Characterization of Salmonella Enterica Isolated from Poultry Hatcheries and Commercial Broiler Chickens

Shehata, Awad A.

doi:10.29261/pakvetj/2019.033

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…These results are in agreement with other studies [36][37]. These findings support the assumption that there is a difference in the occurrence of some salmonella types at young ages versus older ages [38][39][40]. The most commonly isolated serotype from different organs was S. Enteritidis which is consistent with results recorded in Egypt [41].…”

Section: Discussionsupporting

confidence: 93%

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Roshdy

Mohamed

Elsebaey³

et al. 2020

Zagazig Veterinary Journal

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Salmonella species are causing high morbidity and mortality rates in broiler chickens and other birds particularly young ages resulting in great economic losses. The present study was performed to detect the effect of age and season on isolation rates of Salmonella species from 120 examined broiler chickens with different ages (1-7 days-old and more than 7days-old) in different seasons (summer, winter, autumn and spring). The highest isolation rates of Salmonella species were obtained from broilers of 1-7 daysold in summer (40%) followed by broilers of more than 7 days old in summer (33.3%). The increased isolation rates of Salmonella species was detected from liver of broilers of both ages (1-7 and more than 7 days old). Seventeen Salmonella isolates were obtained in this study from both ages. Serotyping of these Salmonella isolates revealed S. Enteritidis (5 isolates), S. Typhimurium (3 isolates), S. Infantis, S. Bargny, S. Newport, S. Magherafelt (2 isolates) and finally S. Apeyeme in (1 isolate). The most frequent resistance phenotypes were tetracycline (70%-71.4%), from 1-7 days-old and more than 7 days-old chicks and finally ampicillin (60%, 57.1%). From the result obtained by using VITEK2 system, the highest resistance was recorded against Tetracycline in all Salmonella species. All Salmonella isolates showed-associated genes. The invA gene was identified in all Salmonella serovars isolated from chickens of the two age groups, whereas, avrA gene was detected in all the isolates except S. Magherafelt recovered from the age group of more than 7 days-old birds. The tetracycline resistance gene (tetA) was identified in all the examined isolates.

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Section: Discussionsupporting

confidence: 93%

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Roshdy

Mohamed

Elsebaey³

et al. 2020

Zagazig Veterinary Journal

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“…In this study, Salmonella was isolated from 128 out of 657 (19.5%) examined poultry farms in the period from 2014 to 2018, and these results were nearly [18,23] found Salmonella in low percentage, about 7% and 7.1%, respectively, in samples isolated from poultry farms in Egypt. Moreover, El-Sharkawy et al [24] isolated Salmonella in high percentage (41%) from poultry farms in Egypt; also, Salmonella was isolated from poultry farms in Brazil and USA in high percentage, about 37% and 56%, respectively [25,26].…”

Section: Salmonella Species Isolatesmentioning

confidence: 72%

Monitoring of Antimicrobial Susceptibility of Bacteria Isolated from Poultry Farms from 2014 to 2018

Hamed

Abdelaty

Sorour

et al. 2021

Veterinary Medicine International

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The current situation of antibiotic resistance of most bacterial pathogens was a threat to the poultry and public health with increasing economic losses. Regarding this problem, monitoring of the circulating microorganisms occurred with the antibiotic resistance profile. A total of 657 different samples from internal organs (liver, heart, lung, and yolk) and paper-lining chick boxes were collected from native chicken farms which were submitted to the Reference Laboratory for Veterinary Quality Control on Poultry Production in the period from 2014 to 2018 for the detection of Salmonella, Escherichia coli (E. coli), and Staphylococcus. The bacterial isolates were tested for their antimicrobial susceptibility by disk diffusion technique. Salmonella was isolated from 128 out of 657 (19.5%), E. coli was isolated from 496 out of 657 (75.5%), and Staphylococcus species was isolated from 497 out of 657 (75.6%). All Salmonella positive samples were examined for antibiotic resistance against 10 different antibiotics, and the highest percentage all over the five years was against penicillin, ampicillin, and tetracycline. All E. coli positive samples were examined for antibiotic resistance against 14 different antibiotics, and the highest percentage all over the five years was with ampicillin, tetracycline, norfloxacin, streptomycin, and danofloxacin. All Staphylococcus positive sample species were examined for antibiotic resistance against 14 different antibiotics, and the highest percentage of resistance all over the five years was shown with tetracycline, streptomycin, ampicillin, and nalidixic acid.

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“…This problem could limit the therapeutic options for infections, caused by antibiotic-resistant S . Enteritidis strains [ 6 , 20 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence, virulence factor and antimicrobial resistance analysis of Salmonella Enteritidis from poultry and egg samples in Iran

et al. 2021

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Background Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most common serovars, associated with human salmonellosis. The food-borne outbreak of this bacterium is mainly related to the consumption of contaminated poultry meat and poultry products, including eggs. Therefore, rapid and accurate detection, besides investigation of virulence characteristics and antimicrobial resistance profiles of S. Enteritidis in poultry and poultry egg samples is essential. A total of 3125 samples (2250 poultry and 875 poultry egg samples), sent to the administrative centers of veterinary microbiology laboratories in six provinces of Iran, were examined for Salmonella contamination, according to the ISO 6579 guideline. Next, duplex PCR was conducted on 250 presumptive Salmonella isolates to detect invA gene for identification of the genus Salmonella and sdf gene for identification of S. Enteritidis. Subsequently, the S. Enteritidis isolates were examined for detection of important virulence genes (pagC, cdtB, msgA, spaN, tolC, lpfC, and spvC) and determination of antibiotic resistance patterns against nalidixic acid, trimethoprim-sulfamethoxazole, cephalothin, ceftazidime, colistin sulfate, and kanamycin by the disk diffusion method. Results Overall, 8.7 and 2.3% of poultry samples and 6.3 and 1.3% of eggs were contaminated with Salmonella species and S. Enteritidis, respectively. The invA and msgA genes (100%) and cdtB gene (6.3%) had the highest and the lowest prevalence rates in S. Enteritidis isolates. The spvC gene, which is mainly located on the Salmonella virulence plasmid, was detected in 50.8% of S. Enteritidis isolates. The S. Enteritidis isolates showed the highest and the lowest resistance to nalidixic acid (87.3%) and ceftazidime (11.1%), respectively. Unfortunately, 27.0% of S. Enteritidis isolates were multidrug-resistant (MDR). Conclusion The rate of contamination with Salmonella in the poultry and egg samples, besides the presence of antimicrobial resistant and MDR Salmonella isolates harboring the virulence genes in these samples, could significantly affect food safety and subsequently, human health. Therefore, continuous monitoring of animal-source foods, enhancement of poultry farm control measures, and limiting the use of antibiotics for prophylactic purposes in food producing animals, are essential for reducing the zoonotic risk of this foodborne pathogen for consumers and also choosing effective antibiotics for the treatment of salmonellosis.

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Characterization of Salmonella Enterica Isolated from Poultry Hatcheries and Commercial Broiler Chickens

Cited by 11 publications

References 25 publications

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Monitoring of Antimicrobial Susceptibility of Bacteria Isolated from Poultry Farms from 2014 to 2018

Prevalence, virulence factor and antimicrobial resistance analysis of Salmonella Enteritidis from poultry and egg samples in Iran

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