Characterization of Soybean yellow shoot virus, a New Member of the Family Potyviridae Infecting Soybean Plants in Brazil

Abstract

A new virus species, belonging to the family Potyviridae and capable of infecting most of the soybean cultivars grown in Brazil, was collected in Lavras, Minas Gerais, Brazil, and named Soybean yellow shoot virus (SoyYSV). In this study, the complete 9,052-nucleotide genome of SoyYSV was determined and the structural, biological, and molecular properties of the virus were investigated. The SoyYSV genome encoded a single polyprotein that could be subsequently cleaved, generating 11 proteins. The SoyYSV genome shared 49% nucleotide and 36% amino acid sequence identity with Blackberry virus Y. However, the P1 protein of SoyYSV was much smaller and lacked the ALK1 domain characteristic of the genus Brambyvirus. Electron microscopy revealed flexuous filamentous virus particles, 760 to 780 nm in length, and cytoplasmic inclusions typical of those found in plant cells infected with Potyviridae species. In addition to soybean, SoyYSV infected species in the Amaranthaceae, Caricaceae, Fabaceae, and Solanaceae families. Among the most common potyviruses present in Brazil, only SoyYSV induced local necrotic lesions in Carica papaya L. SoyYSV was transmissible by Myzus persicae and Aphis gossypii but lacked the HC-Pro domain required for aphid transmission in other potyviruses. No seed transmission in soybean was observed.

Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity

“…Even with the absence of aphid transmission domains in the PeSV proteins, the possibility of aphid transmission should not be overlooked due to the high population of aphids observed on pomegranate trees in early spring and morphologically identified as Aphis punicae (Passerini) (Çaglayan, unpublished data). In fact, a new soybean virus, named soybean yellow shoot virus (SoyYSV) belonging to the Potyviridae, was recently shown to be transmitted by Myzus persicae and Aphis gossypii while lacking the HC-Pro domain required for aphid transmission found in other potyviruses [29].…”

Molecular and biological characterization of an isolate of passiflora virus Y (Potyvirus) naturally infecting soybean (Glycine max) in Brazil

“…Several diseases affect soybean production and viruses cause the major problems, with at least 46 virus species identi ed infecting soybean worldwide [2]. About 20 virus species are described in Brazil, which belongs to the genera Alfamovirus (1 species), Begomovirus (5), Bromovirus (1), Carlavirus (1), Comovirus (1), Cucumovirus (1), Ilarvirus (1), Orthotospovirus (2), Potyvirus (5), Sobemovirus (1), and Tobamovirus (1) [3][4][5][6][7][8][9][10].…”