Characterization of the lipid profile of Antarctic brown seaweeds and their endophytic fungi by gas chromatography–mass spectrometry (GC–MS)

Teixeira, Thaiz Rodrigues; Santos, Gustavo Souza; Turatti, Izabel Cristina Casanova; Paziani, Mário Henrique; Kress, Márcia Regina von Zeska; Colepicolo, Pio; Debonsi, Hosana Maria

doi:10.1007/s00300-019-02529-w

Cited by 22 publications

(16 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sterol composition presented fucosterol (14.5 %) as the major constituent of this class of compounds, followed by stigmasterol (7.9 %), desmosterol (1.0 %), and brassicasterol (0.3 %). Our investigation of the D. antarctica chemical profile corroborates with previous investigations that show palmitic acid as the most predominant FA in the Antarctic brown macroalgae Ascoseira mirabilis, Adenocystis utricularis, and Phaeurus antarcticus [20]. The composition of macroalgal FAs varies in quality and quantity according to different environmental conditions such as light, temperature, and salinity [21,22].…”

Section: Resultssupporting

confidence: 90%

GC-MS Analysis, Bioactivity-based Molecular Networking and Antiparasitic Potential of the Antarctic Alga Desmarestia antarctica

Santos

Rangel

Teixeira

et al. 2020

Planta Medica International Open

Self Cite

View full text Add to dashboard Cite

Leishmaniasis, malaria, and neosporosis are parasitic diseases that affect humans and animals, causing public health problems and billions in economic losses. Despite the advances in the development of new drugs, the severe side effects of available leishmaniasis treatments, the Plasmodium spp. resistance to antimalarial drugs, and the lack of a specific treatment against neosporosis lead us to the search for new anti-protozoan molecules from underexplored sources such as the Antarctic marine environment. Herein, we describe for the first time the chemical profile of Desmarestia antarctica crude extract and fractions using GC-MS and LC-MS/MS (molecular networking) approaches, and evaluate their antiparasitic activity against Leishmania amazonensis, Neospora caninum, and multi-drug-resistant Plasmodium falciparum. Furthermore, the cytotoxicity in 3T3 BALB/c fibroblasts and Vero cells was evaluated. D. antarctica fraction E ( IC50 of 53.8±4.4 μg mL− 1 and selectivity index of 3.3) exhibited anti-promastigote activity and was fourfold more selective to L. amazonensis rather than to the host cells. D. antarctica fraction D (IC50 of 1.6±1.3 μg mL− 1 and selectivity index of 27.8), D. antarctica fraction F (IC50 of 3.1±2.1 μg mL− 1 and selectivity index of 23.1), and D. antarctica fraction H (IC50 of 3.1±2.0 μg mL− 1 and selectivity index of 12.9) presented the highest antiparasitic effects against N. caninum with no cytotoxic effects. Also, D. antarctica fraction D presented a significant antiplasmodial inhibitory effect (IC50 of 19.1±3.9 μg mL− 1 and selectivity index of 6.0). GC-MS analysis indicated palmitic acid, myristic acid, fucosterol, phthalic acid, di(2-methylbutyl) ester, loliolide, and neophytadiene as the main components in the active fractions. In addition, this is the first report of a biological screening of macroalgae secondary metabolites against N. caninum parasites.

show abstract

Section: Resultssupporting

confidence: 90%

GC-MS Analysis, Bioactivity-based Molecular Networking and Antiparasitic Potential of the Antarctic Alga Desmarestia antarctica

Santos

Rangel

Teixeira

et al. 2020

Planta Medica International Open

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, PUFAs play key roles in electron and oxygen transport and tissue metabolism, including the regulation of membrane fluidity, as well as thermal adaptation (Funk 2001;Santos et al 2019). Additionally, public perception of healthy food and lifestyle has brought PUFAs to the attention of the consumers (Pereira et al 2017;Teixeira et al 2019). It is increasing the interest in a typical PUFAs family ( ω-3) named eicosapentaenoic acid 8,11,14,17 ω-3).…”

Section: Macroalgaementioning

confidence: 99%

Bioprospecting macroalgae, marine and terrestrial invertebrates & their associated microbiota

et al. 2022

Self Cite

View full text Add to dashboard Cite

The present review aims the discussion of the impact of the bioprospection initiative developed by the projects associated to BIOprospecTA, a subprogram of the program BIOTA, supported by FAPESP. This review brings a summary of the main results produced by the projects investigating natural products (NPs) from non-plants organisms, as examples of the success of this initiative, focusing on the progresses achieved by the projects related to NPs from macroalgae, marine invertebrates, arthropods and associated microorganisms. Macroalgae are one of the most studied groups in Brazil with the isolation of many bioactive compounds including lipids, carotenoids, phycocolloids, lectins, mycosporine-like amino acids and halogenated compounds. Marine invertebrates and associated microorganisms have been more systematically studied in the last thirty years, revealing unique compounds, with potent biological activities. The venoms of Hymenopteran insects were also extensively studied, resulting in the identification of hundreds of peptides, which were used to create a chemical library that contributed for the identification of leader models for the development of antifungal, antiparasitic, and anticancer compounds. The built knowledge of Hymenopteran venoms permitted the development of an equine hyperimmune serum anti honeybee venom. Amongst the microorganisms associated with insects the bioprospecting strategy was to understand the molecular basis of intra- and interspecies interactions (Chemical Ecology), translating this knowledge to possible biotechnological applications. The results discussed here reinforce the importance of BIOprospecTA program on the development of research with highly innovative potential in Brazil.

show abstract

“…The third distilled water of the washing procedure is also inoculated in agar plates, aiming to check no microorganism's growth (negative control 2). To the selective isolation of fungi, the culture medium utilized is supplemented with chloramphenicol (200 mg•L −1 ) [38].…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

Antitumor Potential of Seaweed Derived-Endophytic Fungi

et al. 2019

Self Cite

View full text Add to dashboard Cite

The marine environment presents a high biodiversity and a valuable source of bioactive compounds with therapeutic and biotechnological potential. Among the organisms present in marine environment, the endophytic fungi isolated from seaweed stand out. These microorganisms have aroused interest in the scientific community regarding its various activities such as antiviral, antimicrobial, antioxidant, photoprotective, cytotoxic, genotoxic, anti-inflammatory, and anticancer, besides establishing important ecological relations with its hosts. Anticancer molecules derived from marine natural sources are a promising target against different types of cancer. The disease’s high rates of morbidity and mortality affect millions of people world wild and the search for new therapeutic alternatives is needed. Thus, this review partially summarizes the methodologies for the isolation of seaweed-derived endophytic fungi, as well as describes the anticancer compounds isolated from such microorganisms, reported in the literature from 2009 to the present. In addition, it describes how some biotechnological processes can help in the discovery of bioactive compounds, especially with anticancer activity.

show abstract

Characterization of the lipid profile of Antarctic brown seaweeds and their endophytic fungi by gas chromatography–mass spectrometry (GC–MS)

Cited by 22 publications

References 90 publications

GC-MS Analysis, Bioactivity-based Molecular Networking and Antiparasitic Potential of the Antarctic Alga Desmarestia antarctica

GC-MS Analysis, Bioactivity-based Molecular Networking and Antiparasitic Potential of the Antarctic Alga Desmarestia antarctica

Bioprospecting macroalgae, marine and terrestrial invertebrates & their associated microbiota

Antitumor Potential of Seaweed Derived-Endophytic Fungi

Contact Info

Product

Resources

About