Characterization of metabolic detoxifying enzymes in an insecticide resistant strain of &lt;i&gt;Aedes aegypti&lt;/i&gt; harboring homozygous S989P and V1016G &lt;i&gt;kdr&lt;/i&gt; mutations

Choovattanapakorn, Nontawan; Yanola, Jintana; Lumjuan, Nongkran; Saingamsook, Jassada; Somboon, Pradya

doi:10.7601/mez.68.19

Cited by 12 publications

(10 citation statements)

References 42 publications

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“…aegypti larvae prior to treatment with P. crispum oil demonstrated significantly higher levels of some detoxifying enzymes, such as GSTs, α-/β-ESTs, ACP and MFO in resistant PMD-R and/or UPK-R, than those in susceptible MCM-S. These findings were in agreement with those of many biochemical studies, which evaluated various pest species in either laboratory or field populations, and demonstrated increased levels of detoxifying enzymes such as GSTs [48,49], α-/β-ESTs [29,[50][51][52][53], ACP [54,55], and MFO [56][57][58] [15,59], and elevation of enzyme activity implicate an enhancer in the tolerance of mosquitoes to insecticides [50,51,60,61]. In consequence, resistance to some insecticides has been linked to an increment in insecticide metabolism through the up-regulation or structural changes of detoxifying enzymes [62,63].…”

Section: Resultssupporting

confidence: 84%

Biochemical Effects of Petroselinum crispum (Umbellifereae) Fruit Oil on the Pyrethroid Susceptible and Resistant Strains of Aedes aegypti (Diptera: Culicidae)

Intirach¹,

Junkum²,

Lumjuan³

et al. 2018

Preprint

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As part of the ongoing screening research for local edible plants in Thailand, Petroselinum crispum fruit oil was considered as a potential bioinsecticide with proven antimosquito activity against both the pyrethroid susceptible and resistant strains of Aedes aegypti. Due to the comparative mosquitocidal efficacy on these mosquitoes, this plant oil is promoted as a natural alternative and attractive candidate for further study in monitoring resistance of mosquito vectors. Therefore, the aim of this study was to evaluate the impact of P. crispum oil on the biochemical characteristics of the target mosquito larvae of Ae. aegypti, by determining quantitative changes of key enzymes responsible for xenobiotic detoxification, including glutathione-S-transferases (GSTs), α- and β-esterases (α-/β-ESTs), acetylcholinesterase (AChE), acid and alkaline phosphatases (ACP and ALP) and mixed-function oxidases (MFO). Three populations of Ae. aegypti, comprising the pyrethroid susceptible Muang Chiang Mai-susceptible (MCM-S) strain and the pyrethroid resistant Pang Mai Dang-resistant (PMD-R) and Upakut-resistant (UPK-R) strains, were used as test organisms. Biochemical study of Ae. aegypti larvae prior to treatment with P. crispum oil revealed that apart from AChE, the baseline activity of most defensive enzymes, such as GSTs, α-/β-ESTs, ACP, ALP and MFO, in resistant UPK-R or PMD-R, was higher than that determined in susceptible MCM-S. However, after 24-h exposure to P. crispum oil, the pyrethroid susceptible and resistant Ae. aegypti showed similarity in biochemical features, with alterations of enzyme activity in the treated larvae, as compared to the controls. A significant increase in the activity levels of GSTs, α-/β-ESTs, ACP and ALP was recorded in all strains of P. crispum oil-treated Ae. aegypti larvae, whereas MFO and AChE activity in these mosquitoes was decreased. The recognizable larvicidal capability on pyrethroid resistant Ae. aegypti, and the inhibitory effect on AChE and MFO, emphasized the potential of P. crispum oil as an attractive alternative application for management of mosquito resistance in current and future control programs.

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Section: Resultssupporting

confidence: 84%

Biochemical Effects of Petroselinum crispum (Umbellifereae) Fruit Oil on the Pyrethroid Susceptible and Resistant Strains of Aedes aegypti (Diptera: Culicidae)

Intirach¹,

Junkum²,

Lumjuan³

et al. 2018

Preprint

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“…Although temephos resistance has been linked to increased levels of esterases, the specific esterase and inhibitors have not been identified [49]. Increased MFO activity has been suggested to play a partial role in pyrethroid resistance as well with kdr acting as the major mechanism [50].…”

Section: Discussionmentioning

confidence: 99%

Resistance to commonly used insecticides and underlying mechanisms of resistance in Aedes aegypti (L.) from Sri Lanka

et al. 2020

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Background: Drastic increases of dengue fever (DF) over the past few years have prompted studies on the development of resistance to insecticides in the mosquito vector, Aedes aegypti (Linnaeus). In Sri Lanka control of the vector population is essentially achieved using larvicides (temephos) and adulticides (principally pyrethroids). The present study investigates resistance to commonly used insecticides and underlying mechanisms of Ae. aegypti in selected sites in Sri Lanka. Methods: In this study, susceptibility to three commonly used adulticides (malathion, permethrin and deltamethrin) and the larvicide temephos were tested for Ae. aegypti sampled from five localities in Sri Lanka using WHO dose diagnostics tests. In addition, we performed dose-response tests for permethrin to determine lethal concentrations (LCs) with CDC bottle bioassays. An assessment of the activity of metabolic detoxifying enzymes (multifunction oxidases (MFOs), glutathione S-transferases (GSTs) and esterases) and determination of frequency of the kdr mutations (F1534C, V1016G and S989P) were also carried out to ascertain the associated resistance mechanisms. Kdr genotype frequencies were compared with samples collected from the same sites in 2015 to determine the change of allele frequencies over the years. Results: The present study revealed resistance in all Ae. aegypti populations studied, with low mortality percentages for both permethrin (10-89%) and deltamethrin (40-92%). Dose response tests revealed highest resistance ratios (RR) for permethrin and temephos from Colombo district whereas Puttalum district exhibited the lowest. High frequencies of the 1534C allele (0.052-0.802) were found in the study sites in 2017. Comparison with samples collected in 2015 revealed a substantial increase in this allele. The activity of MFOs and p-nitro phenyl-acetate esterase was significantly greater in most Sri Lankan populations in comparison to that of the New Orleans (NO) susceptible strain. In contrast, the activity of α-esterase and β-esterase was similar or lower than that in the NO strain.

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“…aegypti [ 114 ]. The latest study demonstrated that the aforementioned enzymes were either unrelated or merely contributed partially in pyrethroid resistance [ 115 ]. In regards to the discrepancies of these enzymes as contributory factors to pyrethroid resistance in Ae.…”

Section: Present Situation Of Pyrethroid Susceptibility In mentioning

confidence: 99%

Pyrethroid resistance in the dengue vector Aedes aegypti in Southeast Asia: present situation and prospects for management

et al. 2018

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Human arboviral diseases transmitted by Aedes aegypti such as dengue, Zika, chikungunya and yellow fever remain global public health threats to date. Of these diseases, dengue fever is particularly prevalent in Southeast Asia. Relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control Ae. aegypti, especially in the course of disease outbreaks. Here, we compile the largest contemporary database for susceptibility profiles and underlying mechanisms involved in Ae. aegypti resistant to pyrethroids in Southeast Asia. The extensive use of pyrethroids inevitably elicit different levels of resistance to numerous populations despite the presence of geographical isolation. The most common mechanisms of pyrethroid resistance that have been identified in Ae. aegypti includes mutations in the voltage sensitive sodium channel gene (Vssc gene) and metabolic-mediated insecticide resistance. Aedes aegypti develops resistance to pyrethroids by acquisition of one or several amino acid substitution(s) in this Vssc gene. Enzymes involved in metabolic-mediated detoxification (i.e. monooxygenases, glutathione-S-transferases and esterases) have been reported to be related to pyrethroid resistance but many specific contributory enzymes are not completely studied. An inadequate amount of data from some countries indicates an urgent need for further study to fill the knowledge gaps. Perspectives and future research needs are also discussed.

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Characterization of metabolic detoxifying enzymes in an insecticide resistant strain of <i>Aedes aegypti</i> harboring homozygous S989P and V1016G <i>kdr</i> mutations

Cited by 12 publications

References 42 publications

Biochemical Effects of <em>Petroselinum crispum</em> (Umbellifereae) Fruit Oil on the Pyrethroid Susceptible and Resistant Strains of <em>Aedes aegypti</em> (Diptera: Culicidae)

Biochemical Effects of <em>Petroselinum crispum</em> (Umbellifereae) Fruit Oil on the Pyrethroid Susceptible and Resistant Strains of <em>Aedes aegypti</em> (Diptera: Culicidae)

Resistance to commonly used insecticides and underlying mechanisms of resistance in Aedes aegypti (L.) from Sri Lanka

Pyrethroid resistance in the dengue vector Aedes aegypti in Southeast Asia: present situation and prospects for management

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