Characterization of monoclonal antibodies against human chorionic somatomammotropin: Competitive screening and determination of the affinity constants

Mariani, Massimo A.; Neri, Paolo; Cianfriglia, Maurizio; Armellini, D.; Barbarulli, G.; Antoni, G.

doi:10.1016/0022-1759(84)90204-7

Cited by 20 publications

(2 citation statements)

References 14 publications

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“…The significative difference in the value of K, for the MAb toward CEM-VBL100 and CEM-VBL50 cell lines, mainly reflects a different number of P-glycoprotein-bound-molecules more than an alteration in the binding affinity. Unfortunately, no reliable values of the number of binding sites per cell may be calculated by an indirect immunoreaction (Mariani et al, 1984).…”

Section: Afinity Of Mab57 For Its Epitopementioning

confidence: 99%

Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P‐glycoprotein): Determination of p‐glycoprotein expression in multi‐drug‐resistant kb and cem cell variants

Cenciarelli

Currier

Willingham

et al. 1991

Intl Journal of Cancer

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We isolated an IgG2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDRI gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDRI gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDRI gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDRI gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.

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Section: Afinity Of Mab57 For Its Epitopementioning

confidence: 99%

Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P‐glycoprotein): Determination of p‐glycoprotein expression in multi‐drug‐resistant kb and cem cell variants

Cenciarelli

Currier

Willingham

et al. 1991

Intl Journal of Cancer

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“…The data are analyzed by linear transform by either the Scatchard (13) or the Sips (14) equations to produce a plotted line with a specific degree of slope. Several investigators have reported that these calculations are cumbersome and inaccurate and tend to fail to linearize the curves (2,4,6,16).…”

mentioning

confidence: 99%

Calculation of antibody affinity in homogeneous and heterogeneous systems

Chalquest

1988

J Clin Microbiol

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An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes

et al. 1991

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An epitope of human chorionic somatomammotropin for one of the monoclonal antibodies raised against the whole antigen has been identified. We compared the release of peptides from limited proteolysis of the antigen in the presence and absence of the related antibody. Using enzymes of different specificity, we could determine the amino acid sequence that can be considered at least inclusive of the epitope. The monoclonal antibody selected is 100% cross-reactive with human growth hormone, so the antigenic determinant identified is shared by the two protein hormones.

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Characterization of monoclonal antibodies against human chorionic somatomammotropin: Competitive screening and determination of the affinity constants

Cited by 20 publications

References 14 publications

Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P‐glycoprotein): Determination of p‐glycoprotein expression in multi‐drug‐resistant kb and cem cell variants

Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P‐glycoprotein): Determination of p‐glycoprotein expression in multi‐drug‐resistant kb and cem cell variants

Calculation of antibody affinity in homogeneous and heterogeneous systems

An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes

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