Chemical cytometry on a picoliter-scale integrated microfluidic chip

Monolithic integration of fine cylindrical glass microcapillaries on silicon for electrophoretic separation of biomolecules

“…We are currently extending our work towards the utilization of these microcapillaries for analyzing chemical content of individual cells (chemical cytometry). 41 …”

Microfluidic single-cell analysis of intracellular compounds

“…Deformable membranes realized in PDMS devices (Unger et al 2000) are insensitive to almost all physico-chemical properties of the microdevice (Squires & Quake 2005); however, valves are sensitive to high pumping pressures. This principle has been applied to a variety of other analytical microfluidic devices, some of them for singlecell analysis and are included in this review (Hong et al 2004;Wu et al 2004;Marcus et al 2006;Huang et al 2007;Marcy et al 2007a,b;Zhong et al 2008).…”

“…The released amino acids were derivatized with NDA and separated by micellar electrokinetic chromatography ( Wu et al 2004), thus allowing the identification of individual amino acids. The Zare group further presented the first example to count proteins in single cells ).…”

Basic Technologies for Droplet Microfluidics

“…Other advantages of droplet-based [76] microfluidic system include precisely controllable reaction time inside a droplet by adjusting the length of channel and the flow rates of fluids. The availability of a wide range of technologies for flexible generation and manipulation of droplets has enabled the applications of droplet microfluidics in a wide variety of fields, from chemical reactions [9,79] and protein crystallization [10,11] to material synthesis [12,13,[80][81][82][83], single cell analysis [84][85][86][87][88][89][90][91], DNA amplification [16], protein engineering [62,92], and high-throughput screening technologies [17,93]. It is not possible to cover all application areas in this review.…”