Characterization of <i>Pseudomonas aeruginosa</i> phage KPP21 belonging to family <i>Podoviridae</i> genus N4‐like viruses isolated in Japan

Shigehisa, Ryu; Uchiyama, Jumpei; Kato, Shin‐ichiro; Takemura‐Uchiyama, Iyo; Yamaguchi, Kotoe; Miyata, Reina; Ujihara, Takako; Sakaguchi, Yoshihiko; Okamoto, Noriaki; Shimakura, Hidekatsu; Daibata, Masanori; Sakaguchi, Masahiro; Matsuzaki, Shigenobu

doi:10.1111/1348-0421.12347

Cited by 12 publications

(10 citation statements)

References 17 publications

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“…To assess the characterization of AM.P2 with respect to previously documented phages, a comparative study of the AM.P2 genome was done. Phylogenetic analysis suggests that the phage is closely related to LUZ7 and KPP21 (19,20), two phages isolated from geographically distant regions (Belgium and Japan, respectively) but that nevertheless share high levels of homology (Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolates

Menon

Babu

et al. 2021

mSphere

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Multidrug-resistant community-acquired infections caused by the opportunistic human pathogen Pseudomonas aeruginosa are increasingly reported in India and other locations globally. Since this organism is ubiquitous in the environment, samples such as sewage and wastewater are rich reservoirs of P. aeruginosa bacteriophages. In this study, we report the isolation and characterization of a novel P. aeruginosa N4-like lytic bacteriophage, vB_Pae_AM.P2 (AM.P2), from wastewater in Kerala, India. AM.P2 is a double-stranded DNA podovirus that efficiently lyses the model strain, PAO1, at a multiplicity of infection as low as 0.1 phage per bacterium and resistance frequency of 6.59 × 10−4. Synergy in bactericidal activity was observed between AM.P2 and subinhibitory concentrations of the antibiotic ciprofloxacin. Genome sequencing of AM.P2 revealed features similar to those of the N4-like P. aeruginosa phages LUZ7 and KPP21. As judged by two independent assay methods, spot tests and growth inhibition, AM.P2 successfully inhibited the growth of almost 30% of strains from a contemporary collection of multidrug-resistant P. aeruginosa clinical isolates from South India. Thus, AM.P2 may represent an intriguing candidate for inclusion in bacteriophage cocktails developed for various applications, including water decontamination and clinical bacteriophage therapy. IMPORTANCE In India, multidrug resistance determinants are much more abundant in community-associated bacterial pathogens due to the improper treatment of domestic and industrial effluents. In particular, a high bacterial load of the opportunistic pathogen P. aeruginosa in sewage and water bodies in India is well documented. The isolation and characterization of bacteriophages that could target emerging P. aeruginosa strains, representing possible epicenters for community-acquired infections, could serve as a useful alternative tool for various applications, such as phage therapy and environmental treatment. Continuing to supplement the repertoire of broad-spectrum bacteriophages is an essential tool in confronting this problem.

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Section: Resultsmentioning

confidence: 99%

A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolates

Menon

Babu

et al. 2021

mSphere

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“…Genome sequencing revealed them to be N4-like, sharing a similar gene order and possessing all 18 core genes that were found among all N4-like members. For phage therapy applications involving the treatment of Pseudomonas aeruginosa murine infections, N4-like phages have been deemed to be safe and effective [ 45 ]. This suggested that the three identified phages could be good candidates for biocontrol applications.…”

Section: Discussionmentioning

confidence: 99%

Novel N4-Like Bacteriophages of Pectobacterium atrosepticum

et al. 2018

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Pectobacterium atrosepticum is an economically important phytopathogen that is responsible for potato blackleg and soft rot, and for which current control strategies are limited. In this study, stem samples of potato crops exhibiting blackleg were taken from three farms in Co. Cork, Ireland, and they were found to be infected with P. atrosepticum. Three closely related bacteriophages (phages) that are specific to this phytopathogen were isolated and characterized, namely vB_PatP_CB1, vB_PatP_CB3, and vB_PatP_CB4 (abbreviated as CB1, CB3, and CB4). Both CB1 and CB3 were determined to infect 12 strains and CB4 10 strains of the 19 strains of P. atrosepticum tested. Morphology, latent periods, burst sizes, and their stability at various temperatures and pHs were also examined. Genome sequencing of the three phages revealed that they shared a minimum nucleotide identity of 93% with each other. Their genomes exhibited an Enquartavirinae genome organization, possessing several conserved proteins that were associated with phages of this group, like the type species Escherichia virus N4. Tandem electrospray ionization-mass spectrometry (ESI-MS/MS) allowed for the identification of ten structural proteins that form the virion of CB1, six that are conserved in phage N4. Biocontrol experiments demonstrated that the phages suppress soft rot formation upon co-inoculation with P. atrosepticum on whole tubers. The results of this study indicate that CB1 related phages could be good candidates for phage-based control.

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“…The G7-like phages The E. coli phage N4, the prototype species of the group of N4-related viruses was a database orphan for many years (Kazmierczak et al 2002). However, over the last decade, numerous N4-like viruses that infect Gram-negative bacteria were isolated and sequenced (Faidiuk et al 2017;Golomidova et al 2007;Cai et al 2015;Patel et al 2015;Li et al 2016;Ma et al 2016;Shigehisa et al 2016;Bhattacharjee et al 2017;Katharios et al 2017;Buttimer et al 2018), and for reviews see (Chan et al 2014;Wittmann et al 2015). The N4-related sequences are also ubiquitous in human fecal metagenomes and viromes (Waller et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Phages associated with horses provide new insights into the dominance of lateral gene transfer in virulent bacteriophages evolution in natural systems

Babenko

Golomidova

Иванов

et al. 2019

Preprint

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Tailed bacteriophages (Caudovirales order) are omnipresent on our planet. Their impressive ecological and evolutionary success largely relies on the bacteriophage potential to adapt to great variety of the environmental conditions found in the Biosphere. It is believed that the adaptation of bacteriophages, including short time scale adaptation, is achieved almost exclusively via the (micro)evolution processes. In order to analyze the major mechanisms driving adaptation of phage genomes in a natural habitat we used comparative genomics of G7C-like coliphage isolates obtained during 7 years period from the feces of the horses belonging to a local population. The data suggest that even at this relatively short time scale the impact of various recombination events overwhelms the impact of the accumulation of point mutations. The access to the large pool of the genes of a complex microbial and viral community of the animal gut had major effect on the evolutionary trajectories of these phages. Thus the "real world" bacteriophage evolution mechanisms may differ significantly from those observed in the simplified laboratory model systems.

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Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4‐like viruses isolated in Japan

Cited by 12 publications

References 17 publications

A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolates

A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolates

Novel N4-Like Bacteriophages of Pectobacterium atrosepticum

Phages associated with horses provide new insights into the dominance of lateral gene transfer in virulent bacteriophages evolution in natural systems

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