Characterization of NDM-5-producing <i>Enterobacteriaceae</i> isolates from retail grass carp (<i>Ctenopharyngodon idella</i>) and evidence of <i>bla</i>_NDM-5-bearing IncHI2 plasmid transfer between ducks and fish

Abstract

We aimed to characterize NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou, China. A total of 196 intestinal samples of grass carp collected in 2019 were screened for carbapenemase genes. Characterization of bla NDM-5 positive isolates and plasmids was determined by antimicrobial susceptibility testing, conjugation experiments, Illumina HiSeq, and Nanopore sequencing. One Citrobacter freundii and six Escherichia coli strains recovered from seven intestinal samples were verified as bla NDM-5 carriers (3.57%, 7/196). The bla NDM-5 genes were located on the IncX3 ( n =5), IncHI2 ( n =1), or IncHI2-IncF ( n =1) plasmids. All bla NDM-5 -bearing plasmids were transferred by conjugation at frequencies of ~10 −4 –10 −6 . Based on sequence analysis, the IncHI2 plasmid pHNBYF33-1 was similar to other bla NDM-5 -carrying IncHI2 plasmids deposited in GenBank from Guangdong ducks. In all IncHI2 plasmids, bla NDM-5 was embedded in a novel transposon, Tn 7051 (IS 3000 -ΔIS Aba125 -IS 5 -ΔIS Aba125 - bla NDM-5 - ble MBL - trpF - tat -∆ dct -IS 26 -∆ umuD -∆IS Kox3 -IS 3000 ), which was identical to the genetic structure surrounding bla NDM-5 found in some IncX3 plasmids. The IncHI2-IncF hybrid plasmid pHNTH9F11-1 was formed by homologous recombination of the bla NDM-5 -carrying IncHI2 plasmid and a heavy-metal-resistant IncF plasmid through ∆Tn 1721 . To the best of our knowledge, this is the first report on the characterization of bla NDM-5 -bearing plasmids in fish in China. The IncHI2 plasmid pHNBYF33-1 may be transmitted from ducks, considering the common duck-fish freshwater aquaculture system in Guangdong. Tn 7051 is likely responsible for the transfer of bla NDM-5 from IncX3 to IncHI2 plasmids in Enterobacteriaceae , resulting in the expansion of transmission vectors of bla NDM-5 .

Molecular Epidemiology of New Delhi Metallo-β-Lactamase-Producing Escherichia coli in Food-Producing Animals in China

“…Since its first report in British, bla NDM-5 has been detected in clinical isolates from Europe, Asia, South America, the United States of America and Australia (Nakano et al, 2014;Wailan et al, 2015;Baek et al, 2019;López-Hernández et al, 2020;Ramadan et al, 2020;Costa et al, 2021). Notably, the prevalence of bla NDM-5 isolates from food-producing animals has been sporadic in aboard (Ghatak et al, 2013), although there are more relevant reports in China (Yang et al, 2016;Kong et al, 2017a;Yao et al, 2020;Zhao et al, 2021;Lv et al, 2022). In the current study, our results revealed a wide contamination of NDM-positive Enterobacteriaceae in ten different provinces.…”

Clonal, Plasmidic and Genetic Diversity of Multi-Drug-Resistant Enterobacterales from Hospitalized Patients in Tripoli, Libya

“…The bla NDM-5 and bla CTX-M-15 genes were co-carried on an additional large-size IncFIB/IncHI2 plasmid (360,100 bp) also displaying the armA , aph(3′)-VI , aac(6′)-Ib , aadA1 , qnrS1 , sul1 , sul2 and dfrA5 genes. Such hybrid plasmids have already been reported to carry different resistance genes such as mcr-1 or bla NDM-5 [ 19 , 20 ].…”

F18:A-:B1 Plasmids Carrying blaCTX-M-55 Are Prevalent among Escherichia coli Isolated from Duck–Fish Polyculture Farms

“…Duck–fish polyculture is a common circular farming model in the Pearl River Delta in southern China, specifically in Guangdong Province. In this model, duck manure is discharged directly without treatment, and a large number of ARGs or residual agents can directly contaminate fish ponds, promoting the transmission of ARGs between ducks and fish [ 14 , 23 , 24 ]. Previous studies have shown that fish–duck integrated farming systems have become a hotspot environment for the occurrence and proliferation of ARGs and ARB, and both ARGs and pathogen-related ARB have been detected in the water and sediment of this culture system [ 12 , 13 , 14 ].…”