Characterization of Polymeric Solutions as Injectable Vehicles for Hydroxyapatite Microspheres

Oliveira, Serafim M.; Almeida, Isabel F.; Costa, Paulo; Barrias, Cristina C.; Ferreira, M. Rosa Pena; Bahia, Maria Fernanda; Barbosa, Mário A.

doi:10.1208/s12249-010-9447-3

Cited by 18 publications

(12 citation statements)

References 31 publications

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“…The injectability of the produced biocomposites resulted in accordance with other injectable systems found in the literature [52,53]. The injection of pectin biocomposites through the 30 G needle appeared difficult for the softer formulations and not possible for those formulations with highest amounts of pectin and hydroxyapatite.…”

Section: Discussionsupporting

confidence: 83%

Reactive hydroxyapatite fillers for pectin biocomposites

Munarin

Petrini

Barcellona³

et al. 2014

Materials Science and Engineering: C

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In this work, a novel injectable biocomposite hydrogel is produced by internal gelation, using pectin as organic matrix and hydroxyapatite either as crosslinking agent and inorganic reinforcement. Tunable gelling kinetics and rheological properties are obtained varying the hydrogels' composition, with the final aim of developing systems for cell immobilization. The reversibility by dissolution of pectin-hydroxyapatite hydrogels is achieved with saline solutions, to possibly accelerate the release of the cells or active agents immobilized. Texture analysis confirms the possibility of extruding the biocomposites from needles with diameters from 20 G to 30 G, indicating that they can be implanted with minimally-invasive approaches, minimizing the pain during injection and the side effects of the open surgery. L929 fibroblasts entrapped in the hydrogels survive to the immobilization procedure and exhibit high cell viability. On the overall, these systems result to be suitable supports for the immobilization of cells for tissue regeneration applications.

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Section: Discussionsupporting

confidence: 83%

Reactive hydroxyapatite fillers for pectin biocomposites

Munarin

Petrini

Barcellona³

et al. 2014

Materials Science and Engineering: C

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“…Steady increases in the viscosity changed the rheology nature of the alginate solution and this finding was clearly observed at a shear rate of 45/s (data not shown). It shows that an increasing viscosity of alginate solutions was reducing shear stress and was in agreement with the work by Oliveira et al (2010).…”

Section: Properties Of the Alginate Solutionssupporting

confidence: 91%

Preparation and Characterization of Alginate Beads by Drop Weight

Kamaruddin¹,

Yusoff²,

Aziz³

2014

IJTech

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The preparation and characterization of macro alginate beads are always associated with appropriate techniques involving precise measurement of shape, size, volume and density of the products. Depending on the type of application, encapsulation of macro alginate beads can be accomplished by various techniques including chemical, ionotropic, physical and mechanical methods. This work describes a method for preparing macro alginate beads through drop weight. The macro beads (2.85-3.85 mm) were prepared via different concentrations of alginate (0.5, 1.0, 1.5 and 2.0 g/L), dripping tip size (0.04-0.14 cm) and immersion into a predetermined concentration of calcium chloride (CaCl 2 ) bath. A custom made dripping vessel fabricated from acrylic plastic, connected to an adjustable dripping clamp was used to simulate the dripping process of the molten alginate at different tip sizes. It was observed that at different dripping tips, the correction factor for the alginate slurry was found in the range of 0.73-0.83. Meanwhile, the lost factor, K LF was observed at 0.93-2.3 and the shrinkage factors were limited to 2.00% from the overall distributed data. It was concluded that liquid properties had no effect on the liquid lost factor. The bead size prediction for different concentrations of alginate solution was compared to the experimental data. Subsequently, it was concluded that increasing the tip size caused the bead size to deviate almost 20% when compared to the experimental and predicted values, respectively.

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“…The soft alginate hydrogels produced in this study are designed as injectable carriers for the immunomodulatory cytokines and are not aimed at providing mechanical support to the infarcted area. The rheological properties of AC10 hydrogels are appropriate for intramyocardial injection via small needles (30G), and the viscoelastic behavior of AC10 hydrogels is comparable to that of other injectable biomaterials described in the literature (Oliveira et al, 2010;Munarin et al, 2014). Other acellular biomaterials have been injected in the myocardium with minimally invasive procedures for biomaterial-alone approaches or using biomaterials as carriers of growth factors, microribonucleic acids (miRNAs) or exosomes (Hernandez and Christman, 2017).…”

Section: Discussionmentioning

confidence: 99%

Engineering Immunomodulatory Biomaterials for Regenerating the Infarcted Myocardium

Bloise

Rountree

Polucha

et al. 2020

Front. Bioeng. Biotechnol.

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Coronary artery disease is a severe ischemic condition characterized by the reduction of blood flow in the arteries of the heart that results in the dysfunction and death of cardiac tissue. Despite research over several decades on how to reduce long-term complications and promote angiogenesis in the infarct, the medical field has yet to define effective treatments for inducing revascularization in the ischemic tissue. With this work, we have developed functional biomaterials for the controlled release of immunomodulatory cytokines to direct immune cell fate for controlling wound healing in the ischemic myocardium. The reparative effects of colony-stimulating factor (CSF-1), and anti-inflammatory interleukins 4/6/13 (IL4/6/13) have been evaluated in vitro and in a predictive in vivo model of ischemia (the skin flap model) to optimize a new immunomodulatory biomaterial that we use for treating infarcted rat hearts. Alginate hydrogels have been produced by internal gelation with calcium carbonate (CaCO 3) as carriers for the immunomodulatory cues, and their stability, degradation, rheological properties and release kinetics have been evaluated in vitro. CD14 positive human peripheral blood monocytes treated with the immunomodulatory biomaterials show polarization into pro-healing macrophage phenotypes. Unloaded and CSF-1/IL4 loaded alginate gel formulations have been implanted in skin flap ischemic wounds to test the safety and efficacy of the delivery system in vivo. Faster wound healing is observed with the new therapeutic treatment, compared to the wounds treated with the unloaded controls at day 14. The optimized therapy has been evaluated in a rat model of myocardial infarct (ischemia/reperfusion). Macrophage polarization toward healing phenotypes and global cardiac function measured with echocardiography and immunohistochemistry at 4 and 15 days demonstrate the therapeutic potential of the proposed immunomodulatory treatment in a clinically relevant infarct model.

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Characterization of Polymeric Solutions as Injectable Vehicles for Hydroxyapatite Microspheres

Cited by 18 publications

References 31 publications

Reactive hydroxyapatite fillers for pectin biocomposites

Reactive hydroxyapatite fillers for pectin biocomposites

Preparation and Characterization of Alginate Beads by Drop Weight

Engineering Immunomodulatory Biomaterials for Regenerating the Infarcted Myocardium

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