Characterization of HTLV-I in a T-Cell Line Established From a Patient With Myelopathy

Infection of human synovial cells by human T cell lymphotropic virus type I. Proliferation and granulocyte/macrophage colony-stimulating factor production by synovial cells.

“…The statistical significance of any difference was calculated using student's t test. 14 despite the careful observation of > 10,000 cells (Fig. 1 B).…”

“…After Cocultivation ofhuman synovial cells and HTL V-I-infected Tcells. MT-2 and HCT-I cells were used as the HTLV-I-infected T cell lines. MT-2 was obtained by coculturing peripheral leukemic cells from ATLL patient with normal umbilical cord leukocytes ( 13), and HCT-l is a T cell line established from cells in the cerebro-spinal fluid of a patient with HAM (14). HCT-I cells proliferate dependently on interleukin 2 and are positive for CD4 antigeps on the cell surface.…”

Infection of human synovial cells by human T cell lymphotropic virus type I. Proliferation and granulocyte/macrophage colony-stimulating factor production by synovial cells.

“…The statistical significance of any difference was calculated using student's t test. 14 despite the careful observation of > 10,000 cells (Fig. 1 B).…”

“…After Cocultivation ofhuman synovial cells and HTL V-I-infected Tcells. MT-2 and HCT-I cells were used as the HTLV-I-infected T cell lines. MT-2 was obtained by coculturing peripheral leukemic cells from ATLL patient with normal umbilical cord leukocytes ( 13), and HCT-l is a T cell line established from cells in the cerebro-spinal fluid of a patient with HAM (14). HCT-I cells proliferate dependently on interleukin 2 and are positive for CD4 antigeps on the cell surface.…”

Antitumor effects of chloroquine/hydroxychloroquine mediated by inhibition of the NF-κB signaling pathway through abrogation of autophagic p47 degradation in adult T-cell leukemia/lymphoma cells

“…Human interleukin-2 (IL-2)-independent ATLL cell lines (Su9T01, S1T, and MT1), HTLV-1-negative human T-cell acute lymphoblastic leukemia (T-ALL) cell lines (MOLT4 and JURKAT), and HTLV-1-infected T cell lines derived from HAM-TSP patients (HCT1, HCT4, and HCT5) [ 21 , 22 ] were cultured in complete RPMI-1640 medium (Nacalai Tesque Inc, Kyoto, Japan) supplemented with 10% FBS and 50 μg/ml penicillin (10 U/ml)-streptomycin (10 μg/ml) (Fisher Scientific). The IL2-dependent ATLL cell lines KK1, KOB, and ST1 were cultured in complete RPMI-1640 medium supplemented with 0.75 μg/ml recombinant human IL-2.…”

Inhibitory effect of HTLV‐1 infection on the production of B‐cell activating factors in established follicular dendritic cell‐like cells

“…We used the cell line HCT‐5, which is a CD4 + T‐cell line from the cerebrospinal fluid of a HAM patient 21 , 22 maintained in RPMI 1640 culture medium containing interleukin (IL)‐2, 20% fetal bovine serum (FBS), and penicillin/streptomycin. The cell line MT‐2, which is derived from a patient with ATL, was maintained in RPMI 1640 culture medium containing 20% FBS.…”

Infection of human synovial cells by human T cell lymphotropic virus type I. Proliferation and granulocyte/macrophage colony-stimulating factor production by synovial cells.

“…The statistical significance of any difference was calculated using student's t test. 14 despite the careful observation of > 10,000 cells (Fig. 1 B).…”

“…After Cocultivation ofhuman synovial cells and HTL V-I-infected Tcells. MT-2 and HCT-I cells were used as the HTLV-I-infected T cell lines. MT-2 was obtained by coculturing peripheral leukemic cells from ATLL patient with normal umbilical cord leukocytes ( 13), and HCT-l is a T cell line established from cells in the cerebro-spinal fluid of a patient with HAM (14). HCT-I cells proliferate dependently on interleukin 2 and are positive for CD4 antigeps on the cell surface.…”

Antitumor effects of chloroquine/hydroxychloroquine mediated by inhibition of the NF-κB signaling pathway through abrogation of autophagic p47 degradation in adult T-cell leukemia/lymphoma cells

“…Human interleukin-2 (IL-2)-independent ATLL cell lines (Su9T01, S1T, and MT1), HTLV-1-negative human T-cell acute lymphoblastic leukemia (T-ALL) cell lines (MOLT4 and JURKAT), and HTLV-1-infected T cell lines derived from HAM-TSP patients (HCT1, HCT4, and HCT5) [ 21 , 22 ] were cultured in complete RPMI-1640 medium (Nacalai Tesque Inc, Kyoto, Japan) supplemented with 10% FBS and 50 μg/ml penicillin (10 U/ml)-streptomycin (10 μg/ml) (Fisher Scientific). The IL2-dependent ATLL cell lines KK1, KOB, and ST1 were cultured in complete RPMI-1640 medium supplemented with 0.75 μg/ml recombinant human IL-2.…”

Inhibitory effect of HTLV‐1 infection on the production of B‐cell activating factors in established follicular dendritic cell‐like cells

“…We used the cell line HCT‐5, which is a CD4 + T‐cell line from the cerebrospinal fluid of a HAM patient 21 , 22 maintained in RPMI 1640 culture medium containing interleukin (IL)‐2, 20% fetal bovine serum (FBS), and penicillin/streptomycin. The cell line MT‐2, which is derived from a patient with ATL, was maintained in RPMI 1640 culture medium containing 20% FBS.…”

Infection of human synovial cells by human T cell lymphotropic virus type I. Proliferation and granulocyte/macrophage colony-stimulating factor production by synovial cells.

“…The statistical significance of any difference was calculated using student's t test. 14 despite the careful observation of > 10,000 cells (Fig. 1 B).…”

“…After Cocultivation ofhuman synovial cells and HTL V-I-infected Tcells. MT-2 and HCT-I cells were used as the HTLV-I-infected T cell lines. MT-2 was obtained by coculturing peripheral leukemic cells from ATLL patient with normal umbilical cord leukocytes ( 13), and HCT-l is a T cell line established from cells in the cerebro-spinal fluid of a patient with HAM (14). HCT-I cells proliferate dependently on interleukin 2 and are positive for CD4 antigeps on the cell surface.…”

Antitumor effects of chloroquine/hydroxychloroquine mediated by inhibition of the NF-κB signaling pathway through abrogation of autophagic p47 degradation in adult T-cell leukemia/lymphoma cells

“…Human interleukin-2 (IL-2)-independent ATLL cell lines (Su9T01, S1T, and MT1), HTLV-1-negative human T-cell acute lymphoblastic leukemia (T-ALL) cell lines (MOLT4 and JURKAT), and HTLV-1-infected T cell lines derived from HAM-TSP patients (HCT1, HCT4, and HCT5) [ 21 , 22 ] were cultured in complete RPMI-1640 medium (Nacalai Tesque Inc, Kyoto, Japan) supplemented with 10% FBS and 50 μg/ml penicillin (10 U/ml)-streptomycin (10 μg/ml) (Fisher Scientific). The IL2-dependent ATLL cell lines KK1, KOB, and ST1 were cultured in complete RPMI-1640 medium supplemented with 0.75 μg/ml recombinant human IL-2.…”

Inhibitory effect of HTLV‐1 infection on the production of B‐cell activating factors in established follicular dendritic cell‐like cells

“…We used the cell line HCT‐5, which is a CD4 + T‐cell line from the cerebrospinal fluid of a HAM patient 21 , 22 maintained in RPMI 1640 culture medium containing interleukin (IL)‐2, 20% fetal bovine serum (FBS), and penicillin/streptomycin. The cell line MT‐2, which is derived from a patient with ATL, was maintained in RPMI 1640 culture medium containing 20% FBS.…”