimuli can influence axonal growth directly or indirectly via changes to the resident, non-neuronal cells. In this work, non-neuronal cells were electrically stimulated, and changes in morphology and neuro-supportive cells were evaluated. Schwann cell response (morphology and orientation) was examined after an 8 h stimulation over a range of DC fields (0-200 mV/mm, DC 1 mA), and changes in orientation were observed. Electrically prestimulating Schwann cells (50 mV/mm) promoted 30% more neurite outgrowth relative to co-stimulating both Schwann cells with neurons, suggesting that electrical stimulation modifies Schwann cell phenotype. Conditioned medium from the electrically prestimulated Schwann cells promoted a 20% increase in total neurite outgrowth and was sustained for 72 h poststimulation. An 11-fold increase in nerve growth factor but not brain-derived neurotrophic factor or glial-derived growth factor was found in the electrically prestimulated Schwann cell-conditioned medium. No significant changes in fibroblast or endothelial morphology and neuro-supportive behavior were observed poststimulation. Electrical stimulation is widely used in clinical settings; however, the rational application of this cue may directly impact and enhance neuro-supportive behavior, improving nerve repair.

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Section: Koppes Et Almentioning

confidence: 99%

Electrical Stimulation of Schwann Cells Promotes Sustained Increases in Neurite Outgrowth

Koppes

Nordberg²,

Paolillo³

et al. 2013

Tissue Engineering Part A

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show abstract

“…Similar to neurons, astrocytes and Schwann cells undergo oriented growth in response to an EF, and Schwann cells migrate rapidly anodally in EFs as low as 3 mV/mm McKasson et al, 2008). Glial and neuronal cells are tightly related functionally and are often interconnected by communicating gap junctions.…”

Section: The Future For Electrically Driven Neuronal Migrationmentioning

confidence: 99%

Electrical dimensions in cell science

McCaig

Song

Rajnicek

2009

Journal of Cell Science

283

277

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“…18 In contrast, breast cancer cells and some endothelial cells migrate to the opposite direction, which is toward the anode of the field. [19][20][21][22] Some cells also display additional atypical shape changes that accompany the directional migration phenotype. Mouse fibroblasts depict, for instance, a striking shape elongation perpendicular to the EF and start migrating to the cathode of the EF.…”

Section: Discussion Of Findings and Relevant Literaturementioning

confidence: 99%