2016
DOI: 10.5935/0103-5053.20160189 View full text |Buy / Rent full text
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Abstract: The chemical profiles of four ginseng roots samples from three species of ginseng (Panax quinquefolius, Panax ginseng and Panax notoginseng) and two commercial ginseng products containing P. quinquefolius and red P. ginseng were compared using ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC/QTOF-MS). Principal component analysis allowed a holistic approach in showing distinct chemical differences between the three ginseng species and correct classification… Show more

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“…With advances in chromatography technologies in the past decade, ultra-high performance liquid chromatography (UHPLC) has enabled rapid separation of phenolics with much reduced time and cost [ 52 ]. UHPLC or UPLC is a chromatographic technique that is commercially available since 2004, and its applications have been rising steadily also for the qualification and quantification of the major phenolic compounds of several Lamiaceae herbs and spices [ 85 , 86 , 87 ]. The capability of higher pressure that ranges up to 15,000 psi (1035 bar) [ 86 , 88 ] and smaller particle size (potentially lower than 2 μm) [ 55 , 86 , 88 ], result in more rapid [ 55 , 86 , 88 , 89 ] effective [ 86 ], and sensitive separation of analytes [ 88 ].…”
Section: Chromatographic Techniques With Ultraviolet/visible (Uv/vmentioning
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“…With advances in chromatography technologies in the past decade, ultra-high performance liquid chromatography (UHPLC) has enabled rapid separation of phenolics with much reduced time and cost [ 52 ]. UHPLC or UPLC is a chromatographic technique that is commercially available since 2004, and its applications have been rising steadily also for the qualification and quantification of the major phenolic compounds of several Lamiaceae herbs and spices [ 85 , 86 , 87 ]. The capability of higher pressure that ranges up to 15,000 psi (1035 bar) [ 86 , 88 ] and smaller particle size (potentially lower than 2 μm) [ 55 , 86 , 88 ], result in more rapid [ 55 , 86 , 88 , 89 ] effective [ 86 ], and sensitive separation of analytes [ 88 ].…”
Section: Chromatographic Techniques With Ultraviolet/visible (Uv/vmentioning
“…Two types of mobile phases were used which were A (water in 0.1 % formic acid) and B (acetonitrile in 0.1 % formic acid) phases. The methods for operating the UPLC was adapted from Yuk et al. (2016) study with some modifications.…”
Section: Methodsmentioning
“…Among the various available approaches, liquid chromatography (LC)–mass spectrometry (MS) is a widely used analytical technique with high separation and detection sensitivity to diverse chemical components. Meanwhile, methods based on rapid chromatographic separation and high-resolution MS are being increasingly employed and ultra-performance LC (UPLC) has become a prevalent analytical platform in chemical profiling filed as it employs higher pressure of 12,000–15,000 p.s.i compared to high-performance LC and generates smaller particle size of approximately 2 μm which accommodates more rapid and efficient separation for complex natural product mixtures ( Yuk et al., 2016 ).…”
Section: Introductionmentioning
“…However, no analytical method has been reported for the chemical analysis of caffeoylquinic acid derivatives, flavonoids, and triterpene glycosides in a single analytical method. Due to the higher sales of commercial natural products in the market, there have been increasing cases of intentional adulteration with different species to lower the cost [10]. To ensure quality control of ci-wu-jia or Siberian ginseng tea in the market, it is important to develop an analytical method for the characterization of these types of major constituents in E. senticosus leaves.…”
Section: Introductionmentioning