Characterization of the Genome of Insect Iridescent Virus Type 6 by Physical Mapping

Identification of the Gene Encoding the Major Capsid Protein of Insect Iridescent Virus Type 6 by Polymerase Chain Reaction

“…Hybridization was performed with a modified Southern blot technique (Southern, 1975) as described previously (Schnitzler et al, 1987). The nick translation of viral DNA or plasmid DNA was carried out using [~-z~P]dATP and [c~-32P]dCTP (New England Nuclear; specific activity 6000 Ci/mmol) according to Rigby et al (1977).…”

“…The recombinant plasmids harbouring all EcoRI DNA fragments of the CIV genome used in this study were obtained from a defined gene library representing 100% of the DNA sequence of the viral genome which was established as described elsewhere (Schnitzler et al, 1987;Soltau et al, 1987).…”

“…The CIV genome (209 kbp) is circularly permuted and terminally redundant (Delius et al, 1984;Schnitzler et al, 1987;Soltau et al, 1987;Fischer et al, 1990). CIV possesses a major capsid protein of about 50K (p50) as reported by Cerutti & Devauchelle (1985).…”

Insect iridescent virus type 6 encodes a polypeptide related to the largest subunit of eukaryotic RNA polymerase II

“…The recombinant plasmids harbouring all EcoRI DNA fragments of the CIV genome used in this study were obtained from a defined gene library representing 100 % of the sequence of the viral genome which was established as described elsewhere Soltau et aL, 1987).…”

“…The genome of this virus (209 kbp) is circularly permuted and terminally redundant (Delius et al, 1984;Schnitzler et al, 1987;Soltau et al, 1987;Fischer et al, 1990), a property in common with frog virus 3, fish lymphocystis disease virus (Darai et al, 1983) and iridescent virus type 9 (Ward & Kalmakoff, 1987). The genome of CIV has been characterized Soltau et al, 1987;Fischer et al, 1988a, b) and the positions of the origins of DNA replication have been determined (Handermann et al, 1992). One of at least six origins of replication is located in EcoRI fragment M of the CIV genome (7"1 kbp, 0.310 to 0.345 map units, m.u.…”