ChemInform Abstract: Recent Organometallic Nitrosyl Chemistry

Richter‐Addo, George B.; Legzdins, Peter

doi:10.1002/chin.198913364

Cited by 113 publications

(203 citation statements)

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“…As a small ligand, nitric oxide has long been a useful probe of iron and heme complexes (27), and relevant analytical methods are IR (28,28), EPR (30,31), and Mössbauer (32) spectroscopies. NO is a radical which upon coordination converts an overall even-electron system to an odd-electron system.…”

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confidence: 99%

Spectroscopic Characterization of the NO Adduct of Hydroxylamine Oxidoreductase

et al. 2002

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Hydroxylamine oxidoreductase (HAO) from the autotrophic nitrifying bacterium Nitrosomonas europaea catalyzes the oxidation of NH 2 OH to NO 2 -. The enzyme contains eight hemes per subunit which participate in catalysis and electron transport. NO is found to bind to the enzyme and inhibit electron flow to the acceptor protein, cytochrome c 554 . NO is found to oxidize either partially or fully reduced HAO, but NO will not reduce ferric HAO. Since NO can be reduced but not oxidized to product by HAO, NO is not considered to be a long-lived intermediate in the catalytic mechanism. Substrate oxidation occurs in the presence of bound NO or cyanide, suggesting a second interaction site for substrate with HAO and providing a means for recovery of the NO-inhibited form of the enzyme. Upon addition of NO to oxidized HAO, the integer-spin EPR signal from the active site vanishes, an IR band from NO appears at 1920 cm -1 , and a diamagnetic quadrupole iron doublet appears in Mössbauer spectroscopy with δ ) 0.06 mm/s and ∆Eq ) 2.1 mm/s. The NO stretching frequency and Mössbauer parameters are characteristic of an {FeNO} 6 heme complex. New Mössbauer data on ferric myoglobin-NO are also presented for comparison. The results indicate that NO binds to heme P460 and that the loss of the integer-spin EPR signal is due to the conversion of heme P460 to a diamagnetic S ) 0 state and concomitant loss of magnetic interaction with neighboring heme 6. In previous studies where the heme P460-heme 6 interaction was affected by substrate or cyanide binding, a signal attributable to heme 6 was not observable. In contrast, in this work, the NO-induced loss of the signal is accompanied by the appearance of a previously unobserved large g max (or HALS) low-spin EPR signal from heme 6.

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confidence: 99%

Spectroscopic Characterization of the NO Adduct of Hydroxylamine Oxidoreductase

et al. 2002

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“…29,34 It has been reported that when solutions of {MNO} 6 complexes are made alkaline, 36,40 a product with absorption band from 300 -400 nm is observed. 31,52,53 Additionally, this transformation is fully reversible upon addition of acid.…”

Section: (Imn)] + With Concomitant Production Of Cis-[ru(no)(bpy) 2 (mentioning

confidence: 99%

“…[27][28][29][30][31][32][33][34] These species exhibit quite distinct overall charge, which can strongly alter interaction with reverse phase column.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Investigation of Ruthenium Nitrosyl Complex: No Interconversion and Reactions With Biological Reductants

Silva¹,

Penha²,

Alencar³

et al. 2018

Quim. Nova

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3 complex. Chromatographic studies were carried out and showed that immediately after nitrite complex was dissolved only one species was present with retention time(t R ) of 6.81 minutes. Addition of H 3 O + to nitrite complex led to the formation of one major peak with t R of 3.92 min supporting nitrosyl complex formation. The reaction of nitrosyl complex with cysteine was also monitored by HPLC and it showed clearly the formation and followed decrease of a peak at 3.38 minute with maximum absorption at 380 nm, consistent with an intermediate complex. Later, it was observed the appearance of a peak at 4.15 minute with absorption band at 470 nm. In contrast to the reaction with cysteine, methionine did not show the formation of any intermediate. The use of HPLC was an important tool to support mechanistic assumptions for nitrosyl reactions.

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“…O óxido nítrico é uma molécula paramagnética sendo, nas condições normais de temperatura e pressão (CNTP), um gás incolor e termodinamicamente instável quando comparado aos gases N2 e O2 (RICHTER-ADDO & LEGZDINS, 1992). Sua solubilidade em meio aquoso é 2,1 × 10 -3 mol L -1 (MORI & BERTOTTI, 2000).…”

Section: Propriedades Físico-químicas Do óXido Nítricounclassified

Reatividade química e fotoquímica de complexos nitrosilos de rutênio do tipo [Ru(terpy)(L)NO]n+

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ChemInform Abstract: Recent Organometallic Nitrosyl Chemistry

Cited by 113 publications

References 1 publication

Spectroscopic Characterization of the NO Adduct of Hydroxylamine Oxidoreductase

Spectroscopic Characterization of the NO Adduct of Hydroxylamine Oxidoreductase

Chromatographic Investigation of Ruthenium Nitrosyl Complex: No Interconversion and Reactions With Biological Reductants

Reatividade química e fotoquímica de complexos nitrosilos de rutênio do tipo [Ru(terpy)(L)NO]n+

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