Chelate-free metal ion binding and heat-induced radiolabeling of iron oxide nanoparticles

Boros, Eszter; Bowen, Alice M.; Josephson, Lee; Vasdev, Neil; Holland, Jason P.

doi:10.1039/c4sc02778g

Cited by 109 publications

(156 citation statements)

References 49 publications

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“…Finally, the technique is likely applicable to other nanoparticles, for instance by incorporation of lanthanides using heat-induced labeling. 29 Delivered by Ingenta to: Nanyang Technological …”

Section: Resultsmentioning

confidence: 99%

“…11 12 28 Besides incorporating europium (or other lanthanides) during synthesis as demonstrated here, a new report suggests that it is possible to incorporate lanthanides into other nanoparticles, e.g., the clinically approved ferumoxytol, by using heat-induced labeling after synthesis. 29 After IV administration, Eu-VSOP behaved like VSOP, accumulating in the liver and in atherosclerotic plaques of ApoE −/− mice. 11 Due to the high sensitivity of LA-ICP-MS for europium and the minimal natural europium background in tissue samples, this accumulation was sufficient for sensitive and specific detection of Eu-VSOP in atherosclerotic plaques of ApoE −/− mice.…”

Section: Discussionmentioning

confidence: 99%

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LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

Scharlach¹,

Müller²,

Wagner³

et al. 2016

j biomed nanotechnol

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The development of iron oxide nanoparticles for biomedical applications requires accurate histological evaluation. Prussian blue iron staining is widely used but may be unspecific when tissues contain substantial endogenous iron. Here we tested whether microscopy by laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) is sensitive enough to analyze accumulation of very small iron oxide particles (VSOP) doped with europium in tissue sections. For synthesis of VSOP, a fraction of Fe3+ (5 wt%) was replaced by Eu3+, resulting in particles with 0.66 mol% europium relative to iron (Eu-VSOP) but with otherwise similar properties as VSOP. Eu-VSOP or VSOP was intravenously injected into ApoE-/- mice on Western cholesterol diet and accumulated in atherosclerotic plaques of these animals. Prussian blue staining was positive for ApoE-/- mice with particle injection but also for controls. LA-ICP-MS microscopy resulted in sensitive and specific detection of the europium of Eu-VSOP in liver and atherosclerotic plaques. Furthermore, calibration with Eu-VSOP allowed calculation of iron and particle concentrations in tissue sections. The combination of europium-doped iron oxide particles and LA-ICP-MS microscopy provides a new tool for specific and quantitative analysis of particle distribution at the tissue level and allows correlation with other elements such as endogenous iron.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

Scharlach¹,

Müller²,

Wagner³

et al. 2016

j biomed nanotechnol

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“…Inorganic bases are often used to adjust the pH for radiolabeling reactions by many research groups. 26,27 Then, 0.1 M NaOH was first used to adjust the pH of the reaction mixture to 8.0. However, the use of NaOH resulted in the pH becoming excessively high and causing precipitation of Co(OH) 2 in the reaction mixture.…”

Section: Resultsmentioning

confidence: 99%

“…Two types of sterically stabilized SPIONs are used in this study: 10 nm diameter (small core) and 25 10,11 This potentially offers better negative MRI contrast agents than all commercially and clinically available samples of similar core sizes. Ligand-free radiolabeling, which was developed recently for commercially available iron oxide nanoparticles with PET radiotracers, 26,27 is applied to incorporate metal ions of Co, Ga, and Cu as models for PET/SPECT radiotracers. Radiolabeling kinetics, stability, and cytotoxicity of the radiolabeled SPIONs are also investigated.…”

mentioning

confidence: 99%

Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles

Pham¹,

Lengkeek²,

Greguric³

et al. 2017

IJN

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Physiologically stable multimodality imaging probes for positron emission tomography/single-photon emission computed tomography (PET/SPECT)-magnetic resonance imaging (MRI) were synthesized using the superparamagnetic maghemite iron oxide (γ-Fe 2 O 3 ) nanoparticles (SPIONs). The SPIONs were sterically stabilized with a finely tuned mixture of diblock copolymers with either methoxypolyethylene glycol (MPEG) or primary amine NH 2 end groups. The radioisotope for PET or SPECT imaging was incorporated with the SPIONs at high temperature. 57 Co 2+ ions with a long half-life of 270.9 days were used as a model for the radiotracer to study the kinetics of radiolabeling, characterization, and the stability of the radiolabeled SPIONs. Radioactive 67 Ga 3+ and Cu 2+ -labeled SPIONs were also produced successfully using the optimized conditions from the 57 Co 2+ -labeling process. No free radioisotopes were detected in the aqueous phase for the radiolabeled SPIONs 1 week after dispersion in phosphate-buffered saline (PBS). All labeled SPIONs were not only well dispersed and stable under physiological conditions but also noncytotoxic in vitro. The ability to design and produce physiologically stable radiolabeled magnetic nanoparticles with a finely controlled number of functionalizable end groups on the SPIONs enables the generation of a desirable and biologically compatible multimodality PET/SPECT-MRI agent on a single T2 contrast MRI probe.

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“…PET/CT imaging studies were performed, confirming the ability of these nanoplatforms to be radiolabeled with other radioisotopes (i.e. 64 Cu 2+ , 89 Zr 4+ , 111 In 3+ ) under the same radiolabeling conditions [35]. In another study, ferumoxytol was linked to the desferrioxamine chelate (DFO) and radiolabeled with 89 Zr, in order to acquire a tool that could combine PET and MR imaging for the enhanced detection of lymphatic drainage [36].…”

Section: Zirconium-89mentioning

confidence: 88%

Radiolabeled Nanoparticles in Nuclear Oncology

2018

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A B S T R A C TDuring recent years, a plethora of pioneering radiolabeled nanoparticles have grown to be an integral part of nuclear medicine as theranostic tools. Herein, we focus on the most representative examples of nanoparticles of the past decade, which have been investigated in conjunction with radioisotopes aiming to serve as drug delivery or imaging agents. The present review highlights the key attributes of each nanosystem and the following classification of radiolabeled nanovehicles is based on increasing mass number (A) of radioisotopic elements.

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Chelate-free metal ion binding and heat-induced radiolabeling of iron oxide nanoparticles

Abstract: Holland and co-workers report details of a novel chelate-free reaction for radiolabeling of pre-fabricated nanoparticles using different radionuclides.

Cited by 109 publications

References 49 publications

LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

LA-ICP-MS Allows Quantitative Microscopy of Europium-Doped Iron Oxide Nanoparticles and is a Possible Alternative to Ambiguous Prussian Blue Iron Staining

Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles

Radiolabeled Nanoparticles in Nuclear Oncology

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