Chemically induced Salmonella enteritidis ghosts as a novel vaccine candidate against virulent challenge in a rat model

Vinod, Nagarajan; Oh, Sung Jong; Kim, Seong-Dae; Choi, Chang Won; Kim, Sei Chang; Jung, Cheong Hwan

doi:10.1016/j.vaccine.2014.03.090

Cited by 38 publications

(52 citation statements)

References 34 publications

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“…In our study, the use of SEGs Salmonella vaccine for the protection of poultry against infection, in agreement with previous report [31]. On the other hand, the S. enteritidis could be reisolated from vaccinated challenged chickens with SEGs by a lower ratio than from commercial inactivated Salmonella vaccine from the heart blood, liver, spleen, and cecal junction on the 4 th week postchallenge and control unvaccinated birds (Table 6).…”

Section: Resultssupporting

confidence: 92%

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

El-Safty

Mahmoud

Zaki

et al. 2017

Asian J Pharm Clin Res

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Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine). Results:Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH. Conclusion:It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

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Section: Resultssupporting

confidence: 92%

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

El-Safty

Mahmoud

Zaki

et al. 2017

Asian J Pharm Clin Res

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“…While the chemically treated cells show weaker protein bands. This is due to the lack of cytoplasmic contents in BGs cells [18]. In addition, the complete degradation of bacterial genomic DNA was determined using agarose gel electrophoresis (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

Klebsiella pneumoniae Ghosts as Vaccine Using Sponge Like Reduced Protocol

Mm¹,

Hussein²,

Abd³

et al. 2017

Cell Mol Med

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“…Considering the alkali effect on DNA, our data indicates that NaOH causes genomic DNA to be cleaved into small fragments which can be expelled completely from the VPGs. Previous studies also showed that the genomic DNA of a Gram-negative bacterium [16] and a…”

Section: Determination Of Dna-free Vpgs By Agarose Gel Electrophoresimentioning

confidence: 92%

“…The resultant LPS was antigenically poor [35,36] or deficient [37]. However, our previous studies demonstrated that immunization with the NaOH-induced BGs generated from a Gram-negative bacterium and a Gram-positive bacterium, respectively, induces effective immune responses and provides good protection against virulent challenge [16,17]. It suggests that the NaOH treatment does not affect the immunogenicity of VPGs or the potential as a vaccine candidate.…”

Section: Comparison Of Cytotoxicity Test For Vpgsmentioning

confidence: 99%

“…Alternatively, Amara et al [15] demonstrated the protocol for Escherichia coli BGs preparation using the minimum inhibition concentration (MIC) and the minimum growth concentration (MCG) of various chemicals. Recently, sodium hydroxide (NaOH)-induced BGs were generated from a Gram-negative bacterium (Salmonella enteriditis) [16] and a Gram-positive bacterium (Staphylococcus aureus) [17]. Nevertheless, it has never been characterized why NaOH was chosen as the best chemical to produce BGs.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced <em>Vibrio parahaemolyticus </em>Ghosts (VPGs)

Park¹,

Oh²,

Vinod³

et al. 2016

Preprint

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Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically induced lysis and the method is based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid and sulfuric acid. The MIC values of the respective chemicals were 3.125, 6.25, < 50.0, 25.0, 6.25, 1.56 and 0.781 mg/ml. Except boric acid, the lysis efficiency was reached more than 99.99% at 5 min after treatment of all chemicals. Among those chemicals, NaOH-induced VPGs showed completely DNA-free that was confirmed by quantitative real-time PCR. Besides, lipopolysaccharides (LPS) extracted from the NaOH-induced VPGs showed no distinctive band on SDS-PAGE gel after silver staining. On the other hand, LPS extracted from wild-type bacterial cells as well as the organic acids-induced VPGs showed triple major bands and LPS extracted from the inorganic acids-induced VPGs showed double bands. It suggests that some surface structures in LPS of the NaOH-induced VPGs may be lost, weakened or modified by the MIC of NaOH. Nevertheless, Limulus amoebocyte lysate assay revealed that there is no significant difference in endotoxic activity between the NaOH-induced VPGs and wild-type bacterial cells. Macrophages exposed to the NaOH-induced VPGs at 0.5 × 10 6 CFU/mL showed cell viability of 97.9%, however the MIC of NaOH did not reduce the cytotoxic effect of wild-type bacterial cells. Like Escherichia coli LPS, the NaOH-induced VPGs are an excellent activator of pro-inflammatory cytokines (IL-1β and iNOS), anti-inflammatory cytokine (IL-10) and dual activities (IL-6) in the stimulated macrophage cells. On the other hand, the induction of TNF-α mRNA was remarkable in the macrophages exposed with wild-type cells. Scanning electron microscopy showed the formation of trans-membrane lysis tunnel structures in the NaOH-induced VPGs. SDS-PAGE and agarose gel electrophoresis also confirmed that cytoplasmic proteins and genomic DNA released from the VPGs to culture medium through the lysis tunnel structures. Taken together, all these results indicated that the NaOH-induced VPGs show the potency of safe, economical and effective inactivated bacterial vaccine candidate.

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Chemically induced Salmonella enteritidis ghosts as a novel vaccine candidate against virulent challenge in a rat model

Cited by 38 publications

References 34 publications

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

Klebsiella pneumoniae Ghosts as Vaccine Using Sponge Like Reduced Protocol

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced <em>Vibrio parahaemolyticus </em>Ghosts (VPGs)

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