Chemical composition of Lycium europaeum fruit oil obtained by supercritical CO 2 extraction and evaluation of its antioxidant activity, cytotoxicity and cell absorption

Rosa, Antonella; Maxia, Andrea; Putzu, Danilo; Atzeri, Angela; Era, Benedetta; Fais, Antonella; Sanna, Cinzia; Piras, Alessandra

doi:10.1016/j.foodchem.2017.03.019

Cited by 35 publications

(56 citation statements)

References 46 publications

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“…Dried saponifiable fractions, with fatty acids and conjugated diene fatty acid hydroperoxides (HP), were dissolved in acetonitrile/0.14% acetic acid (v/v) and then analyzed by high‐performance liquid chromatography (HPLC). A portion of dried fatty acids after saponification was methylated with methanolic HCl (3 N) (room temperature) as previously reported and FAME were analyzed by gas chromatography (GC).…”

Section: Methodsmentioning

confidence: 99%

“…FAME were measured on a gas chromatograph HP‐6890 (Hewlett‐Packard, Palo Alto, USA) with a flame ionization detector (GC‐FID) and equipped with a cyanopropyl methyl‐polysiloxane HP‐23 FAME column as previously reported . FAME were identified with standard compounds and quantified as a percentage of the total amount of FA (g%).…”

Section: Methodsmentioning

confidence: 99%

“…The unsaturated FA (UFA) composition and HP levels of SFE and SoxE seed oils were obtained with a 1100 HPLC‐DAD system (Agilent Technologies, Palo Alto, CA) as previously reported . UFA, detected at 200 nm, and HP, detected at 234 nm, were eluted with CH 3 CN/H 2 O/CH 3 COOH (75/25/0.12, v/v/v) as mobile phase at a flow rate of 2.3 mL min −1 , using an Agilent Technologies XDB‐C 18 Eclipse column . UFA were identified using standard compounds (linear calibration curves, correlation coefficients > 0.995) and the conventional UV spectra; chromatogram data were recorded and integrated with an Agilent OpenLAB Chromatography data system …”

Section: Methodsmentioning

confidence: 99%

“…Aliquots of SFE and SoxE seed oils were dissolved in EtOH (2 mg mL −1 ). Analyses of TAG were carried out with a 1100 HPLC system equipped with 1260 Infinity ELSD; separation was performed with an Inertsil ODS‐2 column, and MeOH as the mobile phase, at a flow rate of 2 mL min −1 . Standard solutions of TAG (LnLnLn, LLL, LLO, LLP, OOL, OOO, and PPP) were prepared in MeOH.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, several studies have reported the use of the CO 2 ‐SFE extraction for the recovery of fixed oils from bergamot ( Citrus bergamia Risso), yuza ( C. ichangensis × C. reticulata ), and yuzu ( C. junos ) seeds . CO 2 ‐SFE is an alternative, environmentally benign, separation technique, widely used to obtain fixed oils from seeds …”

Section: Introductionmentioning

confidence: 99%

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Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Rosa

Era

Masala

et al. 2019

Euro J Lipid Sci & Tech

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Seeds of Citrus plants (Rutaceae), an major agro‐industrial waste, are a potential source of valuable by‐products. The chemical composition, the antioxidant profile, and the biological/nutritional properties of fixed oils (SFE oils) obtained by supercritical CO2 extraction (at 300 bar and 40 °C) from seeds of mandarin (Citrus reticulata), lemon (Citrus limon), and grapefruit (Citrus paradisi), discarded by a local agro‐alimentary industry are studied. The yields and compositions of SFE oils are compared to oils obtained by n‐hexane extraction of Citrus seeds in a Soxhlet apparatus (SoxE oils). Linoleic (35–42%), oleic (22–28%), palmitic (21–25%), α‐linolenic (4–10%), and stearic (4–7%) are the main oil fatty acids, while monolinolein and dilinolein derivatives represent the main triacylglycerols in SFE oils, determined by GC‐FID and HPLC‐DAD/ELSD techniques. Citrus SFE oils show lower yields than SoxE oils but similar chemical profiles. Principal components analysis applied to fatty acid and triacylglycerol data indicates that the oils from different seeds are tightly clustered. Moreover, SFE oils show radical scavenging activities (ABTS assays), an inhibitory effect on the growth of murine B16F10 melanoma cells and on the mushroom tyrosinase activity. Citrus SFE oils and de‐coated seeds are also evaluated for their attractant effect on the Mediterranean fruit fly Ceratitis capitata by behavioral assays. The results qualify Citrus SFE oils as a potential natural, environmentally‐friendly, resource for food/pharmaceutical applications. Practical Applications: The environmentally‐friendly supercritical CO2 extraction of waste lemon, mandarin, and grapefruit seeds gives edible fixed oils a richness in essential fatty acids, suitable for human nutrition and the preparation of nutraceuticals and pharmaceuticals. Edible fixed oils obtained from lemon, mandarin, and grapefruit seeds by SFE‐CO2 extraction show high levels of essential fatty acids, antioxidant, anticancer, and antityrosinase activity.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Rosa

