Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway

Xu, Dian-Peng; Sung, Shi-Jean S.; Loboda, T.; Kormanik, Paul P.; Black, Clanton C.

doi:10.1104/pp.90.2.635

Cited by 95 publications

(59 citation statements)

References 25 publications

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“…It is the predominant sucrose cleavage enzyme in cereal endosperm and in storage organs such as tubers, fruits, seed or immature leaves [24,28]. Sucrose synthase in these carbohydrate sink organs provides substrates for respiration [29], 586 R. Le Hir et al…”

Section: Introductionmentioning

confidence: 99%

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Hir¹,

Pelleschi-Travier²,

Viémont³

et al. 2005

Ann. For. Sci.

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-In the search for a trophic control of rhythmic growth of young oak seedlings, sucrose synthase accumulation, localization and activity were studied in the apex, underlying internodes and leaves, during the second flush of shoot growth. The use of an anti-SuSy antibody raised against the Vicia faba protein allowed the detection of SuSy, which showed a 90 kDa subunit. This antibody, used to immunolocalize the SuSy protein in oak revealed a positive signal in the reserve parenchyma tissues of the apex and in the leaf vascular tissues and underlying internodes. The study of SuSy activity along with the growth pattern of the different organs, suggested that SuSy may be involved in the control of rhythmic growth through the mobilization of sucrose in storage tissues and through loading/unloading processes. Such activities would help to bring enough nutrients to support the high morphogenic and growth processes during the rhythmic growth of the shoot.common oak / rhythmic growth / bud / sugar metabolism / sink strength Résumé -Localisation et activité de la saccharose synthase au cours de la croissance rythmique de l'axe aérien du chêne pédonculé (Quercus robur L.). Afin d'évaluer l'existence d'un contrôle trophique dans la croissance rythmique, l'accumulation, la localisation et l'activité de la saccharose synthase ont été étudiées au sein de l'apex, de l'entre-noeud sous-jacent et des feuilles au cours de la deuxième vague de croissance chez le chêne pédonculé. L'utilisation d'un anticorps anti-SuSy de Vicia faba a permis de détecter la protéine SuSy chez le chêne, qui présente une sous-unité de 90 kDa. Cet anticorps a révélé un signal positif dans les parenchymes de réserves de l'apex et dans les tissus vasculaires des feuilles et des entre-noeuds sous-jacents. L'étude de l'activité SuSy au cours de la croissance des différents territoires suggère que cette enzyme pourrait être impliquée dans le contrôle de la croissance rythmique au travers de la mobilisation des réserves glucidiques et des processus de chargement/déchargement phloémien. Ces activités apporteraient l'énergie nécessaire aux processus de morphogenèse et de croissance observés durant la croissance rythmique de la tige. chêne pédonculé / croissance rythmique / bourgeon / métabolisme glucidique / force de puits

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Section: Introductionmentioning

confidence: 99%

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Hir¹,

Pelleschi-Travier²,

Viémont³

et al. 2005

Ann. For. Sci.

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“…The first hypothesis has been favored by several groups (2,8,24), who considered that the role of the reaction is to provide PPi Fru-2,6-P2, fructose 2,6-bisphosphate; Fru-6-P, fructose 6-phosphate; PPi, inorganic pyrophosphate; Glc-6-P, glucose 6-phosphate.…”

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confidence: 99%

Induction of Pyrophosphate:Fructose 6-Phosphate 1-Phosphotransferase by Anoxia in Rice Seedlings

Mertens¹,

Larondelle²,

Hers³

1990

Plant Physiol.

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Rice (Oryza sativa) seeds were imbibed for 3 days and the seedlings were further incubated for 8 days in the presence of either air or nitrogen. In aerobiosis, the specific activity of pyrophosphate:fructose 6-phosphate 1-phosphotransferase and that of the ATP-dependent phosphofructokinase increased about fourfold. In anaerobiosis, the specific activity of ATP-dependent phosphofructokinase remained stable, whereas that of pyrophosphate:fructose 6-phosphate 1-phosphotransferase increased as much as in the presence of oxygen and there was also a fourfold increase in the concentration of fructose 2,6-bisphosphate, a potent stimulator of that enzyme. These data suggest a preferential involvement of pyrophosphate:fructose 6-phosphate 1-phosphotransferase rather than of ATP-dependent phosphofructokinase in glycolysis during anaerobiosis.

