Chemotaxis of T-cells after infection of human choroid plexus papilloma cells with Echovirus 30 in an in vitro model of the blood–cerebrospinal fluid barrier

Clinical implications of leukocyte infiltration at the choroid plexus in (neuro)inflammatory disorders

“…For example, the chemokine CCL20, a ligand for CCR6, is expressed by the CP and has been suggested to mediate the initial step of Th17 cell invasion across the BCSFB into the CNS during EAE [42]. Chemokines are crucial for integrindependent adhesion and transmigration of naive and memory lymphocytes, but the role of extracellular chemokines in the transmigration of lymphocytes is under discussion [43,44]. Research indicates that Th1 and type 1 cytotoxic T cells express integrins that bypass chemokine signals [43].…”

“…PMNs (e.g., neutrophils) and monocytes dominate bacterial meningitis, whereas lymphocytes (especially T lymphocytes) are increased in viral CNS infections [18,[60][61][62][63][64]. Enterovirus (EV) is the most common pathogen causing viral meningitis, which is less fatal than septic meningitis and is associated with T cell migration at the BBB and BCSFB [44,65]. In the CSF of children with aseptic EV meningitis, concentrations of the chemokines CXCL10 and CXCL11 are increased, implying leukocyte infiltration [44].…”

“…Enterovirus (EV) is the most common pathogen causing viral meningitis, which is less fatal than septic meningitis and is associated with T cell migration at the BBB and BCSFB [44,65]. In the CSF of children with aseptic EV meningitis, concentrations of the chemokines CXCL10 and CXCL11 are increased, implying leukocyte infiltration [44]. However, EV30 infection of an in vitro CPE culture led only to a minor enhancement of T lymphocyte transmigration [44].…”

“…In the CSF of children with aseptic EV meningitis, concentrations of the chemokines CXCL10 and CXCL11 are increased, implying leukocyte infiltration [44]. However, EV30 infection of an in vitro CPE culture led only to a minor enhancement of T lymphocyte transmigration [44]. From a therapeutic point of view, an interesting study was done in a cytokine-induced meningitis model, in which mice were systemically injected with monoclonal antibody BV11 to block the junctional adhesion molecule (JAM) [66].…”

Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier

“…HIBCPP cells were obtained from a CP papilloma of the lateral ventricle from a 29-year-old woman (8) and have been established as a functional model of the BCSFB presenting a high TEER and low permeability for macromolecules (9). Subsequently, HIBCPP cells have been used as an in vitro model of the BCSFB to analyse origin and development of bacterial and viral meningitis (9)(10)(11)(12)(13). Still, the HIBCPP cell line has not yet been characterized with respect to transport protein expression and function, preventing an assessment concerning their suitability for pharmacological research.…”