2002
DOI: 10.1081/rrs-120014589 View full text |Buy / Rent full text
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Abstract: The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors. Eight human AC isoforms, encoded by separate genes, have been identified up to now. However, in several cases only partial cDNA sequences are available (ADCY1,2,5). A ninth expected isoform, the human ortholog of rat ADCY4, has not been described yet. Using the high inter-species homology of mammalian AC isoforms, we searched the human genome and we succeeded to identify full-leng… Show more

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“…15 Western blots using the selective antibody for the AC-VI isoform ( Figure 1B) did not show a band at 130 kDa 16 where it would be expected. A very faint band was barely apparent in this lane just below the 130-kDa site.…”
Section: Ac-v and Ac-vi Isoform Expression In Jg Cells Using Western mentioning
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“…15 Western blots using the selective antibody for the AC-VI isoform ( Figure 1B) did not show a band at 130 kDa 16 where it would be expected. A very faint band was barely apparent in this lane just below the 130-kDa site.…”
Section: Ac-v and Ac-vi Isoform Expression In Jg Cells Using Western mentioning
“…Proteins were electrophoretically transferred to a polyvinylidene difluoride membrane (polyvinylidene fluoride, Invitrogen) overnight at 4°C. The membranes were blocked with 5% milk in tris-buffered saline tween for 1 hour at room temperature and then incubated with either an antibody selective for the AC-V isoform (Fabgennix International Inc) 15 or an antibody selective for the AC-VI isoform (Abcam) 16 in 1:1000 dilutions in 5% albumin overnight at 4°C. Primary antibodies were labeled with a horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:2000.…”
Section: Ac-v and Ac-vi Isoform Expression In Jg Cells Using Western mentioning
“…There are nine types of adenylyl cyclases, which are all transmembrane proteins, synthesizing cAMP at the plasma membrane (73,74). This results in a gradient in which higher concentrations of cAMP are found at the membrane and lower concentrations in the cytosol (73,(75)(76)(77)(78).…”
Section: Role Of Alveolar Epithelial B 2 -Adrenergic Receptorsmentioning
“…The C1 and C2 regions are further subdivided in to "a" and "b" regions. The C1a and C2a regions interact to form the catalytic domain of adenylyl cyclase (Tang and Hurley, 1998;Ludwig and Seuwen, 2002). The transmembrane spanning domains have been suggested to be important in the regulation of protein assembly and membrane trafficking of the enzyme (Gu et al, 2001).…”
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