Characterization of procyanidin extracts from hawthorn (Crataegus pinnatifida) in human colorectal adenocarcinoma cell line Caco-2, simulated Digestion, and fermentation identified unique and novel prebiotic properties

Han, Xue; Zhou, Qian; Gao, Zhe; Xu, Guanying Bianca; Chen, Hong; Chitrakar, Bimal; Sun, Ya-Sai; Zhao, Wen; Lin, Xuan; Zhou, Kaixuan; Zhang, Zhisheng

doi:10.1016/j.foodres.2022.112393

Cited by 11 publications

(5 citation statements)

References 49 publications

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“…Numerous factors should be considered when exploring the role of gut microbiota in the synergistic anti-obesity effects of CP and WE. The bioactive constituents in CP and WE have beneficial effects on gut microbiota [ 46 , 47 , 48 , 49 , 50 ]. For instance, the procyanidin extract of CP down-regulated the abundance of Parabacteroides and up-regulated Turicibacter during in vitro digestion and fermentation [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…The bioactive constituents in CP and WE have beneficial effects on gut microbiota [ 46 , 47 , 48 , 49 , 50 ]. For instance, the procyanidin extract of CP down-regulated the abundance of Parabacteroides and up-regulated Turicibacter during in vitro digestion and fermentation [ 49 ]. Zou et al reported that WE and its polysaccharide fractions remarkably decreased the abundance of Parabacteroides and Blautia while increasing the abundance of Allobaculum [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Crataegus pinnatifida and Wolfiporia extensa Combination on Diet-Induced Obesity and Gut Microbiota

Yuan,

Hu,

Yang

et al. 2024

Foods

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Obesity is a multifactorial chronic metabolic disease with multiple complications. Crataegus pinnatifida (CP) and Wolfiporia extensa (WE) are traditional functional foods with improving metabolic health properties. This study demonstrated the effect of CP and WE combination on ameliorating obesity induced by a high-fat diet (HFD). Moreover, the CP-WE food pair ameliorated HFD-induced metabolic disorders, including glucose intolerance, insulin resistance, hyperlipidemia, and hepatic steatosis. 16S rRNA gene amplicon sequencing and analysis revealed that CP combined with WE reshaped the composition of gut microbiota in HFD-fed mice. Furthermore, correlation analysis revealed a substantial association between the obesity-related parameters and the shifts in predominant bacterial genera influenced by the food pair intervention. In conclusion, this study demonstrated that the CP-WE food pair ameliorated HFD-induced obesity and reshaped gut microbiota composition, providing a promising approach to combat obesity through specific food combinations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effects of Crataegus pinnatifida and Wolfiporia extensa Combination on Diet-Induced Obesity and Gut Microbiota

Yuan,

Hu,

Yang

et al. 2024

Foods

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“…To determine the cytotoxicity of 7,8‐DHF for transport experiments, a CCK‐8 assay was carried out (Han et al, 2023 ). A total of 200 μL culture medium containing Caco‐2 cells at a density of 3 × 10 4 cells/mL was seeded in a 96‐well plate and incubated for 24 h to allow for adherence.…”

Section: Methodsmentioning

confidence: 99%

Impact of dietary plant flavonoids on 7,8‐dihydroxyflavone transepithelial transport in human intestinal Caco‐2 cells

Chen,

Xia,

Wang

et al. 2023

Food Science & Nutrition

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Abstract7,8‐dihydroxyflavone (7,8‐DHF) is a biologically active flavone with various physiological activities, including neuroprotection, anti‐inflammation, and weight loss. Previous studies have found that the efflux protein P‐glycoprotein (P‐gp) significantly affects the transepithelial transport of 7,8‐DHF in the intestine, resulting in its low oral bioavailability. Based on this, in this study, a Caco‐2 monolayer cell model was used to investigate 14 dietary plant flavonoids as potential P‐gp inhibitors, and their effects on the transepithelial transport and in vitro digestion of 7,8‐DHF were explored. The results showed that among the 14 plant flavonoids, hesperetin, epigallocatechin gallate, fisetin, kaempferol, quercetin, and isoorientin increased and the apparent permeability coefficients (Papp) of 7,8‐DHF at AP → BL direction and lowered Papp value at BL → AP direction to varying degrees, reducing the efflux ratio of 7,8‐DHF less than 1.5. In particular, kaempferol and quercetin exhibited the best effect on promoting the transepithelial transport of 7,8‐DHF, especially when used at molar concentration ratios of 1:1 and 1:2 with 7,8‐DHF. This is beneficial for improving the oral bioavailability of 7,8‐DHF. Meanwhile, 7,8‐DHF was found to maintain structural stability in simulated saliva, gastric juice, and intestinal juice, and its stability was not affected by the coexistence of quercetin and kaempferol. Overall, this study provided a theoretical basis for seeking natural and safe P‐gp inhibitors to improve the oral absorption of natural products.

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“…Through LC-ESI-MS analysis, our earlier findings showed that HPOE primarily consists of epicatechin and proanthocyanidins B2, B5, and C1 [33]. The structural information and related targets of these active components were determined through the TCMSP (https://tcmsp-e.com/) (accessed on 19…”

Section: Target Screening Of Hpoementioning

confidence: 99%

Hawthorn Proanthocyanidin Extract Inhibits Colorectal Carcinoma Metastasis by Targeting the Epithelial-Mesenchymal Transition Process and Wnt/β-Catenin Signaling Pathway

Wang,

Sun,

et al. 2024

Foods

Self Cite

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Colorectal carcinoma (CRC) is a major global health concern, with cancer metastasis being the main cause of patient mortality, and current CRC treatments are challenged by drug resistance. Although natural compounds, especially in foods like hawthorn proanthocyanidin extract (HPOE), have good anticancer activity, their effects on CRC metastasis remain unknown. Therefore, our objective was to investigate the impact and potential mechanisms of HPOE on the movement and infiltration of cells in the HCT116 CRC cells. Firstly, scratch-healing experiments confirmed the anti-migratory and anti-invasive capabilities of HPOE. Then, network pharmacology identified 16 possible targets, including MMP-9. Subsequently, RT-qPCR and Western blotting experiments confirmed that HPOE downregulated epithelial-mesenchymal transition-related factors (N-cadherin and MMP-9) and inhibited Wnt/β-catenin pathway activation. Finally, these results were experimentally validated using the Wnt pathway activator Licl and inhibitor XAV939. It was confirmed that HPOE had a certain inhibitory effect on the activation of the Wnt signaling pathway caused by the activator Licl and could enhance the inhibitory effect of the inhibitor XAV939. Our findings provide a basis for developing functional foods or dietary supplements, especially positioning HPOE as a functional food raw material for adjuvant treatment of CRC, given its ability to inhibit metastasis through the Wnt/β-catenin pathway.

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Characterization of procyanidin extracts from hawthorn (Crataegus pinnatifida) in human colorectal adenocarcinoma cell line Caco-2, simulated Digestion, and fermentation identified unique and novel prebiotic properties

Cited by 11 publications

References 49 publications

The Effects of Crataegus pinnatifida and Wolfiporia extensa Combination on Diet-Induced Obesity and Gut Microbiota

The Effects of Crataegus pinnatifida and Wolfiporia extensa Combination on Diet-Induced Obesity and Gut Microbiota

Impact of dietary plant flavonoids on 7,8‐dihydroxyflavone transepithelial transport in human intestinal Caco‐2 cells

Hawthorn Proanthocyanidin Extract Inhibits Colorectal Carcinoma Metastasis by Targeting the Epithelial-Mesenchymal Transition Process and Wnt/β-Catenin Signaling Pathway

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