Characterization of the photosynthetic apparatus of the Eustigmatophycean Nannochloropsis gaditana: Evidence of convergent evolution in the supramolecular organization of photosystem I

Basso, Stefania; Simionato, Diana; Gerotto, Caterina; Segalla, Anna; Giacometti, Giorgio M.; Morosinotto, Tomas

doi:10.1016/j.bbabio.2013.11.019

Cited by 51 publications

(43 citation statements)

References 71 publications

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“…Above this value both these parameters decrease (Table 5). With regard to N. gaditana pigments, several authors [2,34,37] have established that violaxanthin, vaucheriaxanthin and β-carotene are the most abundant carotenoids. Similarly, Takaichi [17] has reported that β-carotene, violaxanthin and vaucheriaxanthin are primary carotenoids in Eustigmatophyceae strains due to their importance for photosystem II.…”

Section: Influence Of Dilution Rate On High Value Productsmentioning

confidence: 99%

The influence of culture conditions on biomass and high value product generation by Nannochloropsis gaditana in aquaculture

et al. 2015

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Section: Influence Of Dilution Rate On High Value Productsmentioning

confidence: 99%

The influence of culture conditions on biomass and high value product generation by Nannochloropsis gaditana in aquaculture

et al. 2015

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“…However, some Nannochloropsis -specific features in the pigmentation and photosystem ratio have been recognized. Unlike many other heterokont algae, which utilize both chlorophyll a and c in their light harvesting complexes, Nannochloropsis only possesses chlorophyll a [19]. Also, contrary to green algae and plants, where the ratio between PSI and PSII has been shown to lie between 0.53–0.67 [20], the PSI to PSII ratio in Nannochloropsis is about 1.7 [21].…”

Section: Introductionmentioning

confidence: 99%

The plastoquinone pool of Nannochloropsis oceanica is not completely reduced during bright light pulses

2017

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The lipid-producing model alga Nannochloropsis oceanica has a distinct photosynthetic machinery. This organism possesses chlorophyll a as its only chlorophyll species, and has a high ratio of PSI to PSII. This high ratio of PSI to PSII may affect the redox state of the plastoquinone pool during exposure to light, and consequently may play a role in activating photoprotection mechanisms. We utilized pulse-amplitude modulated fluorometry to investigate the redox state of the plastoquinone pool during and after bright light pulses. Our data indicate that even very intense (5910 μmol photons s-1m-2 of blue light having a wavelength of 440 nm) light pulses of 0.8 second duration are not sufficient to completely reduce the plastoquinone pool in Nannochloropsis. In order to achieve extensive reduction of the plastoquinone pool by bright light pulses, anaerobic conditions or an inhibitor of the photosynthetic electron transport chain has to be utilized. The implication of this finding for the application of the widely used saturating pulse method in algae is discussed.

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“…PSI‐LHC can be purified from N. gaditana thylakoids in supercomplexes containing the core complex with its associated antenna (Basso et al ., ). The structure of PSI‐LHC from N. gaditana purified after solubilization with either α‐ or β‐DM (PSI‐LHC α and PSI‐LHC β ) was investigated using electron microscopy (EM).…”

Section: Resultsmentioning

confidence: 97%

“…f,g). β‐DM gives harsher solubilization than α‐DM (Basso et al ., ) and PSI‐LHC β samples showed the presence of monomers with < 1% of the particles that could be classified as dimers. An equivalent P700 : Chl ratio was measured in PSI‐LHC α and PSI‐LHC β supporting the hypothesis that monomers and dimers have similar composition (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

Conservation of core complex subunits shaped the structure and function of photosystem I in the secondary endosymbiont alga Nannochloropsis gaditana

et al. 2016

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Summary Photosystem I (PSI) is a pigment protein complex catalyzing the light‐driven electron transport from plastocyanin to ferredoxin in oxygenic photosynthetic organisms. Several PSI subunits are highly conserved in cyanobacteria, algae and plants, whereas others are distributed differentially in the various organisms.Here we characterized the structural and functional properties of PSI purified from the heterokont alga Nannochloropsis gaditana, showing that it is organized as a supercomplex including a core complex and an outer antenna, as in plants and other eukaryotic algae.Differently from all known organisms, the N. gaditana PSI supercomplex contains five peripheral antenna proteins, identified by proteome analysis as type‐R light‐harvesting complexes (LHCr4‐8). Two antenna subunits are bound in a conserved position, as in PSI in plants, whereas three additional antennae are associated with the core on the other side. This peculiar antenna association correlates with the presence of PsaF/J and the absence of PsaH, G and K in the N. gaditana genome and proteome.Excitation energy transfer in the supercomplex is highly efficient, leading to a very high trapping efficiency as observed in all other PSI eukaryotes, showing that although the supramolecular organization of PSI changed during evolution, fundamental functional properties such as trapping efficiency were maintained.

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Characterization of the photosynthetic apparatus of the Eustigmatophycean Nannochloropsis gaditana: Evidence of convergent evolution in the supramolecular organization of photosystem I

Cited by 51 publications

References 71 publications

The influence of culture conditions on biomass and high value product generation by Nannochloropsis gaditana in aquaculture

The influence of culture conditions on biomass and high value product generation by Nannochloropsis gaditana in aquaculture

The plastoquinone pool of Nannochloropsis oceanica is not completely reduced during bright light pulses

Conservation of core complex subunits shaped the structure and function of photosystem I in the secondary endosymbiont alga Nannochloropsis gaditana

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