Chemopreventive potential of chrysin in 7,12-dimethylbenz(a)anthracene-induced hamster buccal pouch carcinogenesis

Abstract

Introduction:Chemoprevention, an emerging, appealing, and innovative approach in experimental oncology, deals with the inhibition, prevention or suppression of carcinogenesis, using natural products or synthetic derivatives. The aim of the present study is to investigate the chemopreventive potential of chrysin during 7,12-dimethylbenza[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Materials and Methods: Oral squamous cell carcinoma was developed in the buccal pouch of Syrian golden hamsters by painting them with 0.5 percent DMBA in liquid paraffin thrice a week, for 14 weeks. The status of lipid peroxidation, antioxidants, and phase I and II detoxification agents were utilized as biochemical end points, to assess the chemopreventive efficacy of chrysin in DMBA-induced hamster buccal pouch carcinogenesis. Results: In the present study, 100% tumor formation with marked abnormalities in the status of lipid peroxidation, antioxidants, and detoxification agents was noticed in hamsters treated with DMBA alone. Oral administration of chrysin at a dose of 250 mg/kg bw to DMBA-treated hamsters significantly reduced the tumor incidence and tumor size as well as reverted the status of the above-mentioned biochemical markers during DMBA-induced hamster buccal pouch carcinogenesis. Conclusion: Chrysin has the potential to delay rather than inhibit tumor formation as evidenced by the tumor formation in two of the DMBA + chrysin-treated hamsters, during DMBA-induced hamster buccal pouch carcinogenesis.

Avocado Extract Inhibits 7, 12-Dimethylbenz[a]anthracene (DMBA)- Induced Carcinogenesis in Hamster Cheek Pouches

“…Similar effects have been demonstrated in DMBA/HCP models [20,22,[38][39][40][41][42][43][44]. This effect may be attributed to their ability to regulate multiple key cell cycle genes leading to inhibition of cell proliferation [45].…”

A Review on Therapeutic Potential of Piper nigrum L. (Black Pepper): The King of Spices

“…Piper nigrum (Black Pepper) or pure compound ''Piperine'' exhibits many more Pharmacological activities like antihypertensive, antiplatelets, antipyretic, antispasmodic, antifungal, anti-apoptotic, anti-metastatic, antimutagenic, anti-spermatogenic, anti-Colon toxin, anti-asthmatics, anti-anxiety, antithyroids, antifungal, insecticidal and larvicidal activities etc [1][2][3][4][5].…”

“…It is widely used in different traditional systems of medicine like Ayurvedic and Unani System of medicines [1,2]. Piperine exhibits diverse pharmacological activities like antihypertensive and antiplatelets [3], antioxidant, antitumor [4], antiasthmatics [5], antipyretic, analgesic, anti-inflammatory, anti-diarrheal, antispasmodic, anxiolytic, antidepressants [6], hepato-protective [7], immuno-modulatory, antibacterial, antifungal, anti-thyroids, antiapoptotic, anti-metastatic, antimutagenic, anti-spermatogenic, antiColon toxin, insecticidal and larvicidal activities etc. Piperine has been found to enhance the therapeutic efficacy of many drugs, vaccines and nutrients by increasing oral bioavailability by inhibiting various metabolising enzymes [8].…”

Carcinogenesis in mammalian oral mucosa from the perspective of biomedical research

“…Chomik jako model OSCC wykorzystywany jest do badań nad wpływem czynników onkogennych, hormonów i sposobu odży-wiania na kancerogenezę, a także jako źródło informacji na temat możliwości zastosowania określonych markerów molekularnych i biologicznych. Pozwala to na śledzenie zachodzących zmian genetycznych, molekularnych i immunologicznych w czasie kancerogenezy (41, 42), a także na testowanie potencjalnych związków przeciwnowotworowych (24,39,42).…”