Characterization of hepatitis A virus isolates from subgenotypes IA and IB in Rio de Janeiro, Brazil*

Paula, Vanessa Salete de; Baptista, Márcia L.; Lampe, Elisabeth; Niel, Christian; Gaspar, Ana

doi:10.1002/jmv.2106

Cited by 66 publications

(66 citation statements)

References 25 publications

(19 reference statements)

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“…In this study, carried out over eight years with patients suspected of having hepatitis, the rate of HAV acute infection positivity (anti-HAV-IgM positive) was 46.4%. This result is similar to those of other studies carried out in Brazil (Cardoso et al 2000, de Paula et al 2002, Villar et al 2002a, even when considering the viral RNA positivity in the analyzed serum samples (51.9%).…”

Section: Discussionsupporting

confidence: 92%

“…This result differs from those of other studies, which show greater molecular marker positivity from anti-HAV-IgM positive samples (Fujiwara et al 1997, Kwon et al 2000, de Paula et al 2002, 2004a, b, Villar et al 2004, Poovorawan et al 2005. This difference could be explained by the fact that the serum samples were stored for a long time and for this reason, the viral RNA could have been degraded.…”

Section: Discussioncontrasting

confidence: 64%

See 1 more Smart Citation

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Fiaccadori¹,

Soares

Borges³

et al. 2006

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 64%

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Fiaccadori¹,

Soares

Borges³

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…Phylogenetic analysis of the nucleotide sequence of the VP1/2A region of the HAV genome classified all isolates as genotype I, confirming former epidemiological data demonstrating that this is the main genotype circulating in Brazil (7,10,22) as well the most widespread genotype in the world (2,23,24). Additionally, we identified the simultaneous presence of sub-genotypes IA and IB in 65 and 35% of cases of HAV infection, respectively.…”

Section: Discussionsupporting

confidence: 86%

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Villar

Morais

Aloise

et al. 2006

Braz J Med Biol Res

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The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis of the VP1/2A sequences showed that 65% belong to sub-genotype IA and 35% to sub-genotype IB. Co-circulation of both sub-genotypes was observed in the two years studied. Distinct clusters of highly related sequences were observed in both sub-genotypes, suggesting endemic circulation of HAV strains in this area. In the 5'NTR isolates, 92.7-99.2% identity was observed and two isolates presented one deletion at position 413. Phylogenetic analysis showed that genotype IA strains cluster in the tree in the same way as genotype IB strains, but one IIIA isolate from Spain clusters with genotype IB strains. These results do not allow us to state that 5'NTR could be used to genotype HAV sequences. This is the first report of co-circulation of sub-genotypes IA and IB in this region, providing additional information about the molecular epidemiology of HAV strains in Brazil.

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“…In an attempt to better characterize the HAF-203 genotype, its nucleotide sequence was compared to 25 complete nucleotide sequences available in databanks. The HAF-203 strain displays a high degree of homology with HM-175, its attenuated derivative, IVA and MBB strains (99.7,99.5,98.8,and 94.8% respectively), confirming previous data (de Paula et al 2002, Lu et al 2004, Stene-Johansen et al 2005 in which HAF-203 was assigned to genotype IB.…”

Section: Genetic Relationship Between Haf-203 and Othersupporting

confidence: 86%

Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Baptista¹,

Silva²,

Lima³

et al. 2006

Mem. Inst. Oswaldo Cruz

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The hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolate in Brazil. The aim of our study was to determine the nucleotide sequence of the HAF-203 strain genome, compare it to other HAV genomes and highlight its genetic variability. The complete nucleotide sequence of the HAF-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other HAV isolates. These analyses revealed 19 HAF-specific nucleotide sequence differences with 10 amino acid substitutions. Most of the non-conservative changes were located at VP1, 2C, and 3D genes, but the 3B region was the most variable. The availability of HAF-203 complementary DNA was useful for the production of the recombinant VP1 protein, which is a major determinant of viral infectivity. This recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests. Key words: hepatitis A virus (HAV) -HAF-203 strain -complete genome (Brazil) -VP1 gene recombinant proteinEscherichia coli expression -HAV diagnosisThe hepatitis A virus (HAV) is the causative agent of an old and common human disease, with high incidence rates, still present in many countries throughout the world. In Brazil, this disease has been responsible for 50% of the acute hepatitis cases referred to the National Reference Center for Viral Hepatitis/Oswaldo Cruz Institute/Ministry of Health. The etiological agent was first identified by Feinstone et al. (1973), and the virus is currently classified as the first and unique member of the genus Hepatovirus, Picornaviridae family (Minor 1991).HAV is a 27-nm non-enveloped icosahedral particle, which comprises a single stranded plus-sense RNA (Coulepis et al. 1981). The viral genome is composed of approximately 7500 nucleotides, edged by a covalently linked VPg protein at the 5' terminal and a polyadenylated 3' end. The viral RNA encodes a single polyprotein of 2224 amino acids, which is later cleaved into 11 viral polypeptides (Cohen et al. 19847a).The Brazilian HAF-203 strain investigated here was obtained from a faecal specimen of an acute hepatitis A patient. The viral isolation and serial passage history was previous reported by Gaspar et al. (1992). The HAF-203 HAV strain was inoculated into fetal Rhesus kidney cells (Baptista ML et al. unpublished). In the present study, we have sequenced the complete genome of the HAF-203 HAV strain and compared it with other HAV isolates. Several groups have adopted recombinant viral proteins for diagnostic purposes (Ostermayer et al. 1987, Johnston et al. 1988, LaBrecque et al. 1998, Ternovai et al. 2001, Di Napoli et al. 2004. A fragment of the HAF-203 strain encoding the complete VP1 gene was cloned and expressed in E...

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Characterization of hepatitis A virus isolates from subgenotypes IA and IB in Rio de Janeiro, Brazil*

Cited by 66 publications

References 25 publications

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Prevalence of hepatitis A virus infection in Goiânia, Goiás, Brazil, by molecular and serological procedures, 1995-2002

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

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