Chemiluminescent sensor for hydrogen sulfide in rat brain microdialysis based on target-induced horseradish peroxidase deactivation

Abstract

The detection of hydrogen sulfide (H2S) is important due to its role in the diagnosis of many diseases since H2S is involved in the protection of neurons from oxidative stress and neuronal transmission modulation in the brain.

A Reactive Oxygen Species (ROS) Activated Hydrogen Sulfide (H2S) Donor with Self-Reporting Fluorescence

“…Precise detection of the released H 2 S and delivery of H 2 S to the desired sites is critical in this field. However, the precise detection of the released H 2 S still remains a challenge. ,,− Although a few chemiluminescent probes have been developed to detect and visualize the H 2 S in vitro or in vivo, ,− most of the colorimetry, electrochemical analysis, and gas chromatography approaches to detect the H 2 S molecules were limited due to their insufficient sensitivities . The key advantages of the fluorescence method included better sensitivity, less invasiveness, more convenience, and offers high sensitivity as well as real-time imaging. − On the other hand, almost all fluorescent probes are required to react with hydrogen sulfide first to generate fluorescence imaging. ,, Engineering fluorescent donors with precise triggers that do not irreversibly consume the analyte would be highly favorable.…”

In Vivo Detection of Hydrogen Sulfide in Brain and Cell

“…In different CL systems, the luminol and hydrogen peroxide reactions with horseradish peroxidase (HRP) as catalyst have been used because of their simplicity and enhanced CL signal intensity. Recently, this triple system (luminol/H 2 O 2 /HRP) has been applied by Fang et al [70] to the determination of H 2 S, in which H 2 S inactivates HRP and thus quenches the system in a quantitative manner with CL (Figure 7A). The CL intensity of the HRP-catalyzed luminol-H 2 O 2 reached a steady state for about 30 s at pH 6.5 (Figure 7B).…”

“…Recently, this triple system (luminol/H 2 O 2 /HRP) has been applied by Fang et al. [70] to the determination of H 2 S, in which H 2 S inactivates HRP and thus quenches the system in a quantitative manner with CL (Figure 7A). The CL intensity of the HRP‐catalyzed luminol‐H 2 O 2 reached a steady state for about 30 s at pH 6.5 (Figure 7B).…”

Signal-On and Highly Sensitive Electrochemiluminescence Biosensor for Hydrogen Sulfide in Joint Fluid Based on Silver-Ion-Mediated Base Pairs and Hybridization Chain Reaction

“…An amount of 10 µL of Tris-HClO 4 buffer was dropped on the primer-modified gold electrode, and the working electrode was placed in a tube containing 50 µL of various concentrations of H 2 S standards (using Na 2 S as the source) or joint fluid samples. H 2 S gas could be volatilized from the tube and enriched on the primer-modified gold electrode by headspace enrichment [26,27]. Then, HCR was carried out at 37 • C for 105 min when the electrode was soaked in the solution containing H1 and H2 and Tris-HClO 4 buffer.…”