Characterization of the porcinetransferringene (TF ) and its association with disease severity following an experimentalActinobacillus pleuropneumoniaeinfection

Danilowicz, Emilia; Martínez‐Arias, Rosa; Dolf, G.; Singh, Manisha; Probst, Inga; Tümmler, Burkhard; Höltig, Doris; Waldmann, K.-H.; Gerlach, Gerald-F.; Stanke, Frauke; Leeb, Tosso

doi:10.1111/j.1365-2052.2009.02012.x

Cited by 7 publications

(9 citation statements)

References 19 publications

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“…The fact that more than 50 App genes are involved in iron uptake and metabolism (Xu et al., ), some of which are differentially expressed during infection (Deslandes et al., ; Klitgaard, Friis, Jensen, Angen, & Boye, ), supports the major importance of this bacterial adaptation strategy for disease pathogenesis (Bossé et al., ). Genetically defined differences in disease susceptibility between breeding lines were, in part, due to differences in the iron‐transport protein transferrin, because different protein variants might be bound with variable intensity by the transferrin binding receptors of App (Daniłowicz et al., ; Reiner, Bertsch, et al., ).…”

Section: Disease Pathogenesismentioning

confidence: 99%

“…Genetically defined differences in disease susceptibility between breeding lines were, in part, due to differences in the iron-transport protein transferrin, because different protein variants might be bound with variable intensity by the transferrin binding receptors of App (Daniłowicz et al, 2010;.…”

Section: Experimental Dual Infections With Swine Influenza Virus (Sivmentioning

confidence: 99%

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Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

144

178

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Summary Porcine pleuropneumonia, caused by the bacterial porcine respiratory tract pathogen Actinobacillus pleuropneumoniae, leads to high economic losses in affected swine herds in most countries of the world. Pigs affected by peracute and acute disease suffer from severe respiratory distress with high lethality. The agent was first described in 1957 and, since then, knowledge about the pathogen itself, and its interactions with the host, has increased continuously. This is, in part, due to the fact that experimental infections can be studied in the natural host. However, the fact that most commercial pigs are colonized by this pathogen has hampered the applicability of knowledge gained under experimental conditions. In addition, several factors are involved in development of disease, and these have often been studied individually. In a DISCONTOOLS initiative, members from science, industry and clinics exchanged their expertise and empirical observations and identified the major gaps in knowledge. This review sums up published results and expert opinions, within the fields of pathogenesis, epidemiology, transmission, immune response to infection, as well as the main means of prevention, detection and control. The gaps that still remain to be filled are highlighted, and present as well as future challenges in the control of this disease are addressed.

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Section: Disease Pathogenesismentioning

confidence: 99%

Section: Experimental Dual Infections With Swine Influenza Virus (Sivmentioning

confidence: 99%

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

144

178

View full text Add to dashboard Cite

show abstract

“…Recent evidence for genetic resistance/susceptibility of pigs to A. pleuropneumoniae, a serious factor in worldwide pig production, comes from direct line comparisons (Hoeltig et al 2009;Benga et al 2009;Danilowicz et al 2009;Kahlisch et al 2009) and two QTL studies (Gregersen et al 2010;Reiner et al 2014). Gregersen et al (2010) mapped QTL for chronic pleuritis in a vast number of slaughter pigs, however, with minor accuracy regarding field phenotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Recent data provided clear indications for a significant genetic difference in susceptibility to an A. pleuropneumoniae infection in challenged German breeding lines (Hoeltig et al 2009). The porcine transferrin gene (TF; Danilowicz et al 2009), glycoproteins (Kahlisch et al 2009), and immune markers (Benga et al 2009) have been regarded as potential corresponding biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Pathway deregulation and expression QTLs in response to Actinobacillus pleuropneumoniae infection in swine

et al. 2014

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Actinobacillus (A.) pleuropneumoniae is among the most important pathogens in pig. The agent causes severe economic losses due to decreased performance, the occurrence of acute or chronic pleuropneumonia, and an increase in death incidence. Since therapeutics cannot be used in a sustainable manner, and vaccination is not always available, new prophylactic measures are urgently needed. Recent research has provided evidence for a genetic predisposition in susceptibility to A. pleuropneumoniae in a Hampshire × German Landrace F2 family with 170 animals. The aim of the present study is to characterize the expression response in this family in order to unravel resistance and susceptibility mechanisms and to prioritize candidate genes for future fine mapping approaches. F2 pigs differed distinctly in clinical, pathological, and microbiological parameters after challenge with A. pleuropneumoniae. We monitored genome-wide gene expression from the 50 most and 50 least susceptible F2 pigs and identified 171 genes differentially expressed between these extreme phenotypes. We combined expression QTL analyses with network analyses and functional characterization using gene set enrichment analysis and identified a functional hotspot on SSC13, including 55 eQTL. The integration of the different results provides a resource for candidate prioritization for fine mapping strategies, such as TF, TFRC, RUNX1, TCN1, HP, CD14, among others.

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“…Daniłowicz et al [83] identified 62 polymorphisms in TF genome sequence on a panel of 10 different pig breeds, but only found one possible association of the severity of A. pleuropneumoniae infection with TF genotypes. Cav1 was differentially expressed under H. parasuis infection, SNPs in this gene were found associated with blood parameter traits [89].…”

Section: Genetic Control Of Porcine Resistance To Gram-negative Bacilmentioning

confidence: 99%

Immunogenomics for identification of disease resistance genes in pigs: a review focusing on Gram-negative bacilli

Song

Zhu

Chen

2012

J Animal Sci Biotechnol

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Over the past years, infectious disease has caused enormous economic loss in pig industry. Among the pathogens, gram negative bacteria not only cause inflammation, but also cause different diseases and make the pigs more susceptible to virus infection. Vaccination, medication and elimination of sick pigs are major strategies of controlling disease. Genetic methods, such as selection of disease resistance in the pig, have not been widely used. Recently, the completion of the porcine whole genome sequencing has provided powerful tools to identify the genome regions that harboring genes controlling disease or immunity. Immunogenomics, which combines DNA variations, transcriptome, immune response, and QTL mapping data to illustrate the interactions between pathogen and host immune system, will be an effective genomics tool for identification of disease resistance genes in pigs. These genes will be potential targets for disease resistance in breeding programs. This paper reviewed the progress of disease resistance study in the pig focusing on Gram-negative bacilli. Major porcine Gram-negative bacilli and diseases, suggested candidate genes/pathways against porcine Gram-negative bacilli, and distributions of QTLs for immune capacity on pig chromosomes were summarized. Some tools for immunogenomics research were described. We conclude that integration of sequencing, whole genome associations, functional genomics studies, and immune response information is necessary to illustrate molecular mechanisms and key genes in disease resistance.

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Characterization of the porcinetransferringene (TF ) and its association with disease severity following an experimentalActinobacillus pleuropneumoniaeinfection

Cited by 7 publications

References 19 publications

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Pathway deregulation and expression QTLs in response to Actinobacillus pleuropneumoniae infection in swine

Immunogenomics for identification of disease resistance genes in pigs: a review focusing on Gram-negative bacilli

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