Characterization of the complete mitochondrial genomes of Coronocyclus labiatus and Cylicodontophorus bicoronatus: Comparison with Strongylidae species and phylogenetic implication

Gao, Yuan; Wang, Xiaoxu; Ma, Xiao-Xiao; Zhang, Zhong-Huai; Zhuo, Lan; Qiu, Yang-Yuan; Wang, Shuang; Song, Min-Xin; Wang, Chun-Ren

doi:10.1016/j.vetpar.2021.109359

Cited by 5 publications

(8 citation statements)

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“…Mitochondrial genomes (mitogenomes) have maternal inheritance, an apparent lack of recombination, rapid evolutionary rates and comparatively conserved genomic structures ( Pyziel et al, 2020 ). Recently, sequences of whole mitogenomes have been used to infer phylogenetic relationships among parasites at different taxonomic levels ( Gao et al, 2021 ). For example, mitogenome dataset-based phylogenetic analyses showed that Parascaris equorum and Parascaris univalens are very closely related compared with other ascarids, and these mitogenome datasets provide genetic evidence that P. equorum and P. univalens represent the same species ( Gao et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the mitochondrial genome of Tetrameres grusi and insights into the phylogeny of Spirurina

Gao

Mao

et al. 2022

International Journal for Parasitology: Parasites and Wildlife

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Tetrameres grusi is a significant parasitic nematode of cranes that is classified into suborder Spirurina. However, for more than a century, this classification has been controversial. Mitochondrial genomes are valuable resources for parasite taxonomy, population genetics and systematics studies. Here, the mitochondrial genome of T. grusi was determined and subsequently compared with those from Spirurina species using concatenated datasets of amino acid sequences predicted from mitochondrial protein-coding genes. The complete mitochondrial genome of T. grusi is circular with 13,709 bp, and it contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one non-coding region. All of the protein-coding genes are transcribed in the same direction. There were 18 intergenic spacers of 1–44 bp, and six locations with gene overlaps, ranging from 1 bp to 28 bp, in the mitochondrial genome of T. grusi . The AT content of this mitochondrial genome was 71.56%. This was similar to mitochondrial genomes of other Spirurina species, which also exhibited strong AT content bias, not only in the nucleotide composition but also in codon usage. The sequenced mitogenomes of the 25 Spirurina nematodes showed three classes of gene arrangements based on the 12 protein-coding genes, and the gene arrangement of the T. grusi mitochondrial genome belonged to the Class I. Phylogenetic analyses using mitochondrial genomes of 25 Spirurina nematodes revealed that T. grusi (Habronematoidea) was closer to Gongylonema pulchrum (Spiruroidea) than Spirocerca lupi (Thelazioidea). The availability of the complete mitochondrial genome sequence of T. grusi provides new and useful genetic markers for further studies on Spirurina nematodes.

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Section: Introductionmentioning

confidence: 99%

Characterization of the mitochondrial genome of Tetrameres grusi and insights into the phylogeny of Spirurina

Gao

Mao

et al. 2022

International Journal for Parasitology: Parasites and Wildlife

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“…In previous studies, mt genomes have been used to address a lot of controversies on the status of certain taxa, such as the taxonomy of Triodontophorus , the identification of species complex, etc. [ 11 , 12 , 13 ]. Now, 13 complete mt genomes of Cyathostominae nematodes have been determined, including 5 complete mt genomes in Cylicocyclus [ 10 , 11 , 12 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

The Mitochondrial Genome of Cylicocyclus elongatus (Strongylida: Strongylidae) and Its Comparative Analysis with Other Cylicocyclus Species

Gao

Zhang

Wang

et al. 2022

Animals

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Cylicocyclus elongatus (Cc. elongatus) is one of the species in Cylicocyclus, subfamily Cyathostominae, but its taxonomic status in Cylicocyclus is controversial. Mitochondrial (mt) genome is an excellent gene marker which could be used to address the taxonomy controversy. In the present study, the complete mt genome of Cc. elongatus was determined, and sequence and phylogenetic analyses were performed based on mtDNA data to determine the classification of Cc. elongatus. The circular complete mt genome of Cc. elongatus was 13875 bp in size, containing 12 protein-coding genes (12 PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions (NCRs). The A + T content of Cc. elongatus complete mt genome was 76.64%. There were 19 intergenic spacers with lengths of 2–53 bp and 2 overlaps with lengths of 1–2 bp in the impact complete mt genome. ATT and TAA were the most common start and termination codons of 12 PCGs, respectively. Comparative analyses of mt genomes nucleotide sequence and amino acid sequence showed that there were higher identities between Cc. elongatus and five other Cylicocyclus, rather than with P. imparidentatum. Phylogenetic analyses based on concatenated nucleotide sequences of 12 PCGs of 23 species in the family Strongylidae showed that Cc. elongatus was closely related to Cylicocyclus species, rather than P. imparidentatum. We concluded that Cc. elongatus was a member in Cylicocyclus based on comparative and phylogenetic analyses of mt genome sequences. The data of the complete mt genome sequence of Cc. elongatus provide a new and useful genetic marker for further research on Cyathostominae nematodes.

