Characterization of mesenchymal stem cells from human vocal fold fibroblasts

Abstract

Objective/Hypothesis Mesenchymal stem cells (MSCs) originally isolated from bone marrow, are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue. Study Design In vitro study Methods HVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors, based on their attachment to culture dishes and their morphology, and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs as well as the ability to differentiate into cells of mesenchymal lineage, i.e. fat, bone and cartilage. We investigated the immunophenotype of these cells before and after interferon-γ (INF- γ) stimulation. Results HVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of HLA and co-stimulatory molecules, after stimulation with INF- γ compared to MSCs derived from BM and AT. Conclusions Based on our findings hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose VF fibroblasts are MSCs resident in the vocal fold lamina propria.

Dynamic Vibration Cooperates with Connective Tissue Growth Factor to Modulate Stem Cell Behaviors

“…6,7 MSCs are a suitable alternative to primary vocal fold fibroblasts (VFFs) for vocal fold tissue engineering, considering their similar phenotype, multipotency, and immunoregulatory functions. 6,8 Mounting evidence confirms the regulatory role of the tissue-specific microenvironment in controlling the behaviors of MSCs.…”

The Effect of Mesenchymal Stromal Cell–Hyaluronic Acid Hydrogel Constructs on Immunophenotype of Macrophages

“…7,23 BM-and AT-MSCs were cultured in a 37°C incubator with 5% CO 2 -95% humidified atmosphere with standard media (alpha minimum essential media supplemented with 10% fetal bovine serum [FBS-Hyclone, Logan, UT], 1 · nonessential amino acids [NEAA, Sigma Aldrich], and 4 mM Lglutamine [Sigma Inc.], 100 U/mL penicillin, and 0.01 mg/ mL streptomycin sulfate [Sigma Inc.]). VF-MSCs were cultured in Dulbecco's modified essential medium with 10% FBS, 100 U/mL penicillin, 0.01 mg/mL streptomycin sulfate, and 1 · NEAA (all from Sigma Inc.).…”

“…[3][4][5] These progenitor cells isolated from different tissue sources all have the capability to differentiate into multiple cell lineages (such as bone, fat, and cartilage), and have specific cell surface marker characteristics, [6][7][8] though there is evidence to suggest that these cells can have phenotypical differences based on their tissue source of origin. 9 Most recently, unique immunomodulatory properties of MSCs, such as their potential use across HLA barriers, have been an area of active investigation as such properties make them of much more value in regenerative medicine.…”

Modulating the Behaviors of Mesenchymal Stem Cells Via the Combination of High-Frequency Vibratory Stimulations and Fibrous Scaffolds

“…11 Moreover, studies have shown that primary vocal fold fibroblasts (PVFFs) and MSCs share similar cell surface markers, immunophenotypic characteristics, and differentiation potential. 12 Thus, MSCs are a suitable alternative to PVFFs for vocal fold tissue engineering. MSCs are naturally sensitive to their environment, and each tissue contains a unique stem cell niche that fosters the tissuespecific stem cell differentiation and tissue development.…”