Chemopreventive effect of flavonoids from Ougan (Citrus reticulata cv. Suavissima) fruit against cancer cell proliferation and migration

Zhang, Jiukai; Wu, Yeping; Zhao, Xiaoyong; Luo, Fenglei; Li, Xian; Zhu, Hong; Sun, Chongde; Chen, Kunsong

doi:10.1016/j.jff.2014.08.006

Cited by 57 publications

(35 citation statements)

References 32 publications

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“…Each part of the fruit tissue was lyophilized and ground in a laboratory mill. The methods of preparing extracts were based on Zhang et al with some modifications [31]. The ground powder of the two fruit parts was extracted with 20 mL of 80% methanol for 30 min with ultrasound, frequency 60 kHz and power 30 W, respectively.…”

Section: Methodsmentioning

confidence: 99%

Anti-Obesity and Hypoglycemic Effects of Poncirus trifoliata L. Extracts in High-Fat Diet C57BL/6 Mice

et al. 2016

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The present study investigated the possible anti-obesity and hypoglycemic effects of Poncirus trifoliata L. extracts. Mature fruit were divided into flavedo (PF) and juice sacs (PJ), and extracts from them were tested on C57BL/6 mice fed a high-fat diet (HFD) for thirteen weeks. Both fruit extracts (40 mg/kg body weight, respectively) showed anti-obesity and hypoglycemic effects. Consumption of PF and PJ extracts reduced body weight by 9.21% and 20.27%, respectively. Liver and adipose weights, fasting glucose, serum triglyceride (TG), and low density lipoprotein cholesterol (LDL-c) levels decreased significantly, while serum high density lipoprotein cholesterol (HDL-c) and oral glucose tolerance levels increased significantly in response to two fruit extracts. These effects were due in part to the modulation of serum insulin, leptin, and adiponectin. Furthermore, transcript levels of fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1) were reduced while those of carnitine palmitoyltransferase 1α (CPT1α) and insulin receptor substrate 2 (IRS2) were increased in the liver of C57BL/6 mice, which might be an important mechanism affecting lipid and glucose metabolism. Taken together, P. trifoliata fruit can be potentially used to prevent or treat obesity and associated metabolic disorders.

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Section: Methodsmentioning

confidence: 99%

Anti-Obesity and Hypoglycemic Effects of Poncirus trifoliata L. Extracts in High-Fat Diet C57BL/6 Mice

et al. 2016

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“…The main by‐product of citrus fruit processing plants is citrus peel, which constitutes a potential environmental pollutant. Citrus fruit peel is a major source of flavanones (Zhang et al, ), which have numerous health benefits including hypoglycemic (De Moraes Barros, De Castro Ferreira, & Genovese, ), antioxidant (Barreca, Bellocco, Caristi, Leuzzi, & Gattuso, ), cardio‐protective (Yamada et al, ), anti‐diabetic (Oboh & Ademosun, ), anti‐cancer (Zhang et al, ), anti‐inflammatory, and anti‐lipidemic effects (Park, Choi, Eom, & Choi, ) both in vitro and in vivo (Chinapongtitiwat, Jongaroontaprangsee, Chiewchan, & Devahastin, ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and purification of four flavanones from peel of Citrus changshanensis

Liu

Han

2017

J Food Process Preserv

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Four flavanone glycosides, i.e., neoeriocitrin, naringin, hesperidin, and neohesperidin, were extracted, separated, and purified from the peel of Citrus changshanensis using homogenate extraction; AB‐8 macroporous resin column chromatography, and high‐speed counter‐current chromatography (HSCCC). Homogenate extraction was effective in the extraction of flavanones (yield: 437 mg g−1), and HSCCC was successful in the separation of flavanones. Ethyl acetate‐butanol‐water (3:2:5, v/v/v) was selected to separate naringin from neohesperidin. Neoeriocitrin and hesperidin were purified with chloroform‐methanol‐n‐butanol‐water (4:3:1:3, v/v/v/v). The compounds purified by HSCCC were analyzed by high‐performance liquid chromatography and liquid chromatography‐mass spectrometry. The purity of neoeriocitrin, hesperidin, naringin, and neohesperidin extracted from C. changshanensis peel was 95.47, 99.62, 99.21, and 98.45%, respectively. Practical applications Homogenate extraction represents an adequate flavanone extraction method. The combination of homogenate extraction and HSCCC is effective for the isolation and purification of flavanone compounds from citrus peel. Our developed method will allow an in‐depth characterization of flavanones from C. changshanensis and the comprehensive utilization of citrus by‐products to reduce environmental pollution and maximize the economic value added.

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“…Recent studies have focused on the anti-cancer activity of flavonoids isolated from plants (Park et al, 2014; Zhang et al, 2014; Zivkovic et al, 2014). Flavonoids are natural polyphenols present in many different foods, especially fruits and vegetables.…”

Section: Introductionmentioning

confidence: 99%

Dietary compounds galangin and myricetin suppress ovarian cancer cell angiogenesis

Huang

Chen

Rojanasakul

et al. 2015

Journal of Functional Foods

111

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Galangin and myricetin are flavonoids isolated from vegetables and fruits which exhibit antiproliferative activity in human cancer cells. In this study, their anti-angiogenic effects were investigated with in vitro (HUVEC) and in vivo (CAM) models, which showed that galangin and myricetin inhibited angiogenesis induced by OVCAR-3 cells. The molecular mechanisms through which galangin and myricetin suppress angiogenesis were also studied. It was observed that galangin and myricetin inhibited secretion of the key angiogenesis mediator vascular endothelial growth factor (VEGF) and decreased levels of p-Akt, p-70S6K and hypoxia-inducible factor-1α (HIF-1α) proteins in A2780/CP70 and OVCAR-3 cells. Transient transfection experiments showed that galangin and myricetin inhibited secretion of VEGF by the Akt/p70S6K/ HIF-1α pathway. Moreover, a novel pathway, p21/HIF-1α/VEGF, was found to be involved in the inhibitory effect of myricetin on angiogenesis in OVCAR-3 cells. These data suggest that galangin and myricetin might serve as potential anti-angiogenic agents in the prevention of ovarian cancers dependent on new blood vessel networks.

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Chemopreventive effect of flavonoids from Ougan (Citrus reticulata cv. Suavissima) fruit against cancer cell proliferation and migration

Cited by 57 publications

References 32 publications

Anti-Obesity and Hypoglycemic Effects of Poncirus trifoliata L. Extracts in High-Fat Diet C57BL/6 Mice

Anti-Obesity and Hypoglycemic Effects of Poncirus trifoliata L. Extracts in High-Fat Diet C57BL/6 Mice

Isolation and purification of four flavanones from peel of Citrus changshanensis

Dietary compounds galangin and myricetin suppress ovarian cancer cell angiogenesis

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