Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family

Estrada-Gómez, Sebastián; Gomez-Rave, Lyz; Muñoz, Leidy Johana Vargas; Meijden, Arie van der

doi:10.1016/j.toxicon.2017.02.007

Cited by 17 publications

(10 citation statements)

References 57 publications

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“…Interestingly, our bioactivity assay results suggest that the venom of P. verdolaga has the ability to preferentially modulate Ca V channels over Na V channels, although more detailed experiments are required to explore these observations further at the single peptide level using ion channel electrophysiology assays. These findings are in agreement with previous reports of P. verdolaga venom that described the presence of peptides with high identity with Ca V channel modulators from other Theraphosids [17,18].…”

Section: Discussionsupporting

confidence: 93%

“…P. verdolaga is a recently described Theraphosidae spider distributed mainly in the region of Antioquia (Colombia) [29]. MS/MS and transcriptomics analyses of the venom-gland content showed that P. verdolaga venom is not only composed of theraphotoxins, but also by larger proteins, in agreement with what has been previously reported for tarantulas [17].…”

Section: Discussionsupporting

confidence: 81%

“…A previous report from P. verdolaga showed that this venom contains low and high molecular mass proteins (i.e., phospholipase A 2 , sphingomyelinase D, hyaluronidase, Kunitz-type serine protease inhibitors, and compounds similar to lycotoxins) [17]. Several ICK fragments that matched theraphotoxins and affected voltage-gated calcium channels (U2-theraphotoxin-Asp1a and ω-theraphotoxin-Hh1a) were also described in this venom [18].…”

Section: Introductionmentioning

confidence: 84%

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Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Estrada-Gómez

Cardoso

Muñoz

et al. 2019

Toxins

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Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on CaV2.2, CaV3.2, and NaV1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ω-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ω-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 84%

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Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Estrada-Gómez

Cardoso

Muñoz

et al. 2019

Toxins

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“…Defensin is a class of cationic peptides rich in disulfide bonds and widely distributed in fungi, plants and animals, and is also an important part of the defense system [1]. Scorpion defensins have a variety of biological activities, including antiviral activity [2,3], antibacterial activity [4,5], immunomodulatory function [6,7], antitumor action [8,9], and ion channel modulation [10,11]. A scorpion defensin BmKDfsin4 derived from the venom of the scorpion Mesobuthus martensii Karsch was reported to inhibit hepatitis B virus (HBV) replication by our group [12].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus

Cheng

Sun

et al. 2020

Antibiotics

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Hepatitis C virus (HCV) infection is a major worldwide health problem which can cause chronic hepatitis, liver fibrosis and hepatocellular carcinoma (HCC). There is still no vaccine to prevent HCV infection. Currently, the clinical treatment of HCV infection mainly relies on the use of direct-acting antivirals (DAAs) which are expensive and have side effects. Here, BmKDfsin3, a scorpion defensin from the venom of Mesobuthus martensii Karsch, is found to dose-dependently inhibit HCV infection at noncytotoxic concentrations and affect viral attachment and post-entry in HCV life cycle. Further experimental results show that BmKDfsin3 not only suppresses p38 mitogen-activated protein kinase (MAPK) activation of HCV-infected Huh7.5.1 cells, but also inhibits p38 activation of Huh7.5.1 cells stimulated by tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or lipopolysaccharide (LPS). BmKDfsin3 is also revealed to enter into cells. Using an upstream MyD88 dimerization inhibitor ST2345 or kinase IRAK-1/4 inhibitor I, the inhibition of p38 activation represses HCV replication in vitro. Taken together, a scorpion defensin BmKDfsin3 inhibits HCV replication, related to regulated p38 MAPK activation.

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“…There are several scorpion species (n = 24) inhabiting Egypt including the Buthidae scorpion of A. amoreuxi. Previously, the venom of this species exhibited strong anticancer (Salem, Shoukry, Teleb, Abdel-Daim, & Abdel-Rahman, 2016) and antimicrobial (Almaaytah et al, 2012;Estrada-Gómez, Gomez-Rave, Vargas-Muñoz, & van der Meijden, 2017) activities using in vivo and in vitro studies. The present work was conducted to extend our earlier pharmacological study (Salem et al, 2016) through investigating the antinociceptive, antipyretic, and anti-inflammatory effects of the Egyptian scorpion venom A. amoreuxi in both rats and mice animal models.…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive, antiinflammatory, and antipyretic effects induced by the venom of Egyptian scorpion Androctonus amoreuxi

Shoukry

Salem

Teleb

et al. 2020

JoBAZ

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Background Scorpion venom is a very complicated mixture of various peptides/proteins which could induce toxicological and pharmacological responses. This investigation was conducted to evaluate the possible pharmacological properties (analgesic, antipyretic, and antiinflammatory effects) of the Egyptian scorpion venom Androctonus amoreuxi in mice and rats injected intraperitoneally with 1/10 and 1/5 LD50 (0.11 and 0.22 mg/kg for mice; 0.385 and 0.77 mg/kg for rats, respectively). Results The peripheral and central analgesic effect of A. amoreuxi venom was determined using the tests of mice-abdominal writhing and tail immersion of rats, respectively. The antipyretic and antiinflammatory activities were examined using the pyrexia rats model induced by Brewer’s yeast and the paw mice edema induced by carrageenan, respectively. The venom of A. amoreuxi produced significant (p < 0.05) peripheral and central analgesic activity in both animal models. Also, treatment with the scorpion venom showed significant (p < 0.05) dose-independent reduction in pyrexia of rats. More importantly, the venom significantly inhibited mice paw edema induced by carrageenan. Conclusion Accordingly, the present results showed that the venom of this scorpion possesses remarkable pharmacological properties (analgesic, antipyretic, and antiinflammatory activities) on animal models, and might be contain certain peptides responsible for the reported activities.

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Characterizing the biological and biochemical profile of six different scorpion venoms from the Buthidae and Scorpionidae family

Cited by 17 publications

References 57 publications

Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Inhibitory Activity of a Scorpion Defensin BmKDfsin3 against Hepatitis C Virus

Antinociceptive, antiinflammatory, and antipyretic effects induced by the venom of Egyptian scorpion Androctonus amoreuxi

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