Characterization of probiotic strains: An application as feed additives in poultry against Campylobacter jejuni

Santini, Cecilia; Baffoni, Loredana; Gaggìa, Francesca; Granata, Marta; Gasbarri, Rossana; Gioia, Diana Di; Biavati, Bruno

doi:10.1016/j.ijfoodmicro.2010.03.039

Cited by 176 publications

(140 citation statements)

References 56 publications

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“…It has been suggested that bifidobacteria excrete an anti-microbial substance that affects Campylobacter and other bacteria (Gibson and Wang, 1994). Furthermore, administration of Bifidobacteria longum to poultry as a probiotic for 2 weeks was shown to reduce their C. jejuni concentration within that period (Santini et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

Jensen

Mejer

Mølbak

et al. 2011

Animal

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The restrictions on the use of antibiotic and anthelmintic treatments in organic pig farming necessitate alternative non-medical control strategies. Therefore, the antibiotic and parasite-reducing effect of a fructan-rich (prebiotic) diet of dried chicory was investigated in free-ranging piglets. Approximately half of 67 piglets from nine litters were experimentally infected with Ascaris suum and Trichuris suis in the suckling period (1 to 7 weeks of age) and 58 of the piglets were challenged daily with Eschericia coli O138:F8 for 9 days after weaning to induce weaning diarrhoea. The litters were fed either chicory (30% dry matter) or a control diet. The effect of chicory on intestinal helminths, intestinal microbiota, especially Bifidobacteria and Campylobacter spp. and E. coli post-weaning diarrhoea was assessed. The weight gain of the piglets was not impaired significantly by chicory. The intestinal A. suum worm burden was reduced by 64% ( P 5 0.034) in the chicory-fed piglets, whereas these same piglets had 63% more T. suis worms ( P 5 0.016). Feeding with chicory elicited no changes among the main bacterial groups in ileum according to terminal restriction fragment length polymorphism analysis. However, the terminal-restriction fragment (T-RF) 208 bp, which may belong to Lachnospiraceae, was stimulated by the chicory feed ( P 5 0.03), and T-RF 370 bp that matches Enterobacter belonging to the Enterobacteria was reduced (P 5 0.004). In addition, chicory increased the level of Bifidobacteria ( P 5 0.001) and the faecal Campylobacter excretion level was transitorily reduced in chicory-fed piglets at 7 weeks of age ( P 5 0.029). Unfortunately, it was not possible to assess the effect of chicory on post-weaning diarrhoea as it did not develop. In conclusion, feeding piglets chicory around the time of weaning caused complex changes of the microbiota and parasite communities within the intestinal tract, and feeding piglets chicory may therefore serve as an animal-friendly strategy to control pathogens.

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Section: Discussionmentioning

confidence: 99%

The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

Jensen

Mejer

Mølbak

et al. 2011

Animal

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“…Antibiotic resistances of the isolates were tested as described by Chaiyawan et al (2010) and Santini et al (2010) with modifications. Ampicillin, kanamycin, chloramphenicol, erythromycin, vancomycin, neomycin, ciprofloxacin, streptomycin, and gentamicin were the antibiotics tested.…”

Section: Antibiotic Susceptibilitymentioning

confidence: 99%

“…As such, there has been an increasing concern about non-therapeutic uses of antibiotics, ultimately culminating in bans of their use as animal feed additives. The European Parliament and the Council of the European Union have encouraged the development of alternative products to replace antibiotics as feed supplements for growth promotion (Santini et al 2010; The European Parliament and the Council of the European Union 2003). Consequently, there is an ongoing search for replacement products capable of enhancing growth and preventing disease (Chaiyawan et al 2010;Santini et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, there is an ongoing search for replacement products capable of enhancing growth and preventing disease (Chaiyawan et al 2010;Santini et al 2010). An effective and safe alternative to antibiotic implementation is the use of probiotics, which protect the animal from pathogens by improving the microbial balance in the gastrointestinal tract to exclude potentially harmful bacteria (Chaiyawan et al 2010;Modesto et al 2009;Patterson and Burkholder 2003;Santini et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Screening and selection of novel animal probiotics isolated from bovine chyme

et al. 2012

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Probiotics, gut-colonizing microorganisms capable of conferring a number of health benefits to their hosts, are highly desirable as animal feed supplements. Members of the Gram-positive genus Bacillus are often utilized as probiotics, since endospores formed by those bacteria render them highly resistant to environmental extremes and therefore capable of surviving gastrointestinal tract conditions. In this study, 84 distinct bacterial colonies were obtained from bovine chyme and 29 isolates were determined as Bacillus species. These isolates were principally screened for their antimicrobial activity against a group of two Gram-positive and four Gram-negative bacteria, including known human and animal pathogens such as Salmonella enterica, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Seven strains displaying strong antimicrobial activity against the test cohort were further evaluated for other properties desirable from animal probiotics, including high spore-forming capacity and adhesiveness, resistance to pH extremes and ability to form biofilms. The isolates were found to resist simulated gastrointestinal conditions and most of the antibiotics tested. In addition, plasmid presence was checked and cytotoxicity tests were performed to evaluate the potential risks of antibiotic resistance transfer and unintended pathogenic effects on host, respectively. We propose that the bacterial isolates are suitable for use as animal probiotics.

