Chemical War in Marine Animal Forests: Natural Products and Chemical Interactions

Ávila, Conxita

doi:10.1007/978-3-030-57054-5_9

Cited by 3 publications

(14 citation statements)

References 359 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteases and their inhibitors are known to be secreted outside the cell and regulate multiple cellular functions that might be in uencing the surrounding microenvironment. In the vast marine habitats, where the bacteria are either free living or living in symbiosis with plant or animal hosts, chemical cues and interactions play a crucial role 41 . Just like the visual or acoustic signals in higher animals 42,43 , chemical signals are used by bacteria for communication 44 .…”

Section: Discussionmentioning

confidence: 99%

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Shintre

Deshpande

Patwardhan

et al. 2022

Preprint

View full text Add to dashboard Cite

Streptomyces have long been studied for their defense metabolites and Protease Inhibitors (PIs) they secrete for mediating biotic interactions. Current study focuses on diversity of extracellular PIs obtained from four marine Streptomyces species isolated from intertidal regions of West Coast of India. Streptomyces PIs showed strong inhibition of subtilisin followed by trypsin and chymotrypsin. Using shotgun proteomics approach, we identified 15 Streptomyces PIs belonging to 4 PI families namely Streptomyces subtilisin inhibitor (SSI), Potato peptidase inhibitor, Ovomucoid and Serpin. This is a first report of identification of Ovomucoid and Potato peptidase inhibitor families from Streptomyces. Amongst the 15 PIs, 12 were SSIs with 20-75% sequence similarity and variations in the conserved 73rd aa residue. Moreover, 10 SSI isoforms were co-expressed in a single species S. longispororuber. In-silico and in-vitro assays with proteases from microbial, bovine and insect sources suggested that the Streptomyces PIs had a broad-spectrum inhibitory activity. In co-culture of Streptomyces with other protease producing bacteria, cellular secretions differentially affected growth, sporulation and/or pigment production. Present study elucidates the diversity and plasticity of extracellular PIs from marine Streptomyces spp. Further, their role in chemical ecology of the bacterial communities as well as their potential applications in therapeutics are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Shintre

Deshpande

Patwardhan

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Our results indicate that all the marine molecules analyzed here appear to have a certain toxic propensity, albeit low in most cases. These results can be explained by the fact that these marine molecules originate from marine organisms that use them as chemical defenses against potential predators and competitors [47]. A similar example is the wellknown Paclitaxel, Taxol by trade name, a chemical defense alkaloid with toxic effects on mammalian cells that has become a highly used anticancer chemotherapy drug [48].…”

Section: Toxicity Predictionmentioning

confidence: 99%

“…Only those relations that come from the well-known database DrugBank [32] were selected. Later on, the 3D structure of those drugs selected were downloaded from PubChem compound database [47].…”

Section: Drug Likeness Evaluationmentioning

confidence: 99%

Computer-Aided Drug Design (CADD) to De-Orphanize Marine Molecules: Finding Potential Therapeutic Agents for Neurodegenerative and Cardiovascular Diseases

Llorach-Parés

Nonell-Canals²,

Ávila

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

Computer-aided drug design (CADD) techniques allow the identification of compounds capable of modulating protein functions in pathogenesis-related pathways, which is a promising line on drug discovery. Marine natural products (MNPs) are considered a rich source of bioactive compounds, as the oceans are home to much of the planet’s biodiversity. Biodiversity is directly related to chemodiversity, which can inspire new drug discoveries. Therefore, natural products (NPs) in general, and MNPs in particular, have been used for decades as a source of inspiration for the design of new drugs. However, NPs present both opportunities and challenges. These difficulties can be technical, such as the need to dive or trawl to collect the organisms possessing the compounds, or biological, due to their particular marine habitats and the fact that they can be uncultivable in the laboratory. For all these difficulties, the contributions of CADD can play a very relevant role in simplifying their study, since, for example, no biological sample is needed to carry out an in-silico analysis. Therefore, the amount of natural product that needs to be used in the entire preclinical and clinical study is significantly reduced. Here, we exemplify how this combination between CADD and MNPs can help unlock their therapeutic potential. In this study, using a set of marine invertebrate molecules, we elucidate their possible molecular targets and associated therapeutic potential, establishing a pipeline that can be replicated in future studies.

show abstract

“…In these habitats, strong predation pressure and huge competition for resources exist [1,3,[6][7][8][9][10], with interactions occurring there being crucial for structuring Antarctic communities [3,4,8]. Consequently, many effective defensive mechanisms have appeared along evolution to ensure species survival [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Defensive strategies in the Southern Ocean include the use of chemicals (marine natural products or MNPs) to obtain protection against potential predators, among many other roles [8,13,[18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, many effective defensive mechanisms have appeared along evolution to ensure species survival [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Defensive strategies in the Southern Ocean include the use of chemicals (marine natural products or MNPs) to obtain protection against potential predators, among many other roles [8,13,[18][19][20][21][22][23][24][25][26]. Indeed, Antarctic macroorganisms do present a wide array of bioactive molecules that can be used in situ by the organisms but also may potentially be useful from a pharmacological perspective [23,24,26,27].…”

Section: Introductionmentioning

confidence: 99%

Would Antarctic Marine Benthos Survive Alien Species Invasions? What Chemical Ecology May Tell Us

et al. 2022

View full text Add to dashboard Cite

Many Antarctic marine benthic macroinvertebrates are chemically protected against predation by marine natural products of different types. Antarctic potential predators mostly include sea stars (macropredators) and amphipod crustaceans (micropredators) living in the same areas (sympatric). Recently, alien species (allopatric) have been reported to reach the Antarctic coasts, while deep-water crabs are suggested to be more often present in shallower waters. We decided to investigate the effect of the chemical defenses of 29 representative Antarctic marine benthic macroinvertebrates from seven different phyla against predation by using non-native allopatric generalist predators as a proxy for potential alien species. The Antarctic species tested included 14 Porifera, two Cnidaria, two Annelida, one Nemertea, two Bryozooa, three Echinodermata, and five Chordata (Tunicata). Most of these Antarctic marine benthic macroinvertebrates were chemically protected against an allopatric generalist amphipod but not against an allopatric generalist crab from temperate waters. Therefore, both a possible recolonization of large crabs from deep waters or an invasion of non-native generalist crab species could potentially alter the fundamental nature of these communities forever since chemical defenses would not be effective against them. This, together with the increasing temperatures that elevate the probability of alien species surviving, is a huge threat to Antarctic marine benthos.

show abstract

Chemical War in Marine Animal Forests: Natural Products and Chemical Interactions

Cited by 3 publications

References 359 publications

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Computer-Aided Drug Design (CADD) to De-Orphanize Marine Molecules: Finding Potential Therapeutic Agents for Neurodegenerative and Cardiovascular Diseases

Would Antarctic Marine Benthos Survive Alien Species Invasions? What Chemical Ecology May Tell Us

Contact Info

Product

Resources

About