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“…Inorganic nitrogen could do this by diverting triose phosphates out of the pentose phosphate reductive cycle, thus exercising substrate limitation for starch and sucrose synthesis. A second possibility is that one or more enzymes involved in starch and/or sucrose biosynthesis or its degradation are influenced by N2 fixation and/or inorganic nitrogen supply (17,31,38) (CC Black, private communication). For example, Kerr et al (17) found that the activity of ADPG-PPiase, the principal rate limiting starch synthesis pathway enzyme, was slightly lower in soybean leaves of NO3-fed plants compared to leaves from N2-fixing plants.…”

Section: Maintenance Of Photosynthetic Rate In Nod+/-source Leaves Tomentioning

confidence: 99%

Photosynthesis and Photosynthate Partitioning in N₂-Fixing Soybeans

Veau¹,

Robinson²,

Warmbrodt³

et al. 1990

Plant Physiol.

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Leaf area, chlorophyll content, net CO2 photoassimilation, and the partitioning of fixed carbon between leaf sucrose and starch and soluble protein were examined in Glycine max (L) Merr Legumes have the capability to acquire nitrogen for growth through N2 fixation and inorganic nitrogen metabolism, and reduced carbon is needed by the plants to carry out both processes. Little is known about the effects of N2 fixation versus inorganic nitrogen metabolism on photosynthesis and photosynthate partitioning within the source leaves of the host plants of legume-Rhizobium symbioses, although the influence of photosynthesis and photosynthate production of the source leaves upon N2 fixation in legumes is well documented (1, 12, 13, 34). As two distinct nitrogen acquiring processes, N2 fixation and inorganic nitrogen metabolism probably generate two different sink strengths for reduced carbon (29). There is evidence that because of the maintenance of the bacteria and the nodules, N2 fixation generates 259 the greater sink for reduced carbon (6, 21, 23). However, this is not entirely certain (7).It is known that sink strength influences photosynthetic metabolism and photosynthate partitioning (24, 33). High sink strength elevates net photosynthetic rates (14, 33). If N2 fixation is the stronger sink, then N2-fixing legumes should have higher photosynthetic rates than non-N2-fixing counterparts. This laboratory has established that in some cases N2-fixing soybeans have higher leaf net photosynthesis than non-N2-fixing plants (27). This higher photosynthetic rate was evident in both intact trifoliolates and isolated mesophyll cells. However, others have shown that leafnet photosynthesis between the two groups ofplants are not significantly different (2), but this point needs further investigation.Reduced carbon is partitioned within a plant to meet various metabolic, storage, and structural demands. Photosynthate partitioning can be thought ofin terms ofthe amount of carbon transported out of the photosynthetic cells to meet the demands of nonphotosynthetic tissue versus that amount of carbon sequestered for later use. Photosynthate is stored usually in the form of starch or some other carbohydrate. The amount ofcarbon diverted to meet the metabolic needs versus starch storage is influenced by the strength of the sink. High sink strength will result in more photosynthate from the source leaves being synthesized for transport and less for storage (24). Source leaves from N2-fixing plants should have lower foliar starch levels than source leaves of plants assimilating inorganic nitrogen if the demands of N2 fixation for fixed carbon are greater than for inorganic nitrogen assimilation.A lower foliar starch content within nodulated legumes has been shown, indicating that N2 fixation is a stronger sink than inorganic nitrogen assimilation (16). However, there have been reports of N2-fixing soybeans having higher starch content within source leaves than non-N2-fixing soybeans (2,3,17). This suggests that nitrogen assimilat...

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Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway

Abstract: The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dqpendent pathway was biochemically characterized using soluble extracts from several plants.

Cited by 95 publications

References 25 publications

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Induction of Pyrophosphate:Fructose 6-Phosphate 1-Phosphotransferase by Anoxia in Rice Seedlings

Photosynthesis and Photosynthate Partitioning in N₂-Fixing Soybeans

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Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway

Abstract: The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dqpendent pathway was biochemically characterized using soluble extracts from several plants.

Cited by 95 publications

References 25 publications

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Induction of Pyrophosphate:Fructose 6-Phosphate 1-Phosphotransferase by Anoxia in Rice Seedlings

Photosynthesis and Photosynthate Partitioning in N2-Fixing Soybeans

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Product

Resources

About

Photosynthesis and Photosynthate Partitioning in N₂-Fixing Soybeans