Characterization of Nanoparticles for Therapeutics

Hall, Jennifer B.; Dobrovolskaia, Marina A.; Patri, Anil K.; McNeil, Scott E.

doi:10.2217/17435889.2.6.789

Cited by 348 publications

(264 citation statements)

References 67 publications

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“…From the TEM images, an average particle diameter and standard deviation of 17.8 and 1.7 nm, respectively, were recorded for the citrate-capped AuNPs, compared to 17.9 and 1.9 nm, respectively, for the AuNPs functionalized with 16.8 μg/ml of PEG. It was expected that no significant difference in size between citrate-and PEG-capped AuNP would be observed via TEM, as only the gold core is visible at the acceleration voltage used [24].…”

Section: Resultsmentioning

confidence: 99%

“…The biological response can be altered using capping agents such as polyethylene glycol (PEG) also referred to as polyethylene oxide (PEO) [20,21], mercaptosuccinic acid, various proteins [21] or other biomolecules [2]. Such functionalization has led to an extensive research effort investigating AuNPs as carriers for a range of biomolecules and drugs [22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

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Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media

et al. 2011

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Thiol-terminated polyethylene glycol (PEG) is commonly used to functionalize the surface of gold nanoparticles (AuNPs) in order to improve their in vivo stability and to avoid uptake by the reticular endothelial system. Although it has been reported that AuNPs functionalized with tethered PEG are stable in biological media, the influence of chain density remains unclear. This study investigates the influence of PEG capping density on the stability of washed and dried AuNPs in: water, phosphate-buffered saline solution (PBS), phosphate-buffered saline solution containing bovine serum albumin (PBS/BSA), and dichloromethane (DCM). PEG coating had a dramatic effect on stability enabling stable suspensions to be produced in all the media studied. A linear relationship was observed between capping density and stability in water and DCM with a somewhat lower stability observed in PBS and PBS/BSA. A maximum PEG loading level of ∼14 wt.% was achieved, equivalent to a PEG surface density of ∼1.13 chains/nm 2 .

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media

et al. 2011

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“…Due to the defective vascular architecture and poor lymphatic drainage, nanoparticles can extravasate through these gaps into extravascular spaces and accumulate inside tumor tissues (26,27). This phenomenon is known as the enhanced permeability and retention (EPR) effect (28,30), which has been generally observed in many types of solid tumors. Since the prepared MPEG-PCL is small enough (~20 nm), it can increase the selectivity of the available cytotoxic agents by delivering them specifically to tumor tissue, which allows maximal accumulation and the deepest penetration into tumors.…”

Section: Discussionmentioning

confidence: 99%

Antitumor activity of monomethoxy poly(ethylene glycol)-poly (ε-caprolactone) micelle-encapsulated doxorubicin against mouse melanoma

Qian¹

2011

Oncol Rep

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Abstract. Doxorubicin (Dox) is one of the most commonly used and highly effective antineoplastic agents, but the clinical application of this broad spectrum drug is largely hampered by its poor stability and serious toxicity to normal tissues. Hence, it is essential to improve the therapeutic effect and decrease the systematic toxicity for the administration of doxorubicin. In our study, doxorubicin was incorporated into monomethoxy poly(ethylene glycol)-poly(Â-caprolactone) (MPEG-PCL) micelle by a self-assembly method. The cytotoxicity and cellular uptake efficiency of Dox-loaded MPEG-PCL (Dox/MPEG-PCL) micelle against B16-F10 murine melanoma cells was examined by the methylthiazoltetrazolium (MTT) test and flow cytometry. The antitumor activity of Dox/MPEG-PCL was evaluated in C57BL/6 mice injected subcutaneously with B16-F10 cells. Toxicity was evaluated in tumor-free mice. Meanwhile, tumor proliferation, intratumoal angiogenesis and apoptotic cells were evaluated by PCNA, CD31 staining and TUNEL assay, respectively. Encapsulation of doxorubicin in MPEG-PCL micelle improved the cytotoxicity of doxorubicin and enhanced its cellular uptake on B16-F10 cell in vitro. Administration of Dox/MPEG-PCL micelle resulted in significant inhibition (75% maximum inhibition relative to controls) in the growth of B16-F10 tumor xenografts and prolonged the survival of the treated mice (P<0.05). These anti-tumor responses were associated with marked increase of tumor apoptosis and notable reduction of cell proliferation and intratumoral microvessel density (P<0.05). The system toxicity also decreased in the Dox/MPEG-PCL group compared with free doxorubicin group. Our data indicate that the encapsulation of doxorubicin in MPEG-PCL micelle improved the anti-tumor activity in vivo without conspicuous systemic toxic effects.

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“…Recent findings have also shown that incubating NPs with human plasma, from subjects with different diseases and medical conditions, results in protein coronas of different composition [32]. Since blood composition is highly complex, the analysis of the NP behavior in complete (human) blood is very challenging and the studies are usually done in vitro using defined suspension conditions and/or in vivo experiments (for a review see Hall et al [33]). Indeed, most research is focused on the identification of surface adsorbed proteins on NPs with respect to their physicochemical properties [34].…”

Section: Bloodmentioning

confidence: 99%