Characterization of the differential protein expression associated with thermoresistance in human gastric carcinoma cell lines

Abstract

Resistance to chemotherapeutic agents is one of the major problems faced during palliative therapy of tumor cells. Thus, chemotherapy is frequently combined with other modes of therapy such as radiation therapy and/or hyperthermia. Tumor cells respond to heat stress with development of thermotolerance and the interactions between chemo- and thermoresistance phenomena are not clearly understood. In this paper, we analyze the differential protein expression in vitro in human stomach cancer cells, their chemoresistant and thermoresistant counterparts using proteomics. The immediate aim was to identify sets of proteins that may lead to the development of thermoresistance. Based on these results, we aim to develop functional tests and methods for the modulation of thermoresistance and chemoresistance phenomena that may assist the therapy of inoperable cancers.

Two-dimensional differential in-gel electrophoresis for identification of gastric cancer-specific protein markers

“…HSPs are molecular chaperones which regulate the activity of multiple intracellular signaling intermediates, many of which are intimately involved in the control of the apoptotic signaling pathways. HSPs include anti-apoptotic and pro-apoptotic proteins that interact with a variety of cellular proteins (18). HSP27 and HSP70 are anti-apoptotic, while HSP60 and HSP10 are pro-apoptotic.…”

The Proteomics Approach to Find Biomarkers in Gastric Cancer

“…We obtained seven over-expressed proteins (NSP3, transgelin, prohibitin, HSP27, protein disulfide isomerase A3, unnamed protein product and glucose regulated protein) in cancer. In thermoresistant human gastric cancer cell line study, stratifin, cytokeratin 7-9, calreticulin, glucose regulated protein 78, and heat shock proteins (hsp27, hsx70 variant, hsp 27 variant) were reported as over-expressed protein (15). They declared that the development of thermoresistance in stomach cancer cell and their chemoresistance variants is a complex phenomenon that is not only associated with the expression of HSPs but of molecular chaperones at every subcellular level.…”

“…The extracellular proteins (serum albumin, serotransferrin, and alpha-1-antitrypsin) were identified as under-expressed proteins in cancer tissue. In thermoresistant cancer cell line study, eight proteins (14-3-3, aldehyde dehydrogenase 1, annexin 1, lipocortin 1 variant, initiation factor-5A, initiation factor-5A variant, reticulocalbin, and vimentin) were reported as under-expressed proteins (15).…”

Finite volume analysis of temperature effects induced by active MRI implants with cylindrical symmetry: 1. Properly working devices

“…Figures 7,8,9 show the results of these simulations. In no simulation the temperature increases reach a value of 5 K. Literature [17-21] on hyperthermia permits an estimation of the temperature resistance of normal animal cells and human cancer cells, depending on exposure time and temperature increase. The value chosen as physiologically critical, 5 K for 10 min to 15 min exposure time, is a worst case assumption, as various cells tolerate higher temperature increases as well as longer exposure times.…”