Characterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium by
            <i>Leuconostoc mesenteroides</i>
            NRRL B-1299

Dols, M. J. L.; Remaud‐Siméon, Magali; Willemot, René‐Marc; Vignon, M.R.; Monsan, Pierre

doi:10.1128/aem.64.4.1298-1302.1998

Cited by 64 publications

(22 citation statements)

References 34 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dextran hydrolysis by endodextranase requires a minimum of six or seven non-substituted contiguously linked ␣-(1,6) glucose residues (Boune, Huston, & Weigel, 1962;Côté & Robyt, 1984;Richards & Streamer, 1978). Thus, the decision was made to make certain that L. mesenteroides FT045B was producing a real dextran with predominantly ␣-(1,6)-glucopyranosidic linkages within the main chains prior to carrying out the other characterization methods (Buchholz & Monsan, 2001;Dols et al, 1998;Seymour & Knapp, 1980). The mixture of oligosaccharides obtained from FT045B dextran hydrolysis were applied to TLC (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Dextran is an extracellular bacterial homopolysaccharide (Sidebotham, 1974;Monsan et al, 2001) , the main chain of which is composed of contiguous ␣-(1,6)-linked d-glucopyranose residues and branches, stemming mainly side-chains of ␣-(1,6) glucose units attached by ␣-(1,3) branch linkages to ␣-(1,6)linked chains (Buchholz & Monsan, 2001;Dols, Remaud-Simeon, Willemot, Vignon, & Monsan, 1998;Naessens, Cerdobbel, Soetaert, & Vandamme, 2005;Robyt, 1995;Seymour & Knapp, 1980). Some dextrans have ␣-(1,2) or ␣-(1,4) branch linkages, depending on the bacterial source.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Vettori¹,

Franchetti²,

Contiero³

2012

Carbohydrate Polymers

107

View full text Add to dashboard Cite

This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1 H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% ␣-(1,6) linkages in the main chains and 2.1% ␣-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% ␣-(1,6) linkages in the main chains and 5% ␣-(1,3) branch linkages.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Vettori¹,

Franchetti²,

Contiero³

2012

Carbohydrate Polymers

107

View full text Add to dashboard Cite

show abstract

“…Diverse studies reported about the sucrose-inducible or pH-dependent release of dextransucrases in LAB [22,[33][34][35][36]. We recently observed that the dextransucrase of L. hordei is released into buffer supernatants efficiently in the presence of sucrose [24].…”

Section: Discussionmentioning

confidence: 99%

A systematic approach to study the pH-dependent release, productivity and product specificity of dextransucrases

et al. 2019

View full text Add to dashboard Cite

Background Dextransucrases are extracellular enzymes, which catalyze the formation of α-1→6-linked glucose polymers from sucrose. These enzymes are exclusively expressed by lactic acid bacteria, which commonly acidify the extracellular environment due to their physiology. Dextransucrases are thus confronted with steadily changing reaction conditions in regards to the environmental pH, which can further affect the amount of released dextransucrases. In this work, we studied the effect of the environmental pH on the release, the productivity and the product specificity of the dextransucrase expressed by Lactobacillus (L.) hordei TMW 1.1822. Dextransucrases were recovered as crude extracts at pH 3.5–pH 6.5 and then again used to produce dextrans at these pH values. The respectively produced dextran amounts and sizes were determined and the obtained results finally systematically correlated. Results Maximum dextran amounts were produced at pH 4.0 and pH 4.5, while the productivity of the dextransucrases significantly decreased at pH 3.5 and pH 6.5. The distribution of dextran amounts produced at different pH most likely reflects the pH dependent activity of the dextransucrases released by L. hordei, since different transglycosylation rates were determined at different pH using the same dextransucrase amounts. Moreover, similar hydrolysis activities were detected at all tested conditions despite significant losses of transglycosylation activities indicating initial hydrolysis prior to transglycosylation reactions. The molar masses and rms radii of dextrans increased up to pH 5.5 independently of the stability of the enzyme. The gelling properties of dextrans produced at pH 4.0 and pH 5.5 were different. Conclusions The presented methodological approach allows the controlled production of dextrans with varying properties and could be transferred and adapted to other microbes for systematic studies on the release and functionality of native sucrases or other extracellular enzymes.

show abstract

“…Since the 1950s, L. citreum NRRL B-1299 has received particular attention due to its ability to synthesize an unusual dextran containing up to 35% a-(1?2) linkages [26,[46][47][48][49]. Here we provide the assembled genome for this strain.…”

Section: Discussionmentioning

confidence: 99%

Inventory of the GH70 enzymes encoded by Leuconostoc citreum NRRL B‐1299 – identification of three novel α‐transglucosylases

et al. 2015

View full text Add to dashboard Cite

Leuconostoc citreum NRRL B‐1299 has long been known to produce α‐glucans containing both α‐(1→6) and α‐(1→2) linkages, which are synthesized by α‐transglucosylases of the GH70 family. We sequenced the genome of Leuconostoc citreum NRRL B‐1299 to identify the full inventory of GH70 enzymes in this strain. Three new genes (brsA, dsrM and dsrDP) putatively encoding GH70 enzymes were identified. The corresponding recombinant enzymes were characterized. Branching sucrase A (BRS‐A) grafts linear α‐(1→6) dextran with α‐(1→2)‐linked glucosyl units, and is probably involved in the α‐(1→2) branching of L. citreum NRRL B‐1299 dextran. This is the first report of a naturally occurring α‐(1→2) branching sucrase. DSR‐M and DSR‐DP are dextransucrases that are specific for α‐(1→6) linkage synthesis and mainly produce oligomers or short dextrans with molar masses between 580 and 27 000 g·mol−1. In addition, DSR‐DP contains sequences that diverge from the consensus sequences that are typically present in enzymes that synthesize linear dextran. Comparison of the genome with five other L. citreum genomes further revealed that dsrDP is unique to L. citreum NRRL B‐1299. The presence of this gene in a prophage represents the first evidence of phage‐mediated horizontal transfer of genes encoding such enzymes in lactic acid bacteria. Finally, brsA and dsrM are located in a chromosomal region in which genes encoding strain‐specific GH70 enzymes are consistently located. This region may be a good target on which to focus in order to rapidly evaluate the diversity of GH70 enzymes in L. citreum strains.

show abstract

Characterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium by Leuconostoc mesenteroides NRRL B-1299

Cited by 64 publications

References 34 publications

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

A systematic approach to study the pH-dependent release, productivity and product specificity of dextransucrases

Inventory of the GH70 enzymes encoded by Leuconostoc citreum NRRL B‐1299 – identification of three novel α‐transglucosylases

Contact Info

Product

Resources

About