Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil

Silva, Tatiane; Nogueira, Paulo Afonso; Magalhães, Gleiciene Félix; Grava, Andréa Fagundes; Silva, Luiz Hildebrando Pereira da; Orlandi, Patrícia Puccinelli

doi:10.1590/s0074-02762008000700017

Cited by 19 publications

(24 citation statements)

References 13 publications

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“…The data shown in this study are consistent with data 4,13,17,18 shown for other states of Brazil and other countries from Central America, North America, Africa, Middle East 19 and India as described by Drews et al, 2010 .…”

Section: Discussionsupporting

confidence: 93%

Caracterização da resistência antimicrobiana de amostras de Shigella spp. isoladas em Belém, Estado do Pará, Brasil (1990-2000)

Bastos¹,

Loureiro²

2010

Rev Pan-Amaz Saude

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Section: Discussionsupporting

confidence: 93%

Caracterização da resistência antimicrobiana de amostras de Shigella spp. isoladas em Belém, Estado do Pará, Brasil (1990-2000)

Bastos¹,

Loureiro²

2010

Rev Pan-Amaz Saude

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“…En la presente investigación se observó que la especie más común resultó ser S. flexneri, seguido por S. sonnei y S. boydii y finalmente no se reportó presencia de S. dysenteriae. Estos datos son revalidados con estudios en Latinoamérica, especialmente en Brasil, Uruguay, Cuba y Argentina donde la información para la prevalencia de estas especies de Shigella son similares [19][20][21][22].…”

Section: Disusiónunclassified

“…Durante años las enfermedades diarreicas como la shigelosis han sido asistidas con el uso de antibióticos como trimetoprim /sulfametoxazol, ampicilina, ciprofloxacina y azitromicina [22]; sin embargo, la aparición de aislados multirresistentes ha limitado el tratamiento y ha incrementado la prevalencia de la enfermedad [24]. La diversidad de fenotipos de resistencia mostrados por los aislados pudiera deberse a que la resistencia está siendo mediada por plásmidos, con la excepción para quinolonas y azitromicina, y además por integrones o transposones que estén incorporados al cromosoma bacteriano [23].…”

Section: Disusiónunclassified

“…Estudios competentes relacionados a la resistencia a antimicrobianos en el patotipo Shigellae en países de Latinoamérica como Brasil, Chile, Cuba, Argentina, y Uruguay reportan una alta resistencia a los antibióticos tri- metoprim/sulfametoxazol, cloranfenicol y ampicilina [19,21,22,25,26]. Mientras que para países como Canadá, Senegal e India se reporta una resistencia a ampicilina y tetraciclina [27].…”

Section: Disusiónunclassified

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Sensibilidad antimicrobiana entre los serogrupos de <em>Shigellae</em> aislados en la ciudad de Quito-Ecuador

Granda

Alcocer

2015

Av. Cienc. Ing. (Quito)