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“… 2020 ; Gao et al. 2021 ), demonstrating the phylogenetic stability of these bursate nematodes found in equines. Taken together, the sequenced mitochondrial genome of C. auriculatus not only provides novel molecular evidence for its phylogenetic position in Cylicocyclus but also enriches the marker resource for molecular diagnostic, systematic and evolutionary biological studies of strongyloid nematodes.…”

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confidence: 97%

“… 2008 ; Zhang and Kong 2002 ; Gao et al. 2021 ). Under this context, we decoded the mitochondrial genome of C. auriculatus , a representative member of this genus from the Dezhou donkey in Southwest China using the Illumina sequencing technology, as the mitochondrial DNA is not only a rich resource for molecular markers but its complete data can also provide novel insights into phylogenetic relationships of Cylicocyclus (Jex et al.…”

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confidence: 99%

“…Building on a concatenated amino acid dataset of 12 protein-coding genes from 17 strongyloid parasites, the maximum parsimony (MP), maximum-likelihood (ML) and Bayesian inference (BI) methods were used to construct phylogenetic trees using Necator americanus Stiles 1902 as the outgroup because of its relationship with equine Strongyloidea nematodes (Jex et al., 2009 ; Liu et al., 2013 ; Gao et al., 2021 ). The three identical trees clearly grouped C. auriculatus with Cylicocyclus ashworthi Le Roux 1924, Cylicocyclus insigne Boulenger 1917, Cylicocyclus nassatus Looss 1900 and Cylicocyclus radiates Krecek 2011 and together formed a monophyletic relationship with either species of Cyathostomum , Coronocyclus , Cyathostomum , Cylicostephanus or Cylicodontophorus within Cyathostominae ( Figure 1 ).…”

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Complete mitochondrial genome of cylicocyclus auriculatus: molecular structure and phylogenetic analysis

Wang

Chen

et al. 2022

Mitochondrial DNA Part B

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Cylicocyclus spp. (Nematoda: Strongylida: Cyathostominae) are the common and important parasitic nematodes found in horses and donkeys worldwide. In this study, the complete mitochondrial genome of Cylicocyclus auriculatus Looss 1900, a representative member of this genus from the donkey in Southwest China was determined using the next-generation DNA sequencing technology. The genome was 13,851 bp in size and consisted of 36 genes including 12 protein-coding genes ( atp 6, cox 1-3, cytb , nad 1-6 and nad 4L), 22 transfer RNA genes and two ribosomal RNA genes ( rrn L and rrn S), as well as two non-coding regions. Phylogenetic analysis showed that C. auriculatus and Cylicocyclus insigne Boulenger 1917 were closely related, and then both grouped with other congeneric species and formed a monophyletic relationship with either species of Cyathostomum , Coronocyclus , Cyathostomum , Cylicostephanus or Cylicodontophorus , demonstrating their phylogenetic stability within Cyathostominae. These cumulative mitochondrial DNA data provide novel and useful genetic markers for molecular diagnostic, systematic and evolutionary biological studies of Cyathostominae nematodes.

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Characterization of the complete mitochondrial genomes of Coronocyclus labiatus and Cylicodontophorus bicoronatus: Comparison with Strongylidae species and phylogenetic implication

Cited by 5 publications

References 34 publications

Characterization of the mitochondrial genome of Tetrameres grusi and insights into the phylogeny of Spirurina

Characterization of the mitochondrial genome of Tetrameres grusi and insights into the phylogeny of Spirurina

The Mitochondrial Genome of Cylicocyclus elongatus (Strongylida: Strongylidae) and Its Comparative Analysis with Other Cylicocyclus Species

Complete mitochondrial genome of cylicocyclus auriculatus: molecular structure and phylogenetic analysis

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