Characterization of flupyradifurone resistance in the whitefly <scp><i>Bemisia tabaci</i></scp> Mediterranean (Q biotype)

Wang, Ran; Wang, Jinda; Zhang, Jiasong; Che, Wunan; Feng, Honglin; Luo, Chen

doi:10.1002/ps.5995

Cited by 37 publications

(28 citation statements)

References 45 publications

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“…This study concerns the natural occurrence of whitefly secondary symbionts and the fitness costs/benefits associated with their infections in the B. tabaci whitefly, focusing on the invasive MED species, which is one group of notorious agricultural pests worldwide having developed high resistance to insecticide ( Wang et al, 2020a , b ). The MED whiteflies includes four phylogenetic clades Q1, Q2, Q3, and ASL, and Q1 is the most widely distributed genotype in most regions, especially in China ( Chu et al, 2008 ; Gueguen et al, 2010 ; Gauthier et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field

Shan

Liu

2021

Front. Microbiol.

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Insects commonly harbor maternally inherited intracellular symbionts in nature, and the microbial partners often exert influence on host reproduction and fitness to promote their prevalence. Here, we investigated composition of symbionts and their biological effects in the invasive Bemisia tabaci MED species of a whitefly complex. Our field surveys revealed that populations of the MED whitefly, in addition to the primary symbiont Portiera, mainly contain two secondary symbionts Hamiltonella, which is nearly fixed in the host populations, and Cardinium with infection frequencies ranging from 0 to 86%. We isolated and established Cardinium-positive and Cardinium-free whitefly lines with a similar nuclear genetic background from a field population, and compared performance of the two whitefly lines. The infection of Cardinium incurred significant fitness costs on the MED whitefly, including reduction of fecundity and egg viability as well as delay in development. We then selectively removed Hamiltonella from the Cardinium-free whitefly line and compared performance of two whitefly lines, one harboring both Portiera and Hamiltonella and the other harboring only Portiera. While depletion of Hamiltonella had little or only marginal effects on the fecundity, developmental rate, and offspring survival, the Hamiltonella-free whitefly line produced very few female offspring, often reducing the progeny female ratio from about 50% to less than 1%. Our findings indicate that the varying costs and benefits of the association between these two symbionts and the MED whitefly may play an important role in shaping their differential prevalence in the field.

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Section: Introductionmentioning

confidence: 99%

The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field

Shan

Liu

2021

Front. Microbiol.

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“…The resistance levels were low in a heterozygous Drosophila strain (RFs of 1.9, 1.3 and 4.3, respectively). Finally, metabolic resistance to flupyradifurone, mediated by glutathione‐S‐transferase and P450 monooxygenase, recently was suggested for a Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) resistant strain from China 26 …”

Section: Introductionmentioning

confidence: 99%

Flupyradifurone resistance in Myzus persicae populations from peach and tobacco in Greece

Papadimitriou

Folia²,

Ilias

et al. 2021

Pest Management Science

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BACKGROUND Myzus persicae has evolved resistance to various insecticides in Greece. Here we examine the effectiveness of the insecticide flupyradifurone against aphid clones collected from tobacco and peach in Greece during 2017–2020. Furthermore, we monitored the frequency of the neonicotinoid resistance mutation R81T in the sampled clones, and the association between the responses to flupyradifurone and acetamiprid. RESULTS Of 43 clones tested with flupyradifurone, 6.977%, 60.465% and 32.558% showed low (10–14), moderate (19–89) and high (104–1914) resistance factor (RF) values, respectively. Resistance was higher in clones from peach than from tobacco with 42.308% and 17.647% of clones (respectively) failing into the high RF category (median RF values 67.5 and 36.4 for clones from peach and tobacco, respectively). Acetamiprid resistance was detected in clones collected in 2019–2020, in line with our previous study in Greece. The analysis of the whole dataset (54 clones collected during 2017–2020) revealed that all tobacco clones had RF < 7.5, whereas 55.263%, 18.421% and 26.316% of the peach clones exhibited low (<12), moderate (20–48) and high (100–145) RF values, respectively. A significant but moderate association between flupyradifurone and acetamiprid responses was detected (r = 0.513, P < 0.001). The R81T mutation was detected in aphids from peach (5.6% and 32.6% as homozygotes and heterozygotes, respectively) and in one aphid specimen (heterozygote) from tobacco. R81T was partially associated with the resistance to both insecticides, but many highly resistant clones did not possess the mutation, indicating the possible operation of one or more alternative underlying resistance mechanisms. CONCLUSIONS The use of flupyradifurone and acetamiprid in IPM/IRM should be based on further ongoing susceptibility monitoring. © 2021 Society of Chemical Industry.

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“…In addition, RNA interference (RNAi)-based studies have identified several cases of insecticide resistance involving P450s. For example, CYP6CX4 is connected with the resistance to flupyrrolidone in Bemisia tabaci [25]; CYP6CY14, CYP6DC1, and CYP6CZ1 are associated with Aphis gossypii resistance to acetamiprid [26]; CYP321A8, CYP321A9, and CYP321B1 are responsible for Spodoptera frugiperda resistance to chlorantraniliprole [27]. In Drosophila melanogaster, Cyp4g1, Cyp6g1, and Cyp12d1 play major roles in reducing DDT sensitivity [28].…”

Section: P450-mediated Insecticide Resistancementioning

confidence: 99%

The Role of Insect Cytochrome P450s in Mediating Insecticide Resistance

Nayak

Xiong

et al. 2022

Agriculture

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In many organisms, cytochrome P450 enzymes are the primary detoxifying enzymes. Enhanced P450 activity can be mediated by the emergence of new genes, increased transcription due to mutations in the promoter regions, changes in enzyme structures and functions due to mutations in protein-coding regions, or changes in post-translational modifications; all of these changes are subject to insecticide selection pressure. Multiple signalling pathways and key effector molecules are involved in the regulation of insect P450s. Increased P450 activity is a key mechanism inducing insect resistance. Hence, downregulation of selected P450s is a promising strategy to overcome this resistance. Insect P450 inhibitors that act as insecticide synergists, RNA interference to induce P450 gene silencing, and the use of transgenic insects and crops are examples of strategies utilized to overcome resistance. This article reviews the latest advances in studies related to insect P450s-mediated agrochemical resistance, with focuses on the regulatory mechanisms and associated pest management strategies. Future investigations on the comprehensive regulatory pathways of P450-mediated detoxification, identification of key effectors, and downregulation strategies for P450s will ecologically, economically, and practically improve pest management.

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Characterization of flupyradifurone resistance in the whitefly Bemisia tabaci Mediterranean (Q biotype)

Cited by 37 publications

References 45 publications

The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field

The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field

Flupyradifurone resistance in Myzus persicae populations from peach and tobacco in Greece

The Role of Insect Cytochrome P450s in Mediating Insecticide Resistance

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