Chemical profiling of infusions and decoctions of Helichrysum italicum subsp. picardii by UHPLC-PDA-MS and in vitro biological activities comparatively with green tea (Camellia sinensis) and rooibos tisane (Aspalathus linearis)

Abstract

Several medicinal plants are currently used by the food industry as functional additives, for example botanical extracts in herbal drinks. Moreover, the scientific community has recently begun focusing on halophytes as sources of functional beverages. Helichrysum italicum subsp. picardii (everlasting) is an aromatic halophyte common in southern Europe frequently used as spice and in traditional medicine. In this context, this work explored for the first time H. italicum subsp. picardii as a potential source of innovative herbal beverages with potential health promoting properties. For that purpose, infusions and decoctions were prepared from roots, vegetative aerial-organs (stems and leaves) and flowers and evaluated for in vitro antioxidant and anti-diabetic activities. Samples were also assessed for toxicity in different mammalian cell lines and chemically characterized by spectrophotometric methods and ultra-high performance liquid chromatography-photodiode array-mass-spectrometry (UHPLC-PDA-MS). Results were expressed relating to 'a cup-of-tea' and compared with those obtained with green tea (Camellia sinensis) and rooibos tisane (Aspalathus linearis). Tisanes from the everlasting's above-ground organs, particularly flowers, have high polyphenolic content and several phenolics were identified; the main compounds were chlorogenic and quinic acids, dicaffeoylquinic-acid isomers and gnaphaliin-A. The antioxidant activity of beverages from the everlasting's above-ground organs matched or surpassed that of green tea and rooibos. Its anti-diabetic activity was moderate and toxicity low. Overall, our results suggest that the everlasting is a potential source of innovative and functional herbal beverages.

Health promoting potential of herbal teas and tinctures from Artemisia campestris subsp. maritima: from traditional remedies to prospective products

“… 34 , previously validated by those authors for quantitation of phenolic constituents in apple cultivars, and is fully detailed in Pereira et al . 5 including performance characteristics, quantification procedures and compound tentative identification specifics. The aim was to (tentatively) identify phytochemical constituents in the dune wormwood extracts, getting an estimate of their concentrations and/or relative abundances when no reference standards were available.…”

“…Helichrysum italicum subsp. picardii 5 , Crithmum maritimum 6 , Limonium algarvense 7 ), or as raw material for pharmaceutical and other related industries (e.g. Lithrum salicaria 8 , Polygonum maritimum 9 ).…”

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

“…The LC-MS data of the negative ionization mode, identified a caffeoylquinic acid derivative as a possible constituent in the crude methanolic extract (Table 2, Figure S2 and Figure S3). The identified mass fragment m/z 515.1223 making up the peak at 3.71 min ( Figure S4-Supplementary Data) was of particular interest since the possible compound hits included some caffeoylquinic acid derivatives which have been previously identified in other Helichrysum species, including Helichrysum italicum, Helichrysum populifolium, and Helichrysum stoechas (Barroso et al, 2014;Heyman et al, 2015;Maksimovic et al, 2017;Pereira et al, 2017). The mass fragments m/z 353.1017 and 191.0762 corresponded to previous data on Helichrysum obconicum reported by Gouveia and Castilho (2011) corresponding to some caffeoyl-quinic acid derivatives.…”

Extraction of Natural Fragrance Ingredients: History Overview and Future Trends

“…Supercritical fluids constitute an opportunity to produce eco‐friendly extracts free of all traces of solvent, while being safe for the workers and the environment. Considering the whole process, and its global environmental impact, supercritical fluid extraction constitutes thereby a good alternative to traditional solvent extraction and steam distillation at lower operational cost, contrary to the general opinion (actual high initial investment but cost effective in the long run considering the whole process) . The main constraints limiting the implementation of SFE at the industrial scale is the difficulty to build units appropriate to resist to very high pressure (up to 1000 bar)…”

“…Considering the whole process, and its global environmental impact, supercritical fluid extraction constitutes thereby a good alternative to traditional solvent extraction and steam distillation at lower operational cost, contrary to the general opinion (actual high initial investment but cost effective in the long run considering the whole process). [64] The main constraints limiting the implementation of SFE at the industrial scale is the difficulty to build units appropriate to resist to very high pressure (up to 1000 bar). [67] At critical conditions for pressure (above 74 bars) and temperature (above 31°C), CO 2 has the ability to behave like a fluid.…”