Chemoenzymatic approaches for exploring structure–activity relationship studies of bioactive natural products

Li, Fuzhuo; Deng, Heping; Renata, Hans

doi:10.1038/s44160-023-00358-8

Cited by 10 publications

(5 citation statements)

References 63 publications

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“…This evolution has also been accompanied by a notable rise in the applications of more specialized biosynthetic enzymes in synthetic work. Furthermore, there has also been an increased interest in employing this strategy in the preparation of natural product analogs for useful structure–activity relationship profiling, which is reviewed in depth elsewhere …”

Section: Discussionmentioning

confidence: 99%

Charting the Evolution of Chemoenzymatic Strategies in the Syntheses of Complex Natural Products

et al. 2023

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Bolstered by recent advances in bioinformatics, genetics, and enzyme engineering, the field of chemoenzymatic synthesis has enjoyed a rapid increase in popularity and utility. This Perspective explores the integration of enzymes into multistep chemical syntheses, highlighting the unique potential of biocatalytic transformations to streamline the synthesis of complex natural products. In particular, we identify four primary conceptual approaches to chemoenzymatic synthesis and illustrate each with a number of landmark case studies. Future opportunities and challenges are also discussed.

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Section: Discussionmentioning

confidence: 99%

Charting the Evolution of Chemoenzymatic Strategies in the Syntheses of Complex Natural Products

et al. 2023

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“…Discovering and characterizing novel enzyme functions and pathways from nature, as well as engineering and evolving enzymes which catalyze reactions that are new to nature [228,229], are important for extending the frontiers of biocatalytic reactions in syntheses. Unlocking the power of enzymes can transform the synthesis of metabolites, natural products, and non-natural small molecules derived thereof in various ways, from individual enzymatic reactions in chemoenzymatic synthesis to their full utilization in enzymatic total synthesis [230][231][232].…”

Section: Discussionmentioning

confidence: 99%

Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Wohlgemuth

2023

Metabolites

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Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis, and structural identification, have been involved in numerous life science milestones. The renewed focus on the small molecule domain of biological cells has also created an increasing awareness of the rising gap between the metabolites identified and the metabolites which have been prepared as pure compounds. The design and engineering of resource-efficient and straightforward synthetic methodologies for the production of the diverse and numerous metabolites and metabolite-like compounds have attracted much interest. The variety of metabolic pathways in biological cells provides a wonderful blueprint for designing simplified and resource-efficient synthetic routes to desired metabolites. Therefore, biocatalytic systems have become key enabling tools for the synthesis of an increasing number of metabolites, which can then be utilized as standards, enzyme substrates, inhibitors, or other products, or for the discovery of novel biological functions.

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“…Discovering and characterizing novel enzyme functions and pathways from nature as well as engineering and evolving enzyme which catalyze reactions new to nature [187,188] are important for extending the frontiers of biocatalytic reactions in synthesis. Unlocking the power of enzymes can transform the synthesis of metabolites, natural products and non-natural small molecules derived thereof in various ways, from individual enzymatic reactions in chemoenzymatic synthesis to their full utilization in enzymatic total synthesis [189][190][191]. Many roads can lead to discovering novel e n z y m e s f r o m n a t u r e , s u c h a s g u i d i n g t h e c o r r e c t a s s i g n m e n t o f e n z y m e f u n c t i o n s t o g e n e annotations and domains of unknown functions on genomes [192][193][194], unlocking natural product biosynthetic enzymes from metagenomes [195], or identifying missing enzymes in biosynthetic pathways to metabolites [196].…”

Section: Future Directionsmentioning

confidence: 99%

Synthesis of Metabolites and Metabolite-Like Compounds Using Biocatalytic Systems

Wohlgemuth

2023

Preprint

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Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis and structural identification, have been involved in numerous life science milestones. The renewed focus on the small molecule domain of biological cells has also created an increasing awareness of the rising gap between the metabolites identified and the metabolites which have been prepared as pure compounds. Due to the large number and molecular diversity of metabolites the design and engineering of resource-efficient and straightforward synthetic methodologies for their production have attracted much interest. The variety of metabolic pathways in biological cells provide a wonderful blueprint for designing simplified and resource-efficient synthetic routes to desired metabolites. Therefore, biocatalytic systems have become key enabling tools for the synthesis of an increasing number of metabolites, which can then be utilized as standards, enzyme substrates, inhibitors or products, or for the discovery of novel biological functions.

show abstract

Chemoenzymatic approaches for exploring structure–activity relationship studies of bioactive natural products

Cited by 10 publications

References 63 publications

Charting the Evolution of Chemoenzymatic Strategies in the Syntheses of Complex Natural Products

Charting the Evolution of Chemoenzymatic Strategies in the Syntheses of Complex Natural Products

Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Synthesis of Metabolites and Metabolite-Like Compounds Using Biocatalytic Systems

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