Characterization of pathogenic vibrios isolated from bivalve hatcheries in Galicia, NW Atlantic coast of Spain. Description of Vibrio tubiashii subsp. europaensis subsp. nov

Prado, Susana; Dubert, Javier; Barja, Juan L.

doi:10.1016/j.syapm.2014.11.005

Cited by 36 publications

(24 citation statements)

References 26 publications

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“…Initially, this taxon was described as a subespecies of V. tubiashii , V. tubiashii subsp. europaeus by Prado et al (2015). However, this study showed some taxonomic incongruities.…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spcontrasting

confidence: 48%

“…Later, studies carried out by Dubert et al (2016e) employing a polyphasic approach that included the WGS analysis, as well as phenotypic tests and chemotaxonomic techniques, supported their elevation to the rank of species. Strains described in the initial study were isolated from O. edulis and R. philippinarum during disease outbreaks in different Spanish hatcheries (Prado et al, 2005, 2015). Interestingly, Dubert et al (2016e) included in the description of the new taxa a French isolate initially identified as V. tubiashii , and highly pathogenic for C. gigas larvae and spat (Mersni-Achour et al, 2014, 2015; Travers et al, 2014).…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

“…Recently, Dubert et al (2017) have reported a mortality event in larvae of other clam species, namely R. decussatus , involving this bacterial species. In summary, V. europaeus is an emergent bivalve pathogen responsible of severe losses that affected Spanish and French hatcheries (Travers et al, 2014; Prado et al, 2015; Dubert et al, 2016e, 2017)…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

“…Interestingly, the vast majority virulence assays in larvae were carried out by immersion in Vibrio -inoculated seawater being a good reflect of the natural route of infection (Tubiash et al, 1965; Lodeiros et al, 1987; Gómez-León et al, 2005; Prado et al, 2005, 2015; Kesarcodi-Watson et al, 2009; Richards et al, 2014b; Rojas et al, 2015, 2016; Dubert et al, 2016d,e). Obviously, time-course of mortalities depends on the degree of virulence of the pathogenic strain and the bacterial concentration inoculated.…”

Section: Recent Advances On the Pathogenesis Of Vibriosismentioning

confidence: 99%

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New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

2017

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Hatcheries constitute nowadays the only viable solution to support the husbandry of bivalve molluscs due to the depletion and/or overexploitation of their natural beds. Hatchery activities include the broodstock conditioning and spawning, rearing larvae and spat, and the production of microalgae to feed all stages of the production cycle. However, outbreaks of disease continue to be the main bottleneck for successful larval and spat production, most of them caused by different representatives of the genus Vibrio. Therefore, attention must be paid on preventive and management measures that allow the control of such undesirable bacterial populations. The present review provides an updated picture of the recently characterized Vibrio species associated with disease of bivalve molluscs during early stages of development, including the controversial taxonomic affiliation of some of them and relevant advances in the knowledge of their virulence determinants. The problematic use of antibiotics, as well as its eco-friendly alternatives are also critically discussed.

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“…Initially, this taxon was described as a subespecies of V. tubiashii , V. tubiashii subsp. europaeus by Prado et al (2015). However, this study showed some taxonomic incongruities.…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spcontrasting

confidence: 48%

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

Section: Recent Advances On the Pathogenesis Of Vibriosismentioning

confidence: 99%

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New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

2017

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“…Our results show that the BA55 strain displayed DDH values below 70% with every other strain analyzed. This cut-off has been traditionally used for the delineation of species in prokaryotes, for both in silico 21 and experimental analysis 22 (Fig. 9A).…”

Section: Resultsmentioning

confidence: 99%

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

Restrepo

Bayot

Arciniegas

et al. 2018

Sci Rep

111

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Acute hepatopancreatic necrosis disease (AHPND) has extended rapidly, causing alarming shrimp mortalities. Initially, the only known causative agent was Vibrio parahaemolyticus carrying a plasmid coding for the mortal toxins PirVP. Recently, it has been found that the plasmid and hence the disease, could be transferred among members of the Harveyi clade. The current study performs a genomic characterization of an isolate capable of developing AHPND in shrimp. Mortality studies and molecular and histopathological analyses showed the infection capacity of the strain. Multilocus sequence analysis placed the bacteria as a member of the Orientalis clade, well known for containing commensal and even probiotic bacteria used in the shrimp industry. Further whole genome comparative analyses, including Vibrio species from the Orientalis clade, and phylogenomic metrics (TETRA, ANI and DDH) showed that the isolate belongs to a previously unidentified species, now named Vibrio punensis sp. nov. strain BA55. Our findings show that the gene transfer capacity of Vibrio species goes beyond the clade classification, demonstrating a new pathogenic capacity to a previously known commensal clade. The presence of these genes in a different Vibrio clade may contribute to the knowledge of the Vibrio pathogenesis and has major implications for the spread of emerging diseases.

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Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment

et al. 2015

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The characterization of antibiotic-resistant vibrios isolated from shellfish aquaculture is necessary to elucidate the potential transfer of resistance and to establish effective strategies against vibriosis. With this aim, we analyzed a collection of bacterial isolates obtained from 15 failed hatchery larval cultures that, for the most part, had been treated experimentally with chloramphenicol to prevent vibriosis. Isolates were obtained during a 2-year study from experimental cultures of five different clam species. Among a total of 121 Vibrio isolates studied, 28 were found to be chloramphenicol resistant, suggesting that the shellfish hatchery had been using a sublethal concentration of the antibiotic. Interestingly, chloramphenicol-resistant vibrios showed also resistance to tetracycline and amoxicillin (group A; n = 19) or to streptomycin (group B; n = 9). Chloramphenicol-resistant vibrios were subjected to a PCR amplification and DNA sequencing of the chloramphenicol acetyltransferase genes (cat), and the same approach was followed to study the tetracycline resistance markers (tet). 16S ribosomal RNA (rRNA) gene sequencing revealed that chloramphenicol-resistant vibrios pertained mostly to the Splendidus clade. Conjugation assays demonstrated that various R-plasmids which harbored the cat II/tet(D) genes and cat III gene in groups A and B respectively, were transferred to E. coli and bivalve pathogenic vibrios. Most interestingly, transconjugants exhibited the antibiotic resistance patterns of the donors, despite having been selected only on the basis of chloramphenicol resistance. This is the first report carried out in a bivalve hatchery elucidating the persistence of resistant vibrios, the mechanisms of antibiotic resistance, and the transfer of different R-plasmids.

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Characterization of pathogenic vibrios isolated from bivalve hatcheries in Galicia, NW Atlantic coast of Spain. Description of Vibrio tubiashii subsp. europaensis subsp. nov

Cited by 36 publications

References 26 publications

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment

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