Era

Masala

et al. 2019

Euro J Lipid Sci & Tech

Self Cite

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Evaluation of the attractant effect and lipid profile modulation of natural fixed oils on the medfly Ceratitis capitata (Wiedemann)

Rosa

Piras

Carta

et al. 2018

Arch Insect Biochem Physiol

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The Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824; Diptera: Tephritidae), is a polyphagous pest in horticulture, mainly targeting Citrus fruits. Natural essential and fixed oils are currently under investigation for their broad-spectrum in pest control. To gain better knowledge about medfly behavior and biochemistry, we examined with behavioral and biochemical assays, the effects on C. capitata from six natural fixed oils obtained from vegetable (five) or animal (one) matrices using the ecofriendly supercritical CO 2 extraction. Oils were obtained at 250/300 bar and 40°C from the seeds of Laurus nobilis and Citrus paradisi, the fruits of Myristica fragrans and Pistacia terebinthus, wheat germ, and mullet roes (marine oil). Behavioral experiments were performed by means of twochoice tests to analyze the oil attractant effect compared with control (water or standard diet). The fatty acid composition of oils and the total lipid and fatty acid profile of medflies were characterized by chromatographic techniques. Behavioral bioassays showed that fixed oil obtained from M. fragrans (nutmeg butter) was more attractive than other oils. Medflies fed (24 hr) on marine oil showed significant changes in the total lipid and fatty acid profile induced by oil ingestion without toxic effects. However, 56% mortality was observed in insects fed on M. fragrans oil and no biochemical changes ascribable to oil ingestion were detected in the medflies that survived. Our results advance Arch. Insect Biochem. Physiol. 2018;99:e21508. wileyonlinelibrary.com/journal/arch

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Recent advances of green pretreatment techniques for quality control of natural products

Sun

Yan

et al. 2020

Electrophoresis

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A few advancing technologies for natural product analysis have been widely proposed, which focus on decreasing energy consumption and developing an environmentally sustainable manner. These green sample pretreatment and analysis methods following the green Analytical Chemistry (GAC) criteria have the advantage of improving the strategy of chemical analyses, promoting sustainable development to analytical laboratories, and reducing the negative effects of analysis experiments on the environment. A few minimized extraction methodologies have been proposed for replacing the traditional methods in the quality evaluation of natural products, mainly including solid‐phase microextraction (SPME) and liquid phase microextraction (LPME). These procedures not only have no need for large numbers of samples and toxic reagent, but also spend a small amount of extraction and analytical time. This overview aims to list out the main green strategies on the application of quality evaluation and control for natural products in the past 3 years.

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Chemical composition of Lycium europaeum fruit oil obtained by supercritical CO 2 extraction and evaluation of its antioxidant activity, cytotoxicity and cell absorption

Cited by 35 publications

References 46 publications

Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Evaluation of the attractant effect and lipid profile modulation of natural fixed oils on the medfly Ceratitis capitata (Wiedemann)

Recent advances of green pretreatment techniques for quality control of natural products

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Chemical composition of Lycium europaeum fruit oil obtained by supercritical CO 2 extraction and evaluation of its antioxidant activity, cytotoxicity and cell absorption

Cited by 35 publications

References 46 publications

Supercritical CO2 Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Supercritical CO2 Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Evaluation of the attractant effect and lipid profile modulation of natural fixed oils on the medfly Ceratitis capitata (Wiedemann)

Recent advances of green pretreatment techniques for quality control of natural products

Contact Info

Product

Resources

About

Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils

Supercritical CO₂ Extraction of Waste Citrus Seeds: Chemical Composition, Nutritional and Biological Properties of Edible Fixed Oils