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“…The live bacteria in probiotics affect the host animal beneficially via improving the intestinal microbial balance [4] . Likewise, they inhibit the growth of pathogenic microorganisms by colonial formation [5] . Probiotics might enhance the permeability of epithelium, increase the phagocytosis and strengthen the non-specific immunity [6] , and increase the feed efficiency by changing the intestinal microflora [7] .…”

Section: Introductionmentioning

confidence: 99%

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Yenice¹,

Çelebi²,

Yörük³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryIn the current study, the effect of kefir upon the performance, intestinal microflora and histopathology of certain organs in laying hens was investigated. Totally, 108 Lohmann Brown layers, aged 24 weeks, were allocated randomly into three groups, as; Group C (control, n=36): no treatment, Group A (n=36): 10 cc, and Group B (n=36): 7.5 cc kefir per litre of water. Animals were fed for 10 weeks with basal diets. Livers showed moderate level of hydropic degeneration, some lipidosis and focal haemorrhages with high amounts of kefir (Group A). Fewer active follicles in the ovarium were also observed in this group. The egg yield was significantly (P<0.01) lower in Group A (89.40±0.91) than in Group C (92.50±0.83) and Group B (92.86±0.87). For the pH of large intestines, unlike the small ones, it was significantly changed (P<0.01) from basic to acidic milieu in kefir-treated groups. The titres of coliform (E. coli), aerobic (Lactobacillus spp.), and anaerobic bacteria (Peptostreptococcus spp.) were significantly decreased (P<0.05 to P<0.001) with increased intake for both intestinal tracts. We conclude that; i) high kefir intake could unfavourably impair the digestive organ structures, ii) the supplementations led to a marked decrease in the large intestinal pH and microbiological load of the intestines, and iii) high kefir level markedly decreased the egg yield, unlike the low concentration as leading to a considerable improvement from the 6 th weeks onwards. Keywords: Kefir, Laying hen, Microbiology, Pathology, Probiotic Yumurtacı Tavuklarda Kefirin Performans, Barsak Mikroflorası ve Bazı Organların Histopatolojisi Üzerine Etkisi ÖzetMevcut araştırmada, yumurtacı tavuklarda kefirin performans, barsak mikroflorası ve bazı organların histopatolojisi üzerine etkisi araştırıldı. 24 haftalık toplam 108 adet Lohmann Brown yumurtacı tavuk; Grup K (kontrol, n=36), Grup A (n=36): 10 cc ve Grup B (n=36): 7.5 cc kefir/L su olarak rastgele 3 gruba ayrıldı. Hayvanlar 10 hafta süreyle bazal rasyonla beslendi. Yüksek kefir miktarı (Grup A), karaciğerlerde orta düzey hidropik dejenerasyon, belli düzeyde lipidosis ve fokal hemorajiler oluşturdu. Ayrıca, bu grupta ovaryumdaki aktif follikül sayısının daha az olduğu gözlendi. Grup A'daki yumurta verimi (89.40±0.91), Grup C (92.50±0.83) ve Grup B'dekinden (92.86±0.87) önemli düzeyde (P<0.01) daha düşük bulundu. Kefir uygulanan gruplardaki kalın bağırsak pH'sı, ince bağırsakların aksine, bazikten asidik ortama doğru önemli düzeyde (P<0.01) değişti. Koliform (E. coli), aerobik (Lactobacillus spp.), ve anaerobik bakteri (Peptostreptococcus spp.) titreleri artan probiyotik alımıyla birlikte önemli düzeyde (P<0.05 -P<0.001) azaldı. Sonuç olarak; i) yüksek kefir alımının sindirim organı yapılarını olumsuz yönde etkileyebildiği, ii) katkıların kalın bağırsak pH'sını ve bağırsakların mikrobiyolojik yükünü önemli düzeyde azalttığı, ve iii) yüksek kefir düzeyinin yumurta verimini önemli düzeyde azaltmasına karşın, düşük konsantrasyonun 6. haftadan sonra belli oranda artırdığı kanısına va...

show abstract

Characterization of probiotic strains: An application as feed additives in poultry against Campylobacter jejuni

Cited by 176 publications

References 56 publications

The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

Screening and selection of novel animal probiotics isolated from bovine chyme

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

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