Chemotyping of new hop ( Humulus lupulus  L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry

Yan, Dan Dan; Wong, Yong Foo; Tedone, Laura; Shellie, Robert A.; Marriott, Philip J.; Whittock, Simon P.; Koutoulis, Anthony

doi:10.1016/j.chroma.2017.08.020

Cited by 30 publications

(28 citation statements)

References 34 publications

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“…Several ferulic acid derivatives were found, and their concentration was higher in bioprocessed BSG, in which the extensive degradation of the cell walls caused the increase of the total phenols extractability. Catechin, epicatechin, quercetin, and safrole, this latter previously identified in hop (Yan et al, 2018), were found in both free and bound phenols fraction. During the industrial brewing process, the trub separated after boiling the wort is also added to BSG, therefore, it is possible to find hop components.…”

Section: Discussionmentioning

confidence: 60%

Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides

Verni

Pontonio

Krona³

et al. 2020

Front. Microbiol.

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Brewers' spent grain (BSG) is the major by-product of the brewing industry which remain largely unutilized despite its nutritional quality. In this study, the effects of fermentation on BSG antioxidant potential were analyzed. A biotechnological protocol including the use of xylanase followed by fermentation with Lactiplantibacillus plantarum (Lactobacillus plantarum) PU1, PRO17, and H46 was used. Bioprocessed BSG exhibited enhanced antioxidant potential, characterized by high radical scavenging activity, long-term inhibition of linoleic acid oxidation and protective effect toward oxidative stress on human keratinocytes NCTC 2544. Immunolabelling and confocal laser microscopy showed that xylanase caused an extensive cell wall arabinoxylan disruption, contributing to the release of bound phenols molecules, thus available to further conversion through lactic acid bacteria metabolism. To clarify the role of fermentation on the antioxidant BSG potential, phenols were selectively extracted and characterized through HPLC-MS techniques. Novel antioxidant peptides were purified and identified in the most active bioprocessed BSG.

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Section: Discussionmentioning

confidence: 60%

Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides

Verni

Pontonio

Krona³

et al. 2020

Front. Microbiol.

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“…Essential oils, typically obtained by hydrodistillation, are mainly injected directly, although a sample preparation step beforehand can be beneficial to increase selectivity. For example, an additional liquid‐liquid extraction combined with high‐resolution GC×GC‐MS (QTOF) was used for the detailed chemotypic profiling of essential oils of different hop genotypes [109]. Bendif et al .…”

Section: Non‐targeted Analysis Applicationsmentioning

confidence: 99%

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Franchina

Zanella

Dubois

et al. 2020

J of Separation Science

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In this review, we consider and discuss the affinity and complementarity between a generic sample preparation technique and the comprehensive two‐dimensional gas chromatography process. From the initial technical development focus (e.g., on the GC×GC and solid‐phase microextraction techniques), the trend is inevitably shifting toward more applied challenges, and therefore, the preparation of the sample should be carefully considered in any GC×GC separation for an overreaching research. We highlight recent biomedical, food, and plant applications (2016–July 2020), and specifically those in which the combination of tailored sample preparation methods and GC×GC–MS has proven to be beneficial in the challenging aspects of non‐targeted analysis. Specifically on the sample preparation, we report on gas‐phase, solid‐phase, and liquid‐phase extractions, and derivatization procedures that have been used to extract and prepare volatile and semi‐volatile metabolites for the successive GC×GC analysis. Moreover, we also present a milestone section reporting the early works that pioneered the combination of sample preparation techniques with GC×GC for non‐targeted analysis.

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“…The application of high resolution (HR) mass spectrometry to GC × GC metabolomics is in its infancy, representing only three percent of the metabolomics studies published since 2012 (Appendix Table) [20-22]. The impact of using HRMS is greatest for untargeted metabolomics, where the accurate mass data provides molecular formulae for unknown compounds.…”

Section: Methods Development For Gc × Gc Metabolomicsmentioning

confidence: 99%

Advances in the application of comprehensive two-dimensional gas chromatography in metabolomics

Keppler

Jenkins

Davis

et al. 2018

TrAC Trends in Analytical Chemistry

110

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Due to excellent separation capacity for complex mixtures of chemicals, comprehensive two-dimensional gas chromatography (GC × GC) is being utilized with increasing frequency for metabolomics analyses. This review describes recent advances in GC × GC method development for metabolomics, organismal sampling techniques compatible with GC × GC, metabolomic discoveries made using GC × GC, and recommendations and best practices for collecting and reporting GC × GC metabolomics data.

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Chemotyping of new hop ( Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry

Cited by 30 publications

References 34 publications

Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides

Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides

The role of sample preparation in multidimensional gas chromatographic separations for non‐targeted analysis with the focus on recent biomedical, food, and plant applications

Advances in the application of comprehensive two-dimensional gas chromatography in metabolomics

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