Characterization of the immunoprotective antigen of ribosomal preparations from Haemophilus influenzae

Tewari, Ram P.; Lynn, M; Birnbaum, A J; Solotorovsky, Morris

doi:10.1128/iai.19.1.58-65.1978

Cited by 21 publications

(16 citation statements)

References 30 publications

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“…The ribosomal suspension was standardized on the basis of protein content by the method of Lowry et al (8). The chemical composition of the ribosomes was similar to that previously described (10,13). The relative amounts of ribonucleic acid, protein, and hexose were 65, 35, and 0%, respectively.…”

Section: Methodssupporting

confidence: 68%

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Serological and biological activities of anti-Haemophilus influenzae ribosomal serum

Katz¹,

Lynn²,

Solotorovsky³

1981

Infect Immun

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The antibody content in serum from rabbits immunized with ribosomes from Haemophilus influenzae type b was determined by passive hemagglutination, enzyme-linked immunosorbent assay, and complement fixation. Attempts to use passive hemagglutination to assay anti-ribosomal antibodies were unsuccessful. In the enzyme-linked immunosorbent assay tests, rabbit antiserum was allowed to react with ribosomes that adhered to microtiter plates. The enzyme-linked immunosorbent assay method detected, in two ribosome-immunized rabbits and by 3 days postimmunization, titers which rose to plateaus on days 24 to 31 and declined thereafter. With the complement fixation method, the serum from one immunized rabbit also showed a titer on day 3 and reached a plateau on days 20 to 31. Serum from the other immunized rabbit did not develop a titer until day 11; it reached a lower plateau on days 20 to 24 and then declined on days 27 to 55. Although there were no apparent differences between the two immunized rabbits by the enzyme-linked immunosorbent assay, there were differences between the complement fixation antibodies in these rabbits. Passive protection experiments were performed with these sera. Maximal passive protection was achieved when mice were challenged intraperitoneally with 100 50% lethal doses of H. influenzae and immunized intravenously 1 h later with rabbit serum collected 27 days postimmunization. Rabbit anti-ribosomal sera were evaluated for bactericidal activity. Undiluted immune sera showed bactericidal activity; however, when diluted 1:10, activity was lost. Although bactericidal activities of immune sera were correlated to passive protection activities, it is unlikely that such protection was due to bactericidal antibodies. Immune serum had opsonizing activity since it enhanced phagocytosis of H. influenzae by mouse leukocytes.

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Section: Methodssupporting

confidence: 68%

“…It has been reported that ribosomes from Haemophilus influenzae induce active immunity against a lethal H. influenzae type b infection in experimental animals; ribosomes were free from detectable capsule and cell wall components, and protein was the immunogenic moiety in these ribosomes (10,13).…”

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Serological and biological activities of anti-Haemophilus influenzae ribosomal serum

Katz¹,

Lynn²,

Solotorovsky³

1981

Infect Immun

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“…Although thevalueofribosomal vaccines is now established, doubt remains as to their mechanism ofaction and as to the exact nature of the immunogenic principle responsible for their activity. Many authors have demonstrated the presence of small amounts of surface antigens in ribosomal preparations from Streptococcus pneumoniae (3,4), Pasteurella multocida ( 5 ) , Vibrio cholerae (1 7), Salmonella typhimurium (7,18,23) and Hemophilus influenzae (32). In a previous study, we have shown that polysaccharide antigens were also strongly bound to the r-RNA isolated from various strains (24).…”

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confidence: 92%

Role of the Polysaccharide and of the Lipopolysaccharide in the Immunoprotective Capacity of Klebsiella Pneumoniae Ribosomes

Normier

Lafont²,

D'Hinterland

et al. 2009

Acta Pathologica Microbiologica Scandinavica Series C: Immunology

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Ribosomes, ribosomal RNA (r‐RNA), capsular polysaccharide (PS‐K) and lipopolysaccharide (LPS) were isolated and purified from Klebsiella pneumoniae, type I. The protective capacity of these different fractions was investigated in function of their analytical composition. The results show that ribosomes, and in particular, ribosomal RNA have the greatest protective activity at the lowest concentrations. The role of PS‐K in the specific immune response to ribosomes was also investigated. Even at very high levels PS‐K failed to afford any significant protection. LPS gave no protection. A stable r‐RNA/PS‐K complex was isolated by means of affinity chromatography. This complex was uncontaminated by LPS and its existence indicated strong bonding between surface polysaccharide antigens and r‐RNA. The r‐RNA/PS‐K complex was not antigenic in itself but conferred good protection when combined with an adjuvant.

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“…Bacteria used in bactericidal and opsonophagocytic studies and for adsorption were grown in Mueller-Hinton broth supplemented with 10 ,ug each of hemin and NAD (Sigma Chemical Co., St. Louis, Mo.) per ml at 37°C; incubation was for about 18 30 60 90 120 TIME (MINUTES) FIG. 1.…”

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confidence: 99%

“…(2,3,10,17,19,28,30). It is reasonable to believe that these same kinds of antibody may contribute to immunity to NT H. influenzae.INFECT.…”

mentioning

confidence: 99%

Opsonizing and bactericidal effects of normal human serum on nontypable Haemophilus influenzae

Musher¹,

Hague-Park²,

Baughn³

et al. 1983

Infect Immun

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The observation that nontypable (NT) Haemophilus influenzae causes serious infection in adults has stimulated interest in mechanisms that may protect the human host against NT H. influenzae infection. Incubating NT H. influenzae with normal human serum (NHS) caused doseand time-dependent killing that varied with the individual NHS and NT H. influenzae. Adsorption of NHS with NT H. influenzae removed bactericidal activity against the adsorbing isolate but not necessarily that against others, suggesting antigenic diversity and supporting recent studies that show different outer membrane protein profiles among NT H. influenzae. Heating NHS to 56°C for 30 min abolished bactericidal activity; this activity was not restored by complement-rich guinea pig serum or NT H. influenzae-adsorbed NHS. This is analogous to the "third factor" needed for intraleukocytic killing of pneumococci. Optimal opsonization of NT H. influenzae for phagocytosis by human polymorphonuclear leukocytes required antibody and complement, but other serum factors also played a role. Bactericidal activity generally, but not uniformly, correlated with opsonizing activity of individual NHS. Humoral factors may be important in host defenses against NT H. influenzae infection; their emergence study. during convalescence warrants further Although Haemophilus influenzae type b remains the predominant pathogenic H. influenzae for infants and children (27) and is far more virulent than other typable or nontypable H. influenzae in animal models (18, 21), recent clinical evidence has implicated nontypable (NT) H. influenzae as a surprisingly important pathogen in adults (5, 26, 31; R. Rev. Infect. Dis., in 297 on July 6, 2020 by guest http://iai.asm.org/ Downloaded from INFECT. IMMUN.

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Characterization of the immunoprotective antigen of ribosomal preparations from Haemophilus influenzae

Cited by 21 publications

References 30 publications

Serological and biological activities of anti-Haemophilus influenzae ribosomal serum

Serological and biological activities of anti-Haemophilus influenzae ribosomal serum

Role of the Polysaccharide and of the Lipopolysaccharide in the Immunoprotective Capacity of Klebsiella Pneumoniae Ribosomes

Opsonizing and bactericidal effects of normal human serum on nontypable Haemophilus influenzae

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