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The pathotype Shigellae includes bacillus from the species S. flexneri, S. sonnei, S. boydii and S. dysenteriae which are causatives of the disease known as bacillary dysentery or shigellosis. The pathogenicity of the genus is characterized by the presence of high antibiotic resistance. The aim of this study was to identify the species and antimicrobial susceptibility in clinical isolates of Shigellae. We analyzed 79 isolates obtained from Zurita & Zurita Laboratorios and Vozandes Hospital in the city of Quito. Three species were obtained by serotyping, using polyclonal antisera: S. flexneri (n=50; 63,29 %), S. sonnei (n=23; 29,11 %), and S. boydii (n=6; 7,59 %). The antimicrobial susceptibility was analyzed using the Kirby Bauer method and recommendations from the "Clinical and Laboratory Standards Institute" (CLSI). Most of the isolates showed resistance to tetracycline (n=76; 96,20 %), ampicillin (n=75; 94,94 %), trimethoprim/sulfamethoxazole (n= 68; 86,08 %) and chloramphenicol (n=67; 84,81 %). The results showed the incidence of multidrug resistance to antibiotics commonly used for treating shigellosis, and the presence of three species of Shigellae.Keywords. Antibiotics, bacillary dysentery, Shigellae. ResumenEl patotipo Shigellae comprende bacilos de las especies S. flexneri, S. sonnei, S. boydii y S. dysenteriae, causantes de la enfermedad conocida como disentería bacilar o shigelosis. La patogenicidad del género se caracteriza por la presencia de una alta multirresistencia a antibióticos. El objetivo de este estudio fue identificar las especies y la sensibilidad a antimicrobianos en aislados clínicos de Shigellae. Se analizaron 79 aislados obtenidos de Zurita & Zurita Laboratorios y del Hospital Vozandes en la ciudad de Quito. Mediante serotipaje, con el uso de antisueros policlonales, se obtuvieron 3 especies: S. flexneri (n=50; 63,29 %), S. sonnei (n= 23; 29,11 %), y S. boydii (n= 6; 7,59 %). La sensibilidad antimicrobiana se analizó siguiendo el método de Kirby-Bauer y las recomendaciones del "Clinical and Laboratory Standards Institute" (CLSI). La mayoría de las cepas mostraron resistencia a tetraciclina (n=76; 96,20 %), ampicilina (n=75; 94,94 %), trimetoprim/sulfametoxazol (n=68; 86,08 %) y cloranfenicol (n=67; 84,81 %). Los resultados obtenidos demostraron la incidencia de multirresistencia a antibióticos comúnmente utilizados para el tratamiento de shigelosis, y la presencia de 3 especies de Shigellae.Palabras Clave. Antibióticos, disentería bacilar, Shigellae. IntroducciónEl patotipo Shigellae comprende bacilos Gram negativos de 0,3 a 1 µm diámetro y de 1 a 6 µm de longitud, no móviles, no formadores de esporas, anaerobios facultativos, pertenecientes a la familia Enterobacteriaceae y causantes de la enfermedad conocida como shigelosis o disentería bacilar [1]. Su identificación se fundamenta en características bioquímicas y antigénicas, en base a ellas se describen cuatro especies: Shigella dysenteriae, serogrupo A; Shigella flexneri, serogrupo B; Shigella boydii, serogrupo C; y, Shige...

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“…According to Silva et al (Silva et al, 2008), shigellosis is currently an important health problem around the world, occurring predominantly in children younger than five years old, mainly in developing countries. Shigellosis is caused by Shigella spp., including S. dysenteriae, S. flexneri, S. boydii and S. sonnei (Kuo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Pal¹

2014

ILNS

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Diarrhoea in developing countries is caused by an increasingly long list of bacterial, viral, and parasitic pathogens with rotavirus, Enterotoxigenic Escherichia coli, Campylobacter, Shigella, and Salmonella heading the list. Using methods to detect most of the known enteropathogens, one or more enteropathogen(s) is isolated in two-thirds of diarrhoeal illnesses in the developing world. Deoxyribonucleic acid probes have proved very useful in detecting pathogens such as enterotoxigenic (ETEC), enteroinvasive (EIEC), enteropathogenic E. coli (EPEC), and Shigella but have not yet proved to be particularly rapid or less expensive. Molecular biology has proved useful in epidemiological studies as a means of strain identification and detection of genome diversity. Since the introduction of ribonucleic acid gene restriction patterns as taxonomic tools in 1986, ribotyping has become an established method for systematics, epidemiological, ecological population and genome diversity studies of microorganisms including Shigella. The technological development culminated in the automation of ribotyping which allowed for high-throughput applications. PCR ribotyping has proved being a highly discriminatory, flexible, robust and cost-efficient routine technique which makes inter-laboratory comparison and build of ribotype databases possible, too. The aim of the present review is to determine the present status of ribotyping technique in detecting the diversity in Shigella isolates.

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Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil

Abstract: The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil)

Cited by 19 publications

References 13 publications

Caracterização da resistência antimicrobiana de amostras de Shigella spp. isoladas em Belém, Estado do Pará, Brasil (1990-2000)

Caracterização da resistência antimicrobiana de amostras de Shigella spp. isoladas em Belém, Estado do Pará, Brasil (1990-2000)

Sensibilidad antimicrobiana entre los serogrupos de <em>Shigellae</em> aislados en la ciudad de Quito-Ecuador

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

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