Characterization of Phosphate-Solubilizing Fluorescent Pseudomonads from the Rhizosphere of Seabuckthorn Growing in the Cold Deserts of Himalayas

Gulati, Arvind; Rahi, Praveen; Vyas, Pratibha

doi:10.1007/s00284-007-9042-3

Cited by 152 publications

(83 citation statements)

References 24 publications

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“…Another subgroup harboring many efficient phosphatesolubilizing bacteria was subgroup 1g (with reference strain SBW25). There are other studies that have identified Pseudomonas strains closely related to SBW25 as strong P solubilizers (4,13). For future studies, it would be interesting to test strong phosphate solubilizers selected from subgroups 1d and 1g for efficacy in planta in comparison to strains of other phylogenetic backgrounds.…”

Section: Discussionmentioning

confidence: 99%

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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Many root-colonizing pseudomonads are able to promote plant growth by increasing phosphate availability in soil through solubilization of poorly soluble rock phosphates. The major mechanism of phosphate solubilization by pseudomonads is the secretion of gluconic acid, which requires the enzyme glucose dehydrogenase and its cofactor pyrroloquinoline quinone (PQQ). The main aim of this study was to evaluate whether a PQQ biosynthetic gene is suitable to study the phylogeny of phosphate-solubilizing pseudomonads. To this end, two new primers, which specifically amplify the pqqC gene of the Pseudomonas genus, were designed. pqqC fragments were amplified and sequenced from a Pseudomonas strain collection and from a natural wheat rhizosphere population using cultivation-dependent and cultivation-independent approaches. Phylogenetic trees based on pqqC sequences were compared to trees obtained with the two concatenated housekeeping genes rpoD and gyrB. For both pqqC and rpoD-gyrB, similar main phylogenetic clusters were found. However, in the pqqC but not in the rpoD-gyrB tree, the group of fluorescent pseudomonads producing the antifungal compounds 2,4-diacetylphloroglucinol and pyoluteorin was located outside the Pseudomonas fluorescens group. pqqC sequences from isolated pseudomonads were differently distributed among the identified phylogenetic groups than pqqC sequences derived from the cultivation-independent approach. Comparing pqqC phylogeny and phosphate solubilization activity, we identified one phylogenetic group with high solubilization activity. In summary, we demonstrate that the gene pqqC is a novel molecular marker that can be used complementary to housekeeping genes for studying the diversity and evolution of plant-beneficial pseudomonads.

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Section: Discussionmentioning

confidence: 99%

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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“…The significance of this investigation is the reported phosphate solubilization and plant growth promotion by a psychrotolerant strain of Pseudomonas fragi CS11RH1. Earlier studies on phosphate solubilization/plant growth promotion by pseudomonads include P. fluorescens (Di Simine et al 1998), P. corrugata (Pandey & Palni 1998), P. aeruginosa (Musarrat et al 2000), P. stutzeri (Vazquez et al 2000), P. putida (Pandey et al 2008), P. rhizosphaerae , P. lutea (Peix et al 2004), P. trivialis and P. poae (Gulati et al 2008). P. fragi is commonly encountered in refrigerated dairy products and is distinguished from other pseudomonads by its inability to produce iron sequestering siderophores from the environment.…”

Section: Discussionmentioning

confidence: 99%

Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude Himalayan rhizosphere

et al. 2009

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Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas, are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the 16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30• C. Besides solubilizing P it produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant growth promoting ability of the bacterium.

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“…There have been a number of reports on plant growth promotion by bacteria (Kucey et al, 1989) that have the ability to solubilize inorganic or organic P from soil after their inoculation in soil. Wani et al (2007c) reported a synergistic effects of PSB and N 2 -fixing bacteria on chickpea plants with the significant increase of grain yield and uptake of P and N. Larger quantities of the insoluble P became soluble after the addition of PSB (Subba Rao, 1984) and concurrently use of PSB as inoculants enhances plant P uptake and crop yield (Rodriguez and Fraga, 1999;Gulati et al, 2007). Strains were able to produce organic acids such as oxalic acid, malic acid, succinic acid and propionic acid from both soil and roots of aerobic rice.…”

Section: Discussionmentioning

confidence: 99%

Contribution of phosphate-solubilizing bacteria in phosphorus bioavailability and growth enhancement of aerobic rice

Panhwar

Othman

Rahman

et al. 1970

Span. j. agric. res.

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The phosphate-solubilizing bacteria (PSB) can solubilize insoluble forms of phosphorous (P) into simple soluble forms that can be taken up by plants. The main focus of this study was to determine the effect of PSB on P availability in presence of different P rates for improved and sustainable rice (Oryza sativa L.) production under aerobic conditions. Triple superphosphate (TSP) at three levels (0, 30 and 60 kg ha -1 ) and two isolated PSB (Bacillus sp.) strains (PSB9 and PSB16) were tested in glasshouse conditions. Surface sterilized seeds of aerobic rice (M9 variety) were planted in plastic pots containing 3 kg of soil for 60 days. PSB strains exhibited capability of producing organic acids from soil and plant roots and increased yield of aerobic rice. Significantly, high P solubilization (28.7 mg kg -1 ) and plant uptake (7.94 mg kg -1 ) was found in PSB16 inoculated treatments at 30 kg ha -1 of P 2 O 5 . In this treatment were also observed high leaf chlorophyll content (34.57), photosynthesis rate (7.59 µmol CO 2 m -2 s -1 ) and root development. Isolated strains showed potential to make higher availability of P and increase content of organic acids from soil and roots at lower doses of TSP in aerobic rice. With the production of organic acids (oxalic, malic, succinic and propionic) higher amounts of P in the soil solution increased plant P uptake and resulted in higher plant biomass. The application of these potential inoculants in an appropriate combination with chemical fertilizers could be considered in organic and sustainable aerobic rice cultivation system. Additional key words: organic acids; solubilization; strains; triple superphosphate; uptake. ResumenContribución de las bacterias solubilizadoras de fosfato a la biodisponibilidad del fósforo y a la mejora del crecimiento del arroz aeróbico Las bacterias solubilizadoras de fosfato (PSB) son capaces de solubilizar formas insolubles de fósforo (P), para que puedan ser absorbidas por las plantas. Se estudió el potencial de dos cepas de PSB (Bacillus sp.), PSB9 y PSB16, para la solubilización del P y el aumento del crecimiento del arroz aeróbico (Oryza sativa L.) en condiciones de invernadero, utilizando superfosfato triple (TSP) a tres niveles (0, 30 y 60 kg ha -1 ). Se sembraron semillas de arroz aeró-bico (variedad M9) en macetas de plástico con 3 kg de suelo. Las cepas PSB fueron capaces de producir ácidos orgánicos a partir del suelo y de las raíces de las plantas y aumentaron el rendimiento del arroz aeróbico. Se encontró una alta y significativa solubilización de P (28,7 mg kg -1 ) y de absorción por la planta (7,94 mg kg -1 ) en los tratamientos inoculados con PSB16 utilizando 30 kg ha -1 de P 2 O 5 . En este tratamiento también se observó un alto contenido de clorofila (34,57), tasa de fotosíntesis (7,59 mmol CO 2 m -2 s -1 ) y desarrollo de raíces. Las cepas mostraron potencial para una mayor disponibilidad de P y aumento de ácidos orgánicos del suelo y las raíces a bajas dosis de TSP en el arroz aeróbico. Con la producción de ácidos ...

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Characterization of Phosphate-Solubilizing Fluorescent Pseudomonads from the Rhizosphere of Seabuckthorn Growing in the Cold Deserts of Himalayas

Cited by 152 publications

References 24 publications

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude Himalayan rhizosphere

Contribution of phosphate-solubilizing bacteria in phosphorus bioavailability and growth enhancement of aerobic rice

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