Chemical Constituents ofClusia nemorosaFruits and Evaluation of Antioxidant and Antimicrobial Activity

Abstract

Background: Clusia nemorosa, popularly known as pororoca, is used in folk medicine to treat inflammation. Objective: The current study was conducted to isolate and identify bioactive compounds from C. nemorosa fruits and to investigate their antimicrobial and antioxidant activities. Method: The isolation and structural elucidation of the substances were carried out by usual chromatographic techniques and spectroscopic methods, respectively. The antioxidant activity of extracts of C. nemorosa fruits was measured by DPPH assay and antimicrobial activity was evaluated against the microorganisms Staphylococcus aureus, Candida albicans, Cryptococcus neoformans and Rhizopus oryzae. Results: The chemical investigation of the fruit extract of C. nemorosa led to the identification of two phenolic acids, protocatechuic acid (1) and coumaric acid (6), a flavonoid apigenin (7), glycosyl-β-sitosterol (4), glycosyl-stigmasterol (5), citric acid (3), and the trimethyl citrate ester (2). The fraction in AcOET showed the best scavenging activity of the DPPH radical, with IC50 23.50±1.7 µg. mL-1. The extracts were inactive against the tested microorganisms up to 2500 µg. mL-1. Conclusion: With the exception of the steroid glycosyl-β-sitosterol, the substances are being described for the first time in the species and, in addition, we report the promising free radical scavenging activity showing its potential in the treatment of diseases related to oxidative stress.