Characterization of ladybird Henosepilachna vigintioctopunctata transcriptomes across various life stages

Zhang, Qi-Lin; Wang, Zewei; Guo, Jun; Deng, Xian-Yu; Chen, Jun-Yuan; Lü, Lin

doi:10.1038/sdata.2018.93

Cited by 30 publications

(52 citation statements)

References 25 publications

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“…Moreover, we found that the five white transcripts were detectable from embryo (egg) to adults. In agreement with our result, the five white transcripts were discovered in the transcriptomes from egg, larva, pupa and adult (Zhang et al, 2018). Furthermore, white1, white3, white4 and white5 were highly expressed in the eggs and adults, whereas white2 was abundantly transcribed at the early stage within each instar.…”

Section: Discussionsupporting

confidence: 91%

“…Overall, five genes encoding ABCG transporters were identified. The five transcripts presented in each of the transcriptomes were obtained from egg, larva, pupa and adult (Zhang et al, 2018) (Table S1).…”

Section: Identification Of White Genesmentioning

confidence: 99%

“…Henosepilachna vigintioctopunctata (Fabricius) (Coleoptera: Coccinellidae) is one of the most serious insect pests in Asian countries (Zhang et al, 2018). The beetle colonizes a large number of nightshades, such as potato, eggplant, tomato, pepper and tobacco, and cucurbits such as cucumber, white gourd and loofah.…”

Section: Introductionmentioning

confidence: 99%

“…The destructive potential of H. vigintioctopunctata is high at both the larval and adult stages, leading to around 60% loss of crop production (Kawazu, 2014;Sharma et al, 2012). Molecular resources for H. vigintioctopunctata have only recently been developed (Guo et al, 2019;Zhang et al, 2018). Moreover, it has been documented that dsRNA can successfully knock down target genes in H. vigintioctopunctata (Hashiro et al, 2019;Ohde et al, 2009;Wu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Functional divergence of white genes in Henosepilachna vigintioctopunctata revealed by RNA interference

Kang

et al. 2020

Insect Molecular Biology

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Henosepilachna vigintioctopunctata is a serious pest of Solanaceae and Cucurbitaceae in many Asian countries. RNA interference (RNAi) can effectively reduce transcript abundance in this beetle, offering opportunities to explore the biological function of specific genes. The white gene encodes a half‐type ATP‐binding cassette transporter that plays an essential role in tryptophan, guanine and uric acid transport across membranes. Mutations that disrupt the function of white are known to cause eye pigmentation phenotypes in many insect species. Here, we found evidence for five white gene paralogues present in H. vigintioctopunctata transcriptome datasets sequenced from a range of developmental stages. We individually knocked down each of the five white genes through the injection of corresponding double‐stranded RNAs (dsRNAs) to the fourth‐instar larvae to determine whether functional divergence has occurred. We found that injecting 1 μg dswhite3 caused compound eye colour of pupae and adults to develop as red/brown and brown, respectively, compared with black eyes in control beetles. Injection of 2 μg dswhite3 increased RNAi efficacy and produced a clearer eye colour phenotype. At both doses, the ocular diaphragm (a ring of black pigment surrounding each eye) did not change in the white3 RNAi hypomorphs. Moreover, our data revealed that injection of dswhite2 at the fourth‐instar larval stage impaired the climbing ability of both male and female adults. Our results confirmed, for the first time, functional divergence of duplicated white genes in an insect species.

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Section: Discussionsupporting

confidence: 91%

Section: Identification Of White Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional divergence of white genes in Henosepilachna vigintioctopunctata revealed by RNA interference

Kang

et al. 2020

Insect Molecular Biology

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“…Transcriptome sequencing is an efficient and low-cost method by which to explore gene expression patterns at multiple developmental stages 6,7 , tissues in insects 8,9 . Many insect transcriptomes have been obtained using next-generation sequencing techniques for functional genomic studies 10,11 .…”

Section: Background and Summarymentioning

confidence: 99%

Genome-wide gene expression profiling of the melon fly, Zeugodacus cucurbitae, during thirteen life stages

Wei

Dong

et al. 2020

Sci Data

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The melon fly, Zeugodacus cucurbitae (Coquillett), is an important destructive pest worldwide. Functional studies of the genes associated with development and reproduction during different life stages are limited in Z. cucurbitae. There have yet to be comprehensive transcriptomic resources for genetic and functional genomic studies to identify the molecular mechanisms related to its development and reproduction. In this study, we comprehensively sequenced the transcriptomes of four different developmental stages: egg, larva, pupa, and adults. Using the Illumina RNA-Seq technology, we constructed 52 libraries from 13 stages with four biological replicates in each and generated 435.61 Gb clean reads. We comprehensively characterized the transcriptomes with highcoverage mapping to the reference genome. A total of 13,760 genes were mapped to the reference genome, and another 4481 genes were characterized as new genes. Finally, 14,931 genes (81.85%) were functionally annotated against six annotation databases. This study provides the first comprehensive transcriptome data of all developmental stages of Z. cucurbitae, and will serve as a valuable resource for future genetic and functional studies. These results indicated that sequencing quality was high. Clean reads were separately aligned to the reference genome using HISAT2 33 .Transcriptome quality assessment. The saturation of sequencing was also analyzed using RSeQC 2.3.6 package, and the data showed a high saturation of each sample sequencing (Fig. 3a). Most of the reads were mapped to the coding sequence region (Fig. 3b). The alignment of the superreads to the reference genome resulted in a total of 41,530 transcripts, and 34.03% were longer than 2700 bp, and 75.96% were longer than 900 bp (Fig. 3c). Analyses of the structures of the transcripts suggested that 11,506 transcripts were potential novel transcript isoforms (Fig. 3d), and 22,068 transcripts were completely matched to the exons.

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Yellow‐b, ‐c, ‐e and ‐h are required for normal body coloration in Henosepilachna vigintioctopunctata

Wang

Jin

et al. 2021

Arch Insect Biochem Physiol

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The involvement of yellow genes y-b, y-c, y-e, and y-h in cuticle tanning has poorly been clarified. In the present paper, six putative yellow (y-y, y-b, y-c, y-e y-f, and y-h) genes were identified in Henosepilachna vigintioctopunctata.Hvy-b, Hvy-c, Hvy-e, and Hvy-h were abundantly transcribed at early larval and late pupal stages, especially in the epidermis. Accordingly, RNA interference (RNAi) experiments were performed by an injection of dsy-b, dsy-c, dsye, or dsy-h into the second instar larvae and 1-day-old pupae. The head capsule, scoli and strumae, and legs in the fourth-instar larvae became blacker; the blackish spots in the pupae were darkened and widened after RNAi of Hvy-b, compared with those of dsegfp-treated controls.Depletion of Hvy-b at the 1-day-old pupal stage expanded two pair of black markings on the sternum of the metathorax, and darkened the black patched on the sterna of the abdomen segments I-VI in the resultant adults.Depletion of Hvy-e caused darker pigmented adult body and elytral cuticles than those of dsegfp-introduced controls. However, there was no obvious difference in pigmentation of the black markings. Hvy-h-deficient larvae displayed dark yellow body color, whereas the body color of the dsegfp-injected control was pale yellow. There was no obvious difference in coloration of larval specific-black markings or pupal cuticle between dsHvy-h-and dsegfptreated animals. Moreover, silence of Hvy-c at the second

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Characterization of ladybird Henosepilachna vigintioctopunctata transcriptomes across various life stages

Cited by 30 publications

References 25 publications

Functional divergence of white genes in Henosepilachna vigintioctopunctata revealed by RNA interference

Functional divergence of white genes in Henosepilachna vigintioctopunctata revealed by RNA interference

Genome-wide gene expression profiling of the melon fly, Zeugodacus cucurbitae, during thirteen life stages

Yellow‐b, ‐c, ‐e and ‐h are required for normal body coloration in Henosepilachna vigintioctopunctata

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