Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Tello, Mario; Corsini, Gino; Larrondo, Luis F.; Salas, Loreto; Lobos, Sergio; Vicuña, Rafael

doi:10.1016/s0167-4781(99)00227-4

Cited by 48 publications

(29 citation statements)

References 30 publications

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“…Designated CsA and CsB, they harbored the Csmnp2a and Csmnp2b sequences, respectively. The identity of each PCR product was unambiguously established by direct sequencing and comparison to previously reported sequences [16].…”

Section: Resultsmentioning

confidence: 99%

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Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Tello

2001

FEMS Microbiology Letters

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Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes. ß

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Section: Resultsmentioning

confidence: 99%

“…subvermispora sequences encoding a laccase [14] and four MnPs [15,16] have been reported. The MnP-like sequences designated Csmnp2a and Csmnp2b are 97% identical [16]. This observation, together with the probable dikaryotic nature of C. subvermispora [17], suggests an allelic relationship.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Tello

2001

FEMS Microbiology Letters

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“…In several fungi, a sufficient amount of Mn 2ϩ in the medium is required for MnP expression at the mRNA level (19). Differential regulation of mnp genes by manganese in Pleurotus ostreatus (4), C. subvermispora (18,32), and T. versicolor (6, 10, 12) was previously described. Addi- FIG.…”

Section: Discussionmentioning

confidence: 99%

Saline-Dependent Regulation of Manganese Peroxidase Genes in the Hypersaline-Tolerant White Rot Fungus Phlebia sp. Strain MG-60

Kamei

Daikoku

Tsutsumi

et al. 2008

Appl Environ Microbiol

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Mangroves are trees that grow in saline habitats in the tropics and subtropics. Plants in mangrove forests have developed a set of physiological adaptations in response to frequent tidal inundation. Mangroves and sea grasses provide a natural habitat for marine fungi. Marine fungi are often found on decayed lignocellulosic substrates such as prop roots, pneumatophores, branches, leaves, and driftwood in the intertidal region of mangrove stands. They are thought to play an important role in lignocellulosic degradation in such marine ecosystems (9). Commonly isolated marine fungi belong to ascomycetes and deuteromycetes, while basidiomycetes are relatively rarely reported (22,27,36). The lignocellulolytic enzymes of marine fungi have potential industrial and environmental applications (22,27,29).White rot fungi have a unique ability to decompose wood lignin via the secretion of extracellular lignin-degrading enzymes such as manganese peroxidase (MnP), lignin peroxidase, versatile peroxidase, and laccase. MnP is considered to be one of the key enzymes involved in lignin degradation caused by white rot fungi. MnP oxidizes Mn 2ϩ to Mn 3ϩ in an H 2 O 2 -dependent reaction, and Mn 3ϩ organic acid chelates oxidize monomeric phenol, phenolic lignin dimers, and synthetic lignin via the formation of a phenoxy radical (7,19). Additionally, MnP participates in lignin biodegradation via thiol and lipidderived free radicals that are able to oxidize a variety of nonphenolic aromatic compounds (1, 35). Although many genes encoding MnP have been cloned from several white rot fungi, there has been no report focusing on marine fungi.The marine fungus Phlebia sp. strain MG-60 was selected from 28 mushrooms and driftwoods collected from mangrove stands in Okinawa, Japan, based on PolyR-478 decolorization and lignin biodegradation under hypersaline conditions (15). Phlebia sp. strain MG-60 produces MnP mainly under hypersaline conditions. It was able to brighten the unbleached hardwood kraft pulp extensively even under conditions of 5% (wt/ vol) sea salts. In contrast, pulp was only slightly brightened by the widely studied white rot fungus Phanerochaete chrysosporium at 3% (wt/vol) and 5% (wt/vol) sea salt concentrations (15, 16). Thus, MG-60 has significant bleaching ability, especially in a hypersaline environment.To clarify the effect of hypersaline conditions on MnP production from Phlebia sp. strain MG-60, herein, we compare the productions of MnP activity and different MnP isozymes under normal and hypersaline conditions. We also provide the full sequences of three new MnP-encoding genes, MGmnp1, MGmnp2, and MGmnp3, which are differentially regulated in response to saline stress. MATERIALS AND METHODSFungal cultures. Phlebia sp. strain MG-60 TUFC40001 (Fungus/Mushroom Resource and Research Center, Tottori, Japan), Trametes versicolor NBRC6482, and Phanerochaete chrysosporium ATCC 34541 were maintained on potato dextrose agar (PDA) plates. Mycelium mats on an agar plate were transferred into a sterilized blender cup containing 5...

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“…Three MnP genes previously cloned from C. subvermispora (GenBank TM accession numbers AAB03480, AAD45725, and AAO61784 and described as MnP1, MnP3, and MnP4, respectively) (32,33) were identified (as models 116608, 139965, and 94398, respectively, showing 98 -100% identities with the corresponding GenBank TM sequences). C. subvermispora MnP2 (GenBank TM entries AAB92247 and AAD43581 with 99% sequence identity) was not localized in the genome but could be an allelic variant of gene 50297 (94% identity).…”

Section: Methodsmentioning

confidence: 99%

Lignin-degrading Peroxidases from Genome of Selective Ligninolytic Fungus Ceriporiopsis subvermispora

Fernández‐Fueyo

Ruíz-Dueñas

Miki

et al. 2012

Journal of Biological Chemistry

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Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Cited by 48 publications

References 30 publications

Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Saline-Dependent Regulation of Manganese Peroxidase Genes in the Hypersaline-Tolerant White Rot Fungus Phlebia sp. Strain MG-60

Lignin-degrading Peroxidases from Genome of Selective Ligninolytic Fungus Ceriporiopsis subvermispora

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