2015
DOI: 10.1128/mcb.00601-15
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Abstract: bRestores TBP function 1 (Rtf1) is generally considered to be a subunit of the Paf1 complex (PAF1C), a multifunctional protein complex involved in histone modification and transcriptional or posttranscriptional regulation. Rtf1, however, is not stably associated with the PAF1C in most species except Saccharomyces cerevisiae, and its biochemical functions are not well understood. Here, we show that human Rtf1 is a transcription elongation factor that may function independently of the PAF1C. Rtf1 requires "Rtf1 … Show more

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Cited by 39 publications
(34 citation statements)
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References 57 publications
(107 reference statements)
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“…6a), showing that Paf1C is globally required for normal Pol II transcription. Furthermore, the analysis of significantly downregulated transcripts in paf1Δ and rtf1Δ strains with a Venn diagram shows strong overlap between paf1Δ and rtf1Δ strains, but that the rtf1Δ strain shows 35% more downregulated RNAs, consistent with the idea that Rtf1 has Paf1C-independent roles34 (Fig. 6b).…”
Section: Resultssupporting
confidence: 76%
“…6a), showing that Paf1C is globally required for normal Pol II transcription. Furthermore, the analysis of significantly downregulated transcripts in paf1Δ and rtf1Δ strains with a Venn diagram shows strong overlap between paf1Δ and rtf1Δ strains, but that the rtf1Δ strain shows 35% more downregulated RNAs, consistent with the idea that Rtf1 has Paf1C-independent roles34 (Fig. 6b).…”
Section: Resultssupporting
confidence: 76%
“…Some organisms, including humans, contain an additional subunit, the multifunctional Ski8/Wdr61 protein [29]. Moreover, in organisms other than budding yeast, ranging from fission yeast to humans, Rtf1 is not strongly associated with Paf1C and has been shown to function independently of other Paf1C subunits in certain contexts [18, 30, 31]. Although none of the subunits is essential in budding yeast, cells lacking Paf1 or Ctr9 have severe growth defects [32], and Paf1C orthologs are essential in higher organisms [33, 34].…”
Section: Mechanisms Of Targeting Paf1c To Chromatinmentioning
confidence: 99%
“…Both Spt5 and the Pol II CTD are substrates of the same kinase, Bur1 (CDK9 in humans), and the activity of this kinase is required for proper Paf1C recruitment in both yeast and human cells [16, 30, 41, 42, 45]. However, consistent with the weak association between Rtf1 and other Paf1C subunits in metazoans, a structure/function analysis of human Rtf1 suggests that Paf1C recruitment in humans may be primarily Rtf1-independent, at least at certain genes [31]. Interestingly, a recent cryo-EM analysis revealed that yeast Paf1C has a tripartite, mobile structure that contacts an extended region on the Pol II outer surface in the context of an elongation complex [46].…”
Section: Mechanisms Of Targeting Paf1c To Chromatinmentioning
confidence: 99%
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“…Prominent among these is the multi-functional Paf1C. Paf1C promotes H2Bub in vivo (Ng et al, 2003a; Wood et al, 2003b; Xiao et al, 2005), and this function is dependent on a small, conserved domain within the Rtf1 subunit, both in yeast (Piro et al, 2012; Warner et al, 2007) and in higher eukaryotes (Cao et al, 2015). This domain, termed the Rtf1 histone modification domain (hereafter, HMD), is required for H2B K123ub in S. cerevisiae (Tomson et al, 2011; Warner et al, 2007), and expression of the HMD is sufficient to restore global H2B K123ub in a yeast strain deleted of the endogenous RTF1 gene (Piro et al, 2012).…”
Section: Introductionmentioning
confidence